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Métodos Terapéuticos y Terapias MTCI
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PLoS One ; 9(11): e112093, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-25391152

RESUMEN

Alkaloid-containing natural compounds have shown promise in the treatment of microbial infections. However, practical application of many of these compounds is pending a mechanistic understanding of their mode of action. We investigated the effect of two alkaloids, piperine (found in black pepper) and reserpine (found in Indian snakeroot), on the ability of the uropathogenic bacterium Escherichia coli CFT073 to colonize abiotic surfaces. Sub-inhibitory concentrations of both compounds (0.5 to 10 µg/mL) decreased bacterial swarming and swimming motilities and increased biofilm formation. qRT-PCR revealed a decrease in the expression of the flagellar gene (fliC) and motility genes (motA and motB) along with an increased expression of adhesin genes (fimA, papA, uvrY). Interestingly, piperine increased penetration of the antibiotics ciprofloxacin and azithromycin into E. coli CFT073 biofilms and consequently enhanced the ability of these antibiotics to disperse pre-established biofilms. The findings suggest that these alkaloids can potentially affect bacterial colonization by hampering bacterial motility and may aid in the treatment of infection by increasing antibiotic penetration in biofilms.


Asunto(s)
Alcaloides/uso terapéutico , Antibacterianos/uso terapéutico , Biopelículas/efectos de los fármacos , Regulación Bacteriana de la Expresión Génica/efectos de los fármacos , Escherichia coli Uropatógena/efectos de los fármacos , Adhesinas Bacterianas/metabolismo , Alcaloides/química , Adhesión Bacteriana/efectos de los fármacos , Benzodioxoles/uso terapéutico , Proteínas de Escherichia coli/metabolismo , Flagelos/metabolismo , Flagelina , Piperidinas/uso terapéutico , Extractos Vegetales/química , Alcamidas Poliinsaturadas/uso terapéutico , Reserpina/uso terapéutico
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