Enhanced Bio-P removal: Past, present, and future - A comprehensive review.

Excess amounts of phosphorus (P) and nitrogen (N) from anthropogenic activities such as population growth, municipal and industrial wastewater discharges, agriculture fertilization and storm water runoffs, have affected surface water chemistry, resulting in episodes of eutrophication. Enhanced biological phosphorus removal (EBPR) based treatment processes are an economical and environmentally friendly solution to address the present environmental impacts caused by excess P present in municipal discharges. EBPR practices have been researched and operated for more than five decades worldwide, with promising results in decreasing orthophosphate to acceptable levels. The advent of molecular tools targeting bacterial genomic deoxyribonucleic acid (DNA) has also helped us reveal the identity of potential polyphosphate-accumulating organisms (PAO) and denitrifying PAO (DPAO) responsible for the success of EBPR. Integration of process engineering and environmental microbiology has provided much-needed confidence to the wastewater community for the successful implementation of EBPR practices around the globe. Despite these successes, the process of EBPR continues to evolve in terms of its microbiology and application in light of other biological processes such as anaerobic ammonia oxidation and on-site carbon capture. This review provides an overview of the history of EBPR, discusses different operational parameters critical for the successful operation of EBPR systems, reviews current knowledge of EBPR microbiology, the influence of PAO/DPAO on the disintegration of microbial communities, stoichiometry, EBPR clades, current practices, and upcoming potential innovations.

Per and poly-fluoroalkyl substances (PFAS) as a contaminant of emerging concern in surface water: A transboundary review of their occurrences and toxicity effects.

Per and poly-fluoroalkyl substances (PFAS) have been recognized as contaminants of emerging concerns by the United States Environmental Protection Agency (US EPA) due to their environmental impact. Several advisory guidelines were proposed worldwide aimed at limiting their occurrences in the aquatic environments, especially for perfluorooctane sulfonic acid (PFOS) and perfluorooctanoic acid (PFOA). This review paper aims to provide a holistic review in the emerging area of PFAS research by summarizing the spatiotemporal variations in PFAS concentrations in surface water systems globally, highlighting the possible trends of occurrences of PFAS, and presenting potential human health impacts as a result of PFAS exposure through surface water matrices. From the data analysis in this study, occurrences of PFOA and PFOS in many surface water matrices were observed to be several folds higher than the US EPA health advisory level of 70 ng/L for lifetime exposure from drinking water. Direct discharge and atmospheric deposition were identified as primary sources of PFAS in surface water and cryosphere, respectively. While global efforts focused on limiting usages of long-chain PFAS such as PFOS and PFOA, the practices of using short-chain PFAS such as perfluorobutanoic acid (PFBA) and perfluorobutane sulfonic acid (PFBS) and PFAS alternatives increased substantially. These compounds are also potentially associated with adverse impacts on human health, animals and biota.

Fate of 17ß-Estradiol as a model estrogen in source separated urine during integrated chemical P recovery and treatment using partial nitritation-anammox process.

Recently, research on source separation followed by the treatment of urine and/or resource recovery from human urine has shown promise as an emerging management strategy. Despite contributing only 1% of the total volume of wastewater, human urine contributes about 80% of the nitrogen, 70% of the potassium, and up to 50% of the total phosphorus in wastewater. It is also a known fact that many of the micropollutants, especially selected estrogens, get into municipal wastewater through urine excretion. In this research, we investigated the fate of 17ß-estradiol (E2) as a model estrogen during struvite precipitation from synthetic urine followed by the treatment of urine using a partial nitritation-anammox (PN/A) system. Single-stage and two-stage suspended growth PN/A configurations were used to remove the nitrogen in urine after struvite precipitation. The results showed an almost 95% phosphorous and 5% nitrogen recovery/removal from the synthetic urine due to struvite precipitation. The single and two stage PN/A processes were able to remove around 50% and 75% of ammonia and nitrogen present in the post struvite urine solution, respectively. After struvite precipitation, more than 95% of the E2 remained in solution and the transformation of E2 to E1 happened during urine storage. Most of the E2 removal that occurred during the PN/A process was due to sorption on the biomass and biodegradation (transformation of E2 to E1, and slow degradation of E1 to other metabolites). These results demonstrate that a combination of chemical and biological unit processes will be needed to recover and manage nutrients in source separated urine.

Response of a sludge-minimizing lab-scale BNR reactor when the operation is changed to real primary effluent from synthetic wastewater.

