RESUMEN
No abstract available.
Asunto(s)
Humanos , Masculino , Persona de Mediana Edad , Terapia por Acupuntura , Ampicilina/farmacología , Tobillo/diagnóstico por imagen , Antibacterianos/farmacología , Celulitis (Flemón)/complicaciones , Diabetes Mellitus Tipo 2/complicaciones , Infecciones por Bacterias Gramnegativas/complicaciones , Hipertensión/complicaciones , Imagen por Resonancia Magnética , Pruebas de Sensibilidad Microbiana , Prevotella nigrescens/efectos de los fármacos , ARN Ribosómico 16S/análisis , Sulbactam/farmacología , Tomografía Computarizada por Rayos XRESUMEN
BACKGROUND: CELL-DYN Sapphire (Abbott Diagnostics, Santa Clara, CA, USA) hematology analyzer is based on laser multi angle polarized scatter separation, focused flow impedance and fluorescent flow cytometry. In order to assess the usefulness of this newly introduced analyzer, we evaluated the precision and correlation of complete blood count including white blood cell differential count and reticulocyte count of CELL-DYN Sapphire instrument. METHODS: Patient samples ordered for complete blood count and white blood cell differential count and CELL-DYN 29 Plus control samples were used. We evaluated the precision of complete blood count and WBC differential count and analyzed correlation of these parameters for comparison with ADVIA 120 (Bayer corporation, Tarrytown, NY, USA) and manual count. We additionally evaluated reticulocyte parameters for precision and comparison with ADVIA 120. RESULTS: Short term precisions showed low coefficient of variation (CV) values for all CBC parameters including lower than 3% CV values for neutrophil and lymphocyte count. Long term precision showed lower than 4% CV values for all CBC and WBC differential count except for basophils. Comparison with ADVIA 120 showed good correlation for CBC except for MCHC. Good correlation was confirmed in all differential counts except for basophils. Comparison with manual WBC differential count showed good correlation except for monocyte and eosinophil. CONCLUSIONS: CELL-DYN Sapphire has high precision and good correlation with ADVIA 120 and manual count. CELL-DYN Sapphire is an exceptional instrument for CBC with WBC differentials and reticulocyte counting.
Asunto(s)
Humanos , Óxido de Aluminio , Basófilos , Recuento de Células Sanguíneas , Impedancia Eléctrica , Citometría de Flujo , Hematología , Leucocitos , Recuento de Linfocitos , Monocitos , Neutrófilos , Recuento de Reticulocitos , ReticulocitosRESUMEN
BACKGROUND: CELL-DYN Sapphire (Abbott Diagnostics, Santa Clara, CA, USA) hematology analyzer is based on laser multi angle polarized scatter separation, focused flow impedance and fluorescent flow cytometry. In order to assess the usefulness of this newly introduced analyzer, we evaluated the precision and correlation of complete blood count including white blood cell differential count and reticulocyte count of CELL-DYN Sapphire instrument. METHODS: Patient samples ordered for complete blood count and white blood cell differential count and CELL-DYN 29 Plus control samples were used. We evaluated the precision of complete blood count and WBC differential count and analyzed correlation of these parameters for comparison with ADVIA 120 (Bayer corporation, Tarrytown, NY, USA) and manual count. We additionally evaluated reticulocyte parameters for precision and comparison with ADVIA 120. RESULTS: Short term precisions showed low coefficient of variation (CV) values for all CBC parameters including lower than 3% CV values for neutrophil and lymphocyte count. Long term precision showed lower than 4% CV values for all CBC and WBC differential count except for basophils. Comparison with ADVIA 120 showed good correlation for CBC except for MCHC. Good correlation was confirmed in all differential counts except for basophils. Comparison with manual WBC differential count showed good correlation except for monocyte and eosinophil. CONCLUSIONS: CELL-DYN Sapphire has high precision and good correlation with ADVIA 120 and manual count. CELL-DYN Sapphire is an exceptional instrument for CBC with WBC differentials and reticulocyte counting.
Asunto(s)
Humanos , Óxido de Aluminio , Basófilos , Recuento de Células Sanguíneas , Impedancia Eléctrica , Citometría de Flujo , Hematología , Leucocitos , Recuento de Linfocitos , Monocitos , Neutrófilos , Recuento de Reticulocitos , ReticulocitosRESUMEN
Pasteurella multocida infection in man is rare. This organism was isolated from a calf infection of a 48-year-old woman with degenerative arthritis who had the history of treatments with prednisolone, acupuncture and moxa cauterization. She did not have any animal contact. It was condsidered probable that the organism invaded through the cauterization ulcers. The organism was difficult to identify, because of its superficial resemblance to other organisms. Oxi/Ferm and N/F systems failed to identify the isolate. The organism was susceptible to many antimicrobial agents tested except to amikacin and clindamycin.