RESUMEN
To monitor the biological effects of marine pollution, choosing a native fish species and establishing suitable biomarkers are required. In this study, the full-length cDNA of cyp1a1 was cloned from Sebastiscus marmoratus (SM-CYP1A1). Then the dose-response and time-course induction of hepatic CYP1A1 mRNA by the crude oil water-soluble fraction (WSF) were determined. Subsequently, SM-CYP1A1 mRNA was applied to investigate the biological effect of petroleum hydrocarbon pollution in Quanzhou Bay, China. The transcription levels of hepatic CYP1A1 were significantly elevated in fish caged in the polluted sites for 2weeks compared with those of the reference site, which were correlated with the concentrations of petroleum hydrocarbon and polycyclic aromatic hydrocarbon (PAH) in the surface seawaters. The results suggest that S. marmoratus is a potential sentinel organism to monitor marine pollutants and the hepatic CYP1A1 mRNA can serve as a sensitive biomarker to organic xenobiotics in aquatic environments.
Asunto(s)
Exposición a Riesgos Ambientales , Peces/genética , Peces/metabolismo , Petróleo/efectos adversos , Hidrocarburos Policíclicos Aromáticos/efectos adversos , Contaminantes Químicos del Agua/efectos adversos , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Bahías , China , Citocromo P-450 CYP1A1/genética , Citocromo P-450 CYP1A1/metabolismo , Relación Dosis-Respuesta a Droga , Monitoreo del Ambiente , Proteínas de Peces/genética , Proteínas de Peces/metabolismo , Perfilación de la Expresión Génica/veterinaria , Contaminación por Petróleo , Filogenia , Especies Centinela/genética , Especies Centinela/metabolismo , Alineación de Secuencia/veterinariaRESUMEN
The aim of this study was to characterize dose- and time-dependent responses of gill 7-ethoxyresorufin O-deethylase (EROD) activity from Juvenile marbled rockfish (Sebastiscus marmoratus) exposed to the water-accommodated fraction (WAF) of crude oil and heavy metal Cd(â ¡) or Pb(â ¡) alone or in mixture. Compared to the control group, gill filament EROD activity in S. marmoratus was significantly induced after exposure to the WAF from 80 to 320µg/L for 5 days in dose response experiment and after exposure to 40µg/L WAF for 6-10 days in time course experiment, respectively. In the other hand, gill filament EROD activity were not significantly affected compared to the control group or related WAF groups no matter in the dose response experiment or in the time course experiment of Cd(â ¡), Pb(â ¡) or its mixture with WAF. The results suggest the use of gill filament EROD activity as a biomarker of exposure to waterborne AhR agonists in marine ecosystems while simultaneously being exposed to environmental concentrations of Cd(â ¡) or Pb(â ¡).