Colostrum Management for Dairy Calves.

Colostrum management is the single most important management factor in determining calf health and survival. Additional benefits of good colostrum management include improved rate of gain and future productivity. Successful colostrum management requires producers to provide calves with a sufficient volume of clean, high-quality colostrum within the first few hours of life. This article reviews the process of colostrogenesis and colostrum composition, and discusses key components in developing a successful colostrum management program. In addition, the article discusses approaches for monitoring and proposes new goals for passive immunity in dairy herds.

Evaluation of the risk of paratuberculosis in adult cows fed Mycobacterium avium subsp paratuberculosis DNA-positive or -negative colostrum as calves.

OBJECTIVE: To estimate the risk of subclinical Mycobacterium avium subsp paratuberculosis (MAP) infection in cows that ingested MAP DNA-positive raw colostrum as calves, compared with risk in cows that ingested MAP DNA-negative raw colostrum as calves. ANIMALS: 205 calves born in 12 commercial dairy herds. PROCEDURES: Each calf was separated from its dam within 30 to 60 minutes after birth and fed raw colostrum. For each calf, samples of the colostrum fed were collected and tested for the presence of MAP DNA by use of a nested PCR assay for the target gene ISMAP02. Calves fed colostrum positive or negative for MAP DNA were classified into exposed (n = 69) and unexposed (136) groups, respectively. Each calf was tested for MAP infection at 30, 42, and 54 months of age by use of a serum ELISA and bacterial culture of feces. Weibull hazard regression models were used to evaluate the association between exposure to MAP DNA-positive colostrum and time to testing positive for MAP infection. RESULTS: Hazard of MAP infection was not different between groups (exposed vs unexposed) when serum ELISA, bacterial culture of feces, or both diagnostic tests (parallel interpretation) were positive. CONCLUSIONS AND CLINICAL RELEVANCE: Heifer calves fed MAP DNA-positive colostrum were at no greater risk of MAP infection, compared with heifer calves fed MAP DNA-negative colostrum. This result contradicts findings from other studies and should be interpreted with caution.

Evaluation of the association between fecal excretion of Mycobacterium avium subsp paratuberculosis and detection in colostrum and on teat skin surfaces of dairy cows.

OBJECTIVE: To evaluate the association between fecal excretion of Mycobacterium avium subsp paratuberculosis (MAP) by dairy cows in the periparturient period and detection of MAP DNA in colostrum specimens and on teat skin surfaces. DESIGN: Cross-sectional study. ANIMALS: 112 Holstein cows. PROCEDURES: Fecal specimens were collected within 48 to 72 hours prior to parturition, and colostrum and teat swab specimens were collected immediately after parturition. Detection of MAP in fecal specimens was performed via microbial culture, and detection of MAP DNA in colostrum and teat swab specimens was achieved via a PCR assay targeting the genetic element ISMAP02. Logistic regression was used to model the relationship between MAP fecal shedding status and detection of MAP DNA in colostrum or teat swab specimens. Population attributable fractions for the proportion of colostrum and teat swab specimens containing MAP DNA were also calculated. RESULTS: The odds of detecting MAP DNA in colostrum or teat swab specimens in cows with MAP-positive (vs negative) fecal specimens were 2.02 and 1.87 respectively. Population attributable fractions estimates suggested that withholding colostrum from MAP-positive cows could reduce the odds of exposing calves to MAP in colostrum by 18.2%. Not permitting natural suckling by calves could reduce the odds of exposing calves to MAP on the teat surfaces of MAP-positive cows by 19.5%. CONCLUSIONS AND CLINICAL RELEVANCE: Results underscored the need for strict adherence to practices that limit contact of calves with adult cows from the time of birth and promote hygienic colostrum handling to avoid possible contamination with MAP during colostrum harvest, storage, or feeding.

Effect of a plasma-derived colostrum replacement feeding program on adult performance and longevity in Holstein cows.