The activated sludge process is the most widely used treatment method for municipal wastewater. However, the excessive amount of biomass generated during the process is a major drawback. Earlier studies using the activated sludge process running in a biomass fasting and feasting mode demonstrated both nutrient removal and a minimization of biomass production. However, these studies were conducted using synthetic wastewater. In this study, we report findings from a lab-scale sludge-minimizing biological nutrient removing (BNR) reactor when its operation was changed from synthetic to real wastewater (primary effluent). Two lab-scale sequencing batch reactors, one in sludge minimization mode (hereafter called modified-SBR), and the other in conventional activated sludge mode (referred as control-SBR), were operated for more than 300 days. Both reactors were started and operated with synthetic feed. Gradually the feed to both reactors was changed to 100% primary effluent collected from a local full-scale wastewater treatment plant. Irrespective of the feed composition, more than 98% NH3-N removal was recorded in both SBRs. However, while 89% of the total dissolved phosphorus was removed from the 100% synthetic feed, only 80% of the total dissolved phosphorus was removed from the 100% primary effluent in both SBRs. The overall observed sludge reduction in the modified-SBR as compared to the control-SBR also decreased from 65% to 39% when the feed was changed from 100% synthetic to 100% primary effluent. The specific oxygen uptake rate for the modified-SBR was 80% higher than that for the control-SBR when the SBRs were fed with primary effluent wastewater. The modified-SBR showed a greater diversity of ammonia-oxidizing bacteria (AOBs) with synthetic wastewater as well as during the transition period than the control-SBR. Yet when the reactors were running on 100% real wastewater, only Nitrosomonas europaea/eutropha were identified in both SBRs. The nitrite-oxidizing bacterial community and the polyphosphate accumulating organisms (PAOs) responded in a similar way in both SBRs.

Microbiological study of bacteriophage induction in the presence of chemical stress factors in enhanced biological phosphorus removal (EBPR).

Polyphosphate accumulating organisms (PAOs) are responsible for carrying the enhanced biological phosphorus removal (EBPR). Although the EBPR process is well studied, the failure of EBPR performance at both laboratory and full-scale plants has revealed a lack of knowledge about the ecological and microbiological aspects of EBPR processes. Bacteriophages are viruses that infect bacteria as their sole host. Bacteriophage infection of polyphosphate accumulating organisms (PAOs) has not been considered as a main contributor to biological phosphorus removal upsets. This study examined the effects of different stress factors on the dynamics of bacteriophages and the corresponding effects on the phosphorus removal performance in a lab-scale EBPR system. The results showed that copper (heavy metal), cyanide (toxic chemical), and ciprofloxacin (antibiotic), as three different anthropogenic stress factors, can induce phages integrated onto bacterial genomes (i.e. prophages) in an enriched EBPR sequencing batch reactor, resulting in a decrease in the polyphosphate kinase gene ppk1 clades copy number, phosphorus accumulation capacity, and phosphorus removal performance. This study opens opportunities for further research on the effects of bacteriophages in nutrient cycles both in controlled systems such as wastewater treatment plants and natural ecosystems.

Evaluation of simultaneous nutrient removal and sludge reduction using laboratory scale sequencing batch reactors.

The treatment and disposal of excess sludge has been a rising challenge for wastewater treatment plants worldwide. In this study, simultaneous sludge reduction and nutrient removal was evaluated in laboratory scale sequencing batch reactors (SBRs). Two SBRs were operated alongside for a duration of 370d. One SBR was operated to achieve nutrient removal (control-SBR) at 10d solids retention time (SRT), while the other (modified-SBR) was operated to achieve nutrient removal along with sludge reduction. Sludge reduction in the modified-SBR was accomplished by subjecting the recycled biomass to feasting and fasting at sufficiently high SRT close to infinity (phase I and II) and finite SRT (phase III). The observed biomass yield in the modified-SBR was estimated to be 0.17mg TSSmg(-1) COD, representing 63% sludge reduction compared to the control-SBR. The NH(3) levels in the effluents from both SBRs always remained below detection limit. The average dissolved phosphorus removal efficiencies in the control-SBR and the modified-SBR were 87% and 84%, respectively, during phase II. However, the biomass of the modified-SBR increased during phase II. To control this, biomass wastage was initiated directly from the modified-SBR during phase III at a rate equivalent to the observed biomass accumulation rate in the system in phase II. This resulted in an overall 100d SRT for the modified-SBR system. Following this change, biomass accumulation in the modified-SBR was controlled, and a net 63% sludge reduction could be sustained along with 90% phosphorus and 100% NH3 removal. Consistent denitrification activities were also noticed in both SBRs despite the absence of any carbon source during the anoxic phase of every cycle.