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1.
Cell Mol Biol (Noisy-le-grand) ; 68(8): 156-162, 2022 Aug 31.
Artículo en Inglés | MEDLINE | ID: mdl-36800816

RESUMEN

It was to investigate the breast milk cell immune function and the effect of health education on pregnant and lying-in women. 100 primiparas were randomly divided into the control group (50 cases): routine health education; the test group (50 cases): prenatal breastfeeding health education based on the control group. Breastfeeding status, as well as breast milk immune cell composition at each stage, were compared between the two groups after intervention. After the intervention, the maternal feeding knowledge score of the test group (17.3 ± 2.4) points was significantly higher than that of the control group (14.1 ± 2.9) points (P < 0.05); the total feeding self-efficacy score of the test group was significantly higher than that of the control group at four weeks after delivery and eight weeks after delivery (P < 0.05); at eight weeks after delivery, 42 parturients in test group chose exclusive breastfeeding, significantly more than 22 parturients in the control group (P < 0.05); during colostrum, CD3+ accounted for (57.8 ± 4.2)%, CD4+ accounted for (31.5 ± 3.7)%, CD8+ accounted for (26.2 ± 2.4)%, CD4+/CD8+ was (1.2 ± 0.3), significantly higher than those of transitional milk and mature milk (P < 0.05); during colostrum, IFN-γ was (1.4 ± 0.4) µg/L, IL-8 was (1.4 ± 0.4) µg/L, significantly higher than those of mature milk (P < 0.05). Breast milk is beneficial to improve the immune function of newborns. It is necessary to perform health education for pregnant and lying-in women and improve the breastfeeding rate.


Asunto(s)
Lactancia Materna , Leche Humana , Embarazo , Humanos , Recién Nacido , Femenino , Calostro , Educación en Salud , Inmunidad
2.
Biochem Biophys Res Commun ; 510(3): 472-478, 2019 03 12.
Artículo en Inglés | MEDLINE | ID: mdl-30737028

RESUMEN

Retroviral nucleocapsid (NC) proteins are multifunctional nucleic acid binding proteins, playing critical roles in essentially every step of the viral replication cycle. As a small, basic protein, NC contains one or two highly conserved zinc-finger domains, each having an invariant CCHC motif, flanked by basic residues. In this study, we report for the first time, to our knowledge, the thermostable property of equine infectious anemia virus (EIAV) NCp11. About 43% of purified NCp11 remained soluble after incubation at 100 °C for 60 min, and heat-treated NCp11 maintained its abilities to bind to the E. coli RNA and the EIAV packaging signal sequence. At a very high degree of sequence occupancy, NCp11 inhibited first-strand cDNA synthesis catalyzed by either a commercial or the purified EIAV reverse transcriptase, and heat-treated NCp11 still inhibited the first-strand cDNA synthesis. We also found that protein concentrations, at a range from 0.1 to 0.9 µg/µl, have not affected the NCp11 thermostability significantly. However, NCp11 at acidic pH was more thermostable. Our findings highlight a new feature of the NC protein. Detailed understanding of NC's properties and functions will facilitate the development of effective and rational therapeutic strategies against retroviruses.


Asunto(s)
Proteínas de la Nucleocápside/química , Proteínas de la Nucleocápside/metabolismo , ADN Complementario/biosíntesis , Ácido Edético , Calor , Concentración de Iones de Hidrógeno , Estabilidad Proteica , ARN/metabolismo
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