[A study on AFLP fingerprinting of land races of Panax ginseng L].

OBJECTIVE: To obtain more information on DNA fingerprintings of five land races of Chinese ginseng, namely, Damaya (DMY), Changbo (CB), Yuanbangyuanlu (YBYL) and Huangguo (HG). METHODS: The five land races were detected by amplified restriction fragment polymorphism (AFLP) markers with 11 combined primers (M2, M3, M16, M20, M53, M56, M57, M68, M69, M72, M84 in Mse I). RESULT AND CONCLUSION: Only 4.6% polymorphic sites was found. It was further verified that only a little diversity existed among the land races. The polymorphic sites of CB were much more than those of the others, which suggests that there are more heterozygotes in CB populations, and it is closer to wild ginseng than the others.

[Comparison of ITS sequences between wild ginseng DNA and garden ginseng DNA].

OBJECTIVE: To compare the genetic differences between wild ginseng and garden ginseng (Panax ginseng). METHOD: The sequences of ITS1 and ITS2 of wild ginsengs were determined on LKB DNA sequencing station through Si-liver Sequence DNA Sequencing System. The sequencies were aligned with DNA SIS software. RESULTS AND CONCLUSION: The ITS1 and ITS2 of Panax were 220-221 and 222-224 bases in length respectively. In Panax ginsehg, the seqences of ITS1 were very stable, but ITS2 were changeable. The ITS2 sequences of No. 87 and No. 110 of the wild ginseng collected from Fusong Heilongjiang (China) were exactly the same as those of No. U41680(Jun Wen) and No. U41682(Jun Wen) of garden ginseng collected from Heilongjiang Province (China) and Korea respectively, but different from those of No. U41681(Jun Wen) from Hubei Province (China) in three bases (447, 449, 450) The result implies that the cultivated ginsengs may have been introduced from two different populations of the wild ginseng.