Highly sensitive qualitative and quantitative detection of saturated fatty aldehydes in edible vegetable oils using a "turn-on" fluorescent probe by high performance liquid chromatography.

A new turn-on fluorescent probe, based on a hydrazine group placed in the meso-position of the BODIPY molecule, was synthesized. It was then used for detecting long-chain fatty aldehydes, which can be harmful to human health, in edible vegetable oils. In acetonitrile, the probe produced strong "turn on" and 100-fold fluorescence enhancement with high sensitivity and rapid response to saturated fatty aldehydes. A highly sensitive detection method for long-chain fatty aldehydes was established using pre-column derivation fluorescence procedure by high-performance liquid chromatography. The chromatographic method established provided satisfactory precision (1.91%-5.93%), good linearity (R2 ≥ 0.999), an acceptable accuracy (83.7%-108%) and a low limit of detection (6.4-12.4 ng/mL). The experimental results indicated that the probe could qualitatively and quantitatively detect six fatty aldehydes in vegetable oils, thus providing the potential for use in routine analysis for identifying the type of vegetable oil and for controlling its quality and safety.

MicroRNA-215 suppresses cell proliferation, migration and invasion of colon cancer by repressing Yin-Yang 1.

Colorectal cancer is one of the most common malignant tumors worldwide with rising incidence. MicroRNAs are small non-coding RNAs that implicate in multiple physiological or pathological processes. The aberrant expression of miRNA-215 (miR-215) has been illustrated in various types of cancers. However, the expression of miR-215 in human colon cancer and the biological roles of it remain largely unknown. We conducted this study to explore the expression and the function of miR-215 in human colon cancer. The results showed that miR-215 was remarkably downregulated in colon cancer tissues and cell lines. Overexpression of miR-215 by miR-215 mimic significantly inhibited colon cancer cell proliferation, migration and invasion while knockdown of miR-215 by miR-215 inhibitor exerted reverse effects. Furthermore, we newly identified Yin-Yang 1(YY1) as a direct target of miR-215 which could rescue the effects of miR-215 on colon cancer cells. In summary, our investigation revealed that miR-215 was downregulated in colon cancer and it suppressed colon cancer cell proliferation, migration and invasion by directly targeting YY1.