Enhancing salinity tolerance in cucumber through Selenium biofortification and grafting.

BACKGROUND: Salinity stress is a major limiting factor for plant growth, particularly in arid and semi-arid environments. To mitigate the detrimental effects of salinity stress on vegetable production, selenium (Se) biofortification and grafting onto tolerant rootstocks have emerged as effective and sustainable cultivation practices. This study aimed to investigate the combined effects of Se biofortification and grafting onto tolerant rootstock on the yield of cucumber grown under salinity stress greenhouse conditions. The experiment followed a completely randomized factorial design with three factors: salinity level (0, 50, and 100 mM of NaCl), foliar Se application (0, 5, and 10 mg L-1 of sodium selenate) and grafting (grafted and non-grafted plants) using pumpkin (Cucurbita maxima) as the rootstock. Each treatment was triplicated. RESULTS: The results of this study showed that Se biofortification and grafting significantly enhanced salinity tolerance in grafted cucumbers, leading to increased yield and growth. Moreover, under salinity stress conditions, Se-Biofortified plants exhibited increased leaf relative water content (RWC), proline, total soluble sugars, protein, phenol, flavonoids, and antioxidant enzymes. These findings indicate that Se contributes to the stabilization of cucumber cell membrane and the reduction of ion leakage by promoting the synthesis of protective compounds and enhancing antioxidant enzyme activity. Moreover, grafting onto pumpkin resulted in increased salinity tolerance of cucumber through reduced Na uptake and translocation to the scion. CONCLUSION: In conclusion, the results highlight the effectiveness of Se biofortification and grafting onto pumpkin in improving cucumber salinity tolerance. A sodium selenate concentration of 10 mg L-1 is suggested to enhance the salinity tolerance of grafted cucumbers. These findings provide valuable insights for the development of sustainable cultivation practices to mitigate the adverse impact of salinity stress on cucumber production in challenging environments.

Seedling nanopriming with selenium-chitosan nanoparticles mitigates the adverse effects of salt stress by inducing multiple defence pathways in bitter melon plants.

Advances in the nanotechnology fields provided crucial applications in plant sciences, contributing to the plant performance and health under stress and stress-free conditions. Amid the applications, selenium (Se), chitosan and their conjugated forms as nanoparticles (Se-CS NPs) have been revealed to have potential of alleviating the harmful effects of the stress on several crops and subsequently enhancing the growth and productivity. The present study was addressed to assay the potential effects of Se-CS NPs in reversing or buffering the harmful effects of salt stress on growth, photosynthesis, nutrient concentration, antioxidant system and defence transcript levels in bitter melon )Momordica charantia(. In addition, some secondary metabolite-related genes were explicitly examined. In this regard, the transcriptional levels of WRKY1, SOS1, PM H+-ATPase, SKOR, Mc5PTase7, SOAR1, MAP30, α-MMC, polypeptide-P and PAL were quantified. Our results demonstrated that Se-CS NPs increased growth parameters, photosynthesis parameters (SPAD, Fv/Fm, Y(II)), antioxidant enzymatic activity (POD, SOD, CAT) and nutrient homeostasis (Na+/K+, Ca2+, and Cl-) and induced the expression of genes in bitter melon plants under salt stress (p ≤ 0.05). Therefore, applying Se-CS NPs might be a simple and effective way of improving crop plants' overall health and yield under salt stress conditions.

Biological activity and development of functional foods fortified with okra (Abelmoschus esculentus).

The Abelmoschus esculentus plant, better known as okra, is an interesting crop from a nutritional standpoint. The okra plant is native to the African region but can now be found throughout tropical and subtropical areas of the world. This plant, known for its healing abilities, has been used as a traditional medicine to treat several diseases and external ailments, such as wounds or boils. This article reviews the potential health benefits from okra consumption, as well as the bioactive compounds that are suggested to be responsible. Furthermore, the okra plant and its derivatives have been evaluated in the formulation and manufacture of new functional food products. The latest advances in this direction, which includes characterizing the technical properties of functional foods fortified with okra are also presented in this review. A series of bioactive compounds such as flavonoids and catechins have been found in the okra plant, which were associated with numerous biological properties observed in research studies that reported potential anti-diabetic, anti-cancer, anti-hypertensive, and antimicrobial effects, among others, as a result of their consumption. These potential health benefits contribute to the development of new and useful functional foods, with okra (or its derivatives) being used as the highlighted ingredient.

