Initial Assessment of a Brief Health, Fitness, and Spirituality Survey for Epidemiological Research: A Pilot Study.

Background: Not many population-based health studies include items to assess both fitness and spirituality concepts. Therefore, the purpose of this study was to examine initial data of a brief health, fitness, and spirituality survey for epidemiological research. Methods: This first phase pilot study used data from N = 56 adults 18+ years of age via electronic questionnaire. Measures of general health, fitness, physical activity (PA), body mass index (BMI), religiosity, and happiness were assessed. Reliability analyses were employed for PA, religiosity, and happiness scales. Validity coefficients were computed to evaluate convergence between scale scores and related measures. Finally, difference in health was examined between different levels of fitness to evaluate known groups discrimination. Results: Respondents were middle-aged (Mean = 50.5 yr, SD = 14.3), majority white (69.5%, SD = 6.2), with relatively low BMI (Mean = 25.3, SD = 5.3). All three scales showed internal consistency reliability of α = 0.93, α = 0.89, and KR-20 = 0.56 for religiosity, happiness, and PA, respectively. Furthermore, scores converged (ps < 0.05) between fitness and PA (r = 0.43), health (r = 0.66), BMI (r = -0.28), and happiness (r = 0.25). Finally, health scores were significantly greater for high fitness versus low fitness in both male (p < 0.001) and female (p = 0.015) populations. Conclusion: Results from this study indicate that a brief health, fitness, and spirituality survey can reliably measure its intended traits. A single-item of self-assessed fitness in particular has promise for large scale epidemiological research.

Immunization With Mycobacterium tuberculosis Antigens Encapsulated in Phosphatidylserine Liposomes Improves Protection Afforded by BCG.

Liposomes have been long considered as a vaccine delivery system but this technology remains to be fully utilized. Here, we describe a novel liposome-based subunit vaccine formulation for tuberculosis (TB) based on phosphatidylserine encapsulating two prominent TB antigens, Ag85B, and ESAT-6. We show that the resulting liposomes (Lipo-AE) are stable upon storage and can be readily taken up by antigen presenting cells and that their antigenic cargo is delivered and processed within endosomal cell compartments. The Lipo-AE vaccine formulation combined with the PolyIC adjuvant induced a mixed Th1/Th17-Th2 immune response to Ag85B but only a weak response to ESAT-6. An immunization regimen based on systemic delivery followed by mucosal boost with Lipo-AE resulted in the accumulation of resident memory T cells in the lungs. Most importantly though, when Lipo-AE vaccine candidate was administered to BCG-immunized mice subsequently challenged with low dose aerosol Mycobacterium tuberculosis, we observed a significant reduction of the bacterial load in the lungs and spleen compared to BCG alone. We therefore conclude that the immunization with mycobacterial antigens delivered by phosphatidylserine based liposomes in combination with Poly:IC adjuvant may represent a novel BCG boosting vaccination strategy.

Experimental Models of Anxiety for Drug Discovery and Brain Research.

Animal models have been vital to recent advances in experimental neuroscience, including the modeling of common human brain disorders such as anxiety, depression, and schizophrenia. As mice express robust anxiety-like behaviors when exposed to stressors (e.g., novelty, bright light, or social confrontation), these phenotypes have clear utility in testing the effects of psychotropic drugs. Of specific interest is the extent to which mouse models can be used for the screening of new anxiolytic drugs and verification of their possible applications in humans. To address this problem, the present chapter will review different experimental models of mouse anxiety and discuss their utility for testing anxiolytic and anxiogenic drugs. Detailed protocols will be provided for these paradigms, and possible confounds will be addressed accordingly.

Experimental models of anxiety for drug discovery and brain research.

Animal models have been vital to recent advances in experimental neuroscience, including the modeling of common human brain disorders such as anxiety, depression, and schizophrenia. As mice express robust anxiety-like behaviors when exposed to stressors (e.g., novelty, bright light, or social confrontation), these phenotypes have clear utility in testing the effects of psychotropic drugs. Of specific interest is the extent to which mouse models can be used for the screening of new anxiolytic drugs and verification of their possible applications in humans. To address this problem, the present chapter will review different experimental models of mouse anxiety and discuss their utility for testing anxiolytic and anxiogenic drugs. Detailed protocols will be provided for these paradigms, and possible confounds will be addressed accordingly.