Characterization of ruminal temperature and its relationship with ruminal pH in beef heifers fed growing and finishing diets.

This study characterized the relationship between ruminal temperature (T rum) and pH in beef cattle fed growing and finishing diets. In Exp. 1, 16 ruminally cannulated beef heifers (388.5 ± 34.9 kg BW) were fed 4 growing diets in a replicated 4 × 4 Latin square with four 21-d periods. Diets were (DM basis) grower control (CTLg; 35% barley grain plus 5% canola meal), 40% corn dried distillers grains plus solubles (CDDGSg), 40% wheat dried distillers grains plus solubles (WDDGSg), and 37.6% WDDGSg plus 2.4% corn oil (WDDGSg+O). All diets contained 55% barley silage (DM basis). Ruminal pH and T rum were continuously monitored for 4 d each period starting on d 18. In Exp. 2, the 16 heifers were gradually transitioned (529.1 ± 41.1 kg BW) from the growing diets to 1 of 4 finishing diets in a replicated 4 × 4 Latin square with four 28-d periods. Diets were (DM basis) finisher control (CTL f), 40% corn-based distillers grains plus solubles (CDDGS f), 40% wheat-based distillers grains plus solubles (WDDGS f), and 37.4% WDDGSf + 2.6% corn oil (WDDGS f+O). All finishing diets contained 8% barley silage (DM basis). Ruminal pH and T rum were measured from d 25 to 28. With growing diets (n = 64), maximum T rum was negatively related to minimum pH (P < 0.001, r = -0.53) and positively related to starch intake (P < 0.001, r = 0.55). Maximum T rum, T rum > 40°C (h/d), and area under the curve (AUC) T rum > 38°C (area × h/d) accounted for 28.3, 9.5, and 4.7%, respectively, of the variability in minimum pH (R(2) = 0.43, P < 0.001, n = 64). Mean T rum and maximum T rum were greater for CTL g than CDDGS g, WDDGS g, and WDDGS g+O. With finishing diets (n = 63), maximum T rum was negatively related to minimum ruminal pH (P < 0.001, r = -0.63) and positively related to DMI (P < 0.001, r = 0.62) and to starch intake (P < 0.001, r = 0.58). Maximum T rum, AUC T rum > 39°C, and duration T rum > 38°C accounted for 40, 17, and 3.6%, respectively, of the variability in minimum pH (R(2) = 0.60, P < 0.001, n = 63). Mean T rum and maximum T rum were greater for CTL f than CDDGS f, WDDGS f, and WDDGS f+O. When individual animal data were examined, heifers with duration T rum > 40°C did not necessarily have greater duration pH < 5.2 or pH < 5.5. Ruminal temperature has the potential to predict ruminal pH, likely owing to the biological relationship between acid production and the heat of fermentation. Exploitation of this relationship to predict pH could provide a means of overcoming the problems associated with long-term monitoring of ruminal pH using electrode-based approaches.

Effect of dried distillers grains plus solubles on enteric methane emissions and nitrogen excretion from growing beef cattle.

The objectives of this study were to examine the impact of corn- or wheat-based dried distillers grains with solubles (CDDGS or WDDGS) on enteric methane (CH4) emissions from growing beef cattle and determine if the oil in CDDGS was responsible for any response observed. Effects of CDDGS or WDDGS on total N excretion and partitioning between urine and fecal N were also examined in this replicated 4 × 4 Latin square using 16 ruminally cannulated crossbreed heifers (388.5 ± 34.9 kg of initial BW). The control diet contained (DM basis) 55% whole crop barley silage, 35% barley grain, 5% canola meal, and 5% vitamin and mineral supplement. Three dried distillers grains with solubles (DDGS) diets were formulated by replacing barley grain and canola meal (40% of dietary DM) with CDDGS, WDDGS, or WDDGS plus corn oil (WDDGS+oil). For WDDGS+oil, corn oil was added to WDDGS (4.11% fat DM basis) to achieve the same fat level as in CDDGS (9.95% fat DM basis). All total mixed diets were fed once daily ad libitum. Total collection of urine and feces was conducted between d 11 and 14. Enteric CH4 was measured between d 18 and 21 using 4 environmental chambers (2 animals fed the same diet per chamber). Methane emissions per kilogram of DM intake (DMI) and as percent of GE intake (GEI) among heifers fed WDDGS (23.9 g/kg DMI and 7.3% of GEI) and the control (25.3 g/kg DMI and 7.8% of GEI) were similar (P = 0.21 and P = 0.19) whereas heifers fed CDDGS (21.5 g/kg DMI and 6.6% of GEI) and WDDGS+oil (21.1 g/kg DMI and 6.3% of GEI) produced less (P < 0.05) CH4. Total N excretion (g/d) differed (P < 0.001) among treatments with WDDGS resulting in the greatest total N excretion (303 g/d) followed by WDDGS+oil (259 g/d), CDDGS (206 g/d), and the control diet (170 g/d), respectively. Compared with the control diet, heifers offered WDDGS, CDDGS, and WDDGS+oil excreted less fecal N (P < 0.001) but more (P < 0.001) urinary N. Results suggest that high-fat CDDGS or WDDGS+oil can mitigate enteric CH4 emissions in growing beef cattle. However, to completely assess the impact of DDGS on greenhouse gas emissions of growing feedlot cattle, the potential contribution of increased N excretion to heightened NH3 and nitrous oxide emissions requires consideration.