Postharvest chitosan-arginine nanoparticles application ameliorates chilling injury in plum fruit during cold storage by enhancing ROS scavenging system activity.

BACKGROUND: Plum (Prunus domestica L.) has a short shelf-life period due to its high respiration rate and is sensitive to low storage temperatures, which can lead to the appearance of chilling injury symptoms. In this investigation, we applied new coating treatments based on chitosan (CTS) and arginine (Arg) to plum fruit (cv. 'Stanley'). RESULTS: Fruit were treated with distilled water (control), Arg at 0.25 and 0.5 mM, CTS at 1% (w/v) or Arg-coated CTS nanoparticles (CTS-Arg NPs) at 0.5 and 1% (w/v), and then stored at 1 °C for days. The application of CTS-Arg NPs at 0.5% attenuated chilling injury, which was accompanied by accumulation of proline, reduced levels of electrolyte leakage and malondialdehyde, as well as suppressed the activity of polyphenol oxidase. Plums coated with CTS-Arg NPs (0.5%) showed higher accumulation of phenols, flavonoids and anthocyanins, due to the higher activity of phenylalanine ammonia-lyase, which in turn resulted in higher DPPH scavenging capacity. In addition, CTS-Arg NPs (0.5%) treatment delayed plum weight loss and retained fruit firmness and ascorbic acid content in comparison to control fruit. Furthermore, plums treated with CTS-Arg NPs exhibited lower H2O2 accumulation than control fruit due to higher activity of antioxidant enzymes, including CAT, POD, APX and SOD. CONCLUSIONS: The present findings show that CTS-Arg NPs (0.5%) were the most effective treatment in delaying chilling injury and prolonging the shelf life of plum fruit.

Application of Glycine betaine coated chitosan nanoparticles alleviate chilling injury and maintain quality of plum (Prunus domestica L.) fruit.

The use of edible coatings can lead to significant extension of the postharvest life of fresh horticultural products through the regulation of water and gaseous exchange during storage. In this regard, nano-engineered materials are of great interest to design novel and multifunctional edible coatings and are increasingly employed. Chitosan and glycine betaine have been reported to enhance fruit tolerance to chilling stress during cold storage. The current study applied new coating treatments to plum (Prunus domestica L. cv. 'Stanley') fruit at maturity stage in a completely randomized factorial design with three replicates. Plums were treated with distilled water (control), glycine betaine (GB) at 2.5 and 5 mM, chitosan (CTS) at 1% (w/v) or glycine betaine-coated chitosan nanoparticles (CTS-GB NPs) at 0.5 and 1% (w/v) and stored at 1 °C for up to 40 days. The application of CTS-GB NPs (0.5% w/v) was the most effective treatment and induced lower electrolyte leakage, MDA and H2O2 content, and significantly alleviated chilling injury. Furthermore, this treatment remarkably increased the activity of PAL enzyme, resulting in higher levels of phenolics, flavonoids and anthocyanins content, and enhanced DPPH scavenging capacity. In addition, CTS-GB NPs treatment increased endogenous GB (9.25 mg g-1 DW) and proline (1929.29 µg g-1 FW) accumulation leading to higher activity of CAT, POD, SOD and APX enzymes. Based on the obtained results, the commercial application of CTS-GB NPs could effectively reduce chilling injury, preserve nutritional quality, and prolong the storage potential and shelf life of plum fruit.

Chitosan-selenium nanoparticles (Cs-Se NPs) modulate the photosynthesis parameters, antioxidant enzymes activities and essential oils in Dracocephalum moldavica L. under cadmium toxicity stress.

In view of damaging impacts of cadmium (Cd) toxicity on various vital processes of plants and strategies for alleviating these effects, selenium (Se) application has been recently achieved great attention. In addition, chitosan (CS) and its nano-form, besides many positive effects on plants, could be considered as an excellent adsorption matrix and a carrier for a wide range of materials like Se with various applications in agricultural sector. For that point, the combination nano-form of Se and CS (CS-Se NPs), using CS as a carrier and control releaser for Se, could enhance Se efficiency particularly at lower doses under stress conditions. Therefore, Se (10 mg L-1), CS (0.1%) and CS-Se NPs (in two concentrations; 5 and 10 mg L-1) were applied on Moldavian balm plant under 0, 2.5 and 5 mg kg-1 Cd-stress conditions. The results demonstrated that mostly Se and CS-Se NPs treatments could lessen negative effects of Cd-stress conditions through enhancing agronomic traits, photosynthetic pigments, chlorophyll fluorescence parameters and SPAD, proline, phenols, antioxidant enzymes activities and some dominant constituents of essential oils and decreasing MDA and H2O2. These encouraging impacts were more significant at lower dose of CS-Se NPs (5 mg L-1) introducing it as the best treatment to ameliorate Moldavian balm performance under Cd-stress conditions. In conclusion, CS-Se NPs could be considered as a supportive approach in plant production mainly under different heavy metal stressful conditions and probably a potential plant growth promoting and stress protecting agent with new outlooks for applying in agricultural sector.