OBJECTIVE: To evaluate longevity, milk production, and breeding performance in adult Holstein cows fed either a plasma-derived commercial colostrum replacer (CR) or raw bovine maternal colostrum (MC) at birth. DESIGN: Randomized controlled clinical trial. ANIMALS: 497 heifer calves born in 12 commercial dairies located in Minnesota and Wisconsin. PROCEDURES: All calves were separated from their dams within 30 to 60 minutes after birth and systematically assigned to be fed either MC (control group [n = 261 calves]) or CR (treatment group [236]). Calves were observed from birth up to adulthood (approx 54 months old), during which time death and culling events plus milk yield and breeding performance data were collected. Time to death, time to culling, time to death or culling combined, time to first calving, and time to conception intervals were evaluated by use of proportional hazards survival analysis models. Number of times inseminated per conception and lifetime milk yield (up to 54 months old) were evaluated by use of general linear models. RESULTS: Cows fed CR as calves at the time of birth were no different than cows fed MC as calves with respect to overall risk of death, culling, or death or culling combined (from birth to 54 months of follow-up and from first calving to 54 months old); lifetime milk yield; and breeding performance. CONCLUSIONS AND CLINICAL RELEVANCE: No difference was detected in overall risk of death or culling, milk production, or reproductive performance between cows fed CR and those fed MC as calves at birth.

Experimental validation of a nested polymerase chain reaction targeting the genetic element ISMAP02 for detection of Mycobacterium avium subspecies paratuberculosis in bovine colostrum.

Colostrum samples experimentally inoculated with Mycobacterium avium subsp. paratuberculosis (MAP; strain K-10) at increasing concentrations between 1 x 10(1) and 1 x 10(9) cells/ml were tested for recovery of MAP DNA using a nested ISMAP02 target polymerase chain reaction initially developed for detecting MAP DNA in fecal samples. The following detection rates were achieved for sample replicates inoculated with unsonicated MAP pure stock: 100% between 1 x 10(7) and 1 x 10(9) cells/ml, 75% between 1 x 10(3) and 1 x 10(6) cells/ml, and 50% between 1 x 10(1)and 1 x 10(2) cells/ml replicates. Detection rates achieved for the colostrum sample replicates inoculated with sonicated MAP cell suspension were 75% for 1 x 10(9) cells/ml, 100% between 1 x 10(7) and 1 x 10(8) cells/ml, 75% for 1 x 10(6) cells/ml, 0 for 1 x 10(4) cells/ml, and 25% between 1 x 10(1) and 1 x 10(3) cells/ml. When negative control colostrum samples were tested, 16 of 18 (89%) samples were correctly detected as negative for MAP DNA using the current assay. In conclusion, the MAP DNA detection rates of the present assay improved with increasing concentrations of MAP in the colostrum sample replicates, although MAP DNA was also detected in 2 of 18 (11%) negative control samples, suggesting an undefined technical problem with the assay or, perhaps, sample contamination during preparation. Overall, the present findings suggest a potential role of the proposed polymerase chain reaction assay to detect MAP in colostrum. However, adoption of this test for use in routine screening of field colostrum for MAP awaits findings from an ongoing field validation study.

Efficacy of feeding plasma-derived commercial colostrum replacer for the prevention of transmission of Mycobacterium avium subsp paratuberculosis in Holstein calves.

OBJECTIVE: To estimate the relative risk of paratuberculosis (Johne's disease [JD]) in calves fed a plasma-derived colostrum-replacement (CR) product versus raw bovine maternal colostrum (MC). STUDY DESIGN: Randomized controlled clinical trial. ANIMALS: 497 heifer calves born in 12 JD-endemic commercial Holstein dairy farms located in Minnesota and Wisconsin. PROCEDURES: Every calf was separated from its dam within 30 to 60 minutes after birth and systematically assigned to be fed raw bovine MC (control group, n = 261 calves) or CR (treatment group, 236 calves). The calves were monitored to adulthood and tested for Mycobacterium avium subsp paratuberculosis (MAP) infection by use of an ELISA to detect serum antibodies against MAP and bacterial culture for MAP in feces at approximately 30, 42, and 54 months of age. Weibull regression models were used to evaluate the effect of feeding CR (vs raw bovine MC) on the risk of developing JD infection. RESULTS: Calves fed CR at birth were less likely (hazard ratio = 0.559) to become infected with MAP (as determined by use of an ELISA, bacterial culture, or both diagnostic tests), compared with the likelihood for calves fed MC at birth. CONCLUSIONS AND CLINICAL RELEVANCE: This study revealed that feeding CR reduced the risk of developing MAP infection in Holstein calves born in JD-endemic herds, which implied that feeding raw bovine MC may be a source of MAP for calves. Plasma colostrum-replacement products may be an effective management tool for use in dairy herds attempting to reduce the prevalence of JD.