Salt Stress Mitigation via the Foliar Application of Chitosan-Functionalized Selenium and Anatase Titanium Dioxide Nanoparticles in Stevia (Stevia rebaudiana Bertoni).

High salt levels are one of the significant and major limiting factors on crop yield and productivity. Out of the available attempts made against high salt levels, engineered nanoparticles (NPs) have been widely employed and considered as effective strategies in this regard. Of these NPs, titanium dioxide nanoparticles (TiO2 NPs) and selenium functionalized using chitosan nanoparticles (Cs-Se NPs) were applied for a quite number of plants, but their potential roles for alleviating the adverse effects of salinity on stevia remains unclear. Stevia (Stevia rebaudiana Bertoni) is one of the reputed medicinal plants due to their diterpenoid steviol glycosides (stevioside and rebaudioside A). For this reason, the current study was designed to investigate the potential of TiO2 NPs (0, 100 and 200 mg L-1) and Cs-Se NPs (0, 10 and 20 mg L-1) to alleviate salt stress (0, 50 and 100 mM NaCl) in stevia. The findings of the study revealed that salinity decreased the growth and photosynthetic traits but resulted in substantial cell damage through increasing H2O2 and MDA content, as well as electrolyte leakage (EL). However, the application of TiO2 NPs (100 mg L-1) and Cs-Se NPs (20 mg L-1) increased the growth, photosynthetic performance and activity of antioxidant enzymes, and decreased the contents of H2O2, MDA and EL under the saline conditions. In addition to the enhanced growth and physiological performance of the plant, the essential oil content was also increased with the treatments of TiO2 (100 mg L-1) and Cs-Se NPs (20 mg L-1). In addition, the tested NPs treatments increased the concentration of stevioside (in the non-saline condition and under salinity stress) and rebaudioside A (under the salinity conditions) in stevia plants. Overall, the current findings suggest that especially 100 mg L-1 TiO2 NPs and 20 mg L-1 Cs-Se could be considered as promising agents in combating high levels of salinity in the case of stevia.

Modified multiwall carbon nanotubes display either phytotoxic or growth promoting and stress protecting activity in Ocimum basilicum L. in a concentration-dependent manner.

Carbon-based materials including multiwall carbon nanotubes (MWCNTs) have been recently implicated in a number of reports dealing with their potential use in agriculture, leading to contradictory findings. In this study, MWCNTs were successfully functionalized with carboxylic acid groups (MWCNTs-COOH) in order to increase water dispersion. Hydroponically cultured sweet basil (Ocimum basilicum L.) seedlings were subjected to four concentrations (0, 25, 50 and 100 mg L-1) of MWCNTs-COOH under three salt stress levels (0, 50 and 100 mM NaCl). An array of agronomic, physiological, analytical and biochemical parameters were evaluated in an attempt to examine the potential use of MWCNTs in plants under optimal and abiotic stress conditions. Application of MWCNTs-COOH at optimum concentration (50 mg L-1) could ameliorate the negative effects of salinity stress by increasing chlorophyll and carotenoids content and inducing non-enzymatic (i.e. phenolic content) and enzymatic antioxidant components (i.e. ascorbate peroxidase (APX), catalase (CAT) and guaiacol peroxidase (GP) activity). Furthermore, MWCNTs-COOH treatments under optimal conditions induced plant growth, while a significant increase (P ≤ 0.01) was recorded in essential oil content and compound profile. On the other hand, biochemical and epifluorescence microscopy evidence suggested that high dosage (100 mg L-1) of MWCNTs-COOH leads to toxicity effects in plant tissue. Overall, the positive response of plants to low concentrations of MWCNTs-COOH under control and abiotic stress conditions renders them as potential novel plant growth promoting and stress protecting agents, opening up new perspectives for their use in agriculture.

Interaction between hydrogen peroxide and sodium nitroprusside following chemical priming of Ocimum basilicum L. against salt stress.

Sodium nitroprusside (SNP) and hydrogen peroxide (H2 O2 ), as priming agents, have the well-recorded property to increase plant tolerance against a range of different abiotic stresses such as salinity. In this regard, the present study was conducted to evaluate the effect of different levels of SNP (100 and 200 µM) and H2 O2 (2.5 and 5 mM) as well as their combinations under salt stress (0 and 50 mM NaCl) on key physiological and biochemical attributes of the economically important aromatic plant basil (Ocimum basilicum L.) grown under hydroponic culture. Results revealed that morphological parameters such as plant height, root length, leaf fresh and dry weights (FW and DW) were significantly decreased by salinity stress, while SNP and H2 O2 treatments, alone or combined, increased FW and DW thus enhancing plant tolerance to salt stress. Furthermore, 200 µM SNP + 2.5 mM H2 O2 appeared to be the most effective treatment by causing significant increase in chlorophyll a and b, anthocyanin content and guaiacol peroxidase and ascorbate peroxidase enzymes activities under saline condition. In addition, analytical measurements showed that essential oil profile (concentration of main components) under salt stress was mostly affected by SNP and H2 O2 treatments. The highest increase was observed for methyl chavicol (43.09-69.91%), linalool (4.8-17.9%), cadinol (1.5-3.2%) and epi-α-cadinol (0.18-10.75%) compounds. In conclusion, current findings demonstrated a positive crosstalk between SNP and H2 O2 toward improved basil plant tolerance to salt stress, linked with regulation of essential oil composition.

Pre-véraison treatment of salicylic acid to enhance anthocyanin content of grape (Vitis vinifera L.) berries.

BACKGROUND: Grape berries produce significant amounts of phenolic compounds. These are an essential qualitative factor due to their nutritional value and effect on berry color and texture. Salicylic acid (SA) and its derivatives usually lead to enhancement of phenolic content in plant tissues. The present study was conducted to evaluate the effect of different levels of SA (0.0, 50.0, 100.0, and 200.0 mM) on the production of phenolic compounds and the derivatives (anthocyanin and flavonoid) in the grape berries, with emphasis on malvidin-3-O-ß glucoside as a regular anthocyanin in red grapes. RESULT: The results showed that total phenolics content were significantly enhanced in SA-treated (100.0 and 200.0 mM) berries compared to untreated ones. Salicylic acid treatment at all concentrations considerably improved the anthocyanin content in the berries and, compared with untreated berries, the accumulation of malvidin-3-O-ß glucoside was higher in SA-treated fruits. In particular, the 200.0 mM concentration caused approximately two times more malvidin-3-O-ß glucoside than the control. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging capacity of the fruits treated with SA were significantly higher than those of the untreated berries. The activity of phenylalanine ammonia-lyase (PAL) in SA treated fruits significantly increased as compared with the untreated clusters. CONCLUSION: A general evaluation of the current results leads us to the conclusion that SA is a suitable and recommendable treatment for improving and increasing the phenolic and antioxidant capacity of grape berries. Spraying grape berries at pre-véraison stage with SA could therefore be a convenient strategy to increase quality and nutritional value of grape berries considerably. © 2019 Society of Chemical Industry.

Toxicological implications of selenium nanoparticles with different coatings along with Se4+ on Lemna minor.

Nanoparticles have potential high risks for living organisms in the environment due to their specific qualities and their easy access. In the present study, selenium nanoparticles (Se NPs) with two different coatings (l-cysteine and tannic acid) were synthesized. The characteristics of particles were analyzed using XRD, FT-IR and SEM. The impact of the nanoparticles besides Se4+, on the aquatic higher plant Lemna minor was evaluated and compared. Entrance of l-cysteine and tannic acid capped Se NPs in the roots of Lemna minor was proved by TEM and fluorescence microscopy. Adverse effects of mentioned NPs and differences of these effects from those by sodium selenite as the ionic form were assessed by a range of biophysicochemical tests. Altogether, the results asserted that Lemna minor was notably poisoned by both capped Se NPs and Se4+. Thus, growth and photosynthetic pigments were decreased while lipid peroxidation along with total phenol and flavonoid contents were raised. Eventually some changes in enzymatic activities were presented. To sum up the consequences, it can be concluded that all changes occurred due to the plant defense system especially in order to remove reactive oxygen species (ROS) and possible phytotoxicity originated by l- cysteine and tannic acid capped Se NPs in addition to Se4+. The influence of tannic acid capped Se NPs after sodium selenite is stronger by the means of antioxidant enzymes activity in comparison with l-cysteine capped Se NPs.