RESUMEN
Selenium-doped nanostructure has been considered as an attractive approach to enhance the antibacterial activity of calcium phosphate (CaP) materials in diverse medical applications. In this study, the selenium-doped biphasic calcium phosphate nanoparticles (SeB-NPs) were first synthesized. Then, silver was in situ incorporated into SeB-NPs to obtain nanostructured composite nanoparticles (AgSeB-NPs). Both SeB-NPs and AgSeB-NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV-Vis), X-ray photoelectron spectroscopy (XPS), and Raman spectra. The results confirmed that the SeO32- was doped at the PO43- position and silver nanoparticles were deposited on the surface of SeB-NPs. Next, Transmission Electron Microscopy (TEM) analysis displayed that the prepared AgSeB-NPs had a needle-cluster-like morphology. CCK-8 analysis revealed SeB-NPs and AgSeB-NPs had good cytocompatibility with osteoblasts. The antibacterial activity of the prepared AgSeB-NPs was confirmed by using Gram-negative E. coli and Gram-positive S. aureus. The above results manifested the significance of the final AgSeB-NPs for biomedical applications.
Asunto(s)
Antibacterianos/farmacología , Hidroxiapatitas/química , Hidroxiapatitas/farmacología , Nanopartículas del Metal/química , Nanoestructuras/química , Selenio/química , Células Cultivadas , Escherichia coli/efectos de los fármacos , Humanos , Pruebas de Sensibilidad Microbiana/métodos , Microscopía Electrónica de Transmisión/métodos , Tamaño de la Partícula , Espectroscopía de Fotoelectrones/métodos , Plata/química , Espectrofotometría Ultravioleta/métodos , Espectroscopía Infrarroja por Transformada de Fourier/métodos , Staphylococcus aureus/efectos de los fármacos , Difracción de Rayos X/métodosRESUMEN
Radiation crosslinked ultra high molecular weight polyethylene (UHMWPE) with high mechanical strength, wear resistance, and oxidative stability is critical for the long term performance and life span of the joint implants based on crosslinked UHMWPE. The use of antioxidants and/or radical scavengers has proven efficient in stabilising crosslinked UHMWPE against oxidation, whereas challenges remain to explore new methods to offer not only oxidative stability, but also superior wear resistance and mechanical properties in the long term. Herein, two natural polyphenols, gallic acid (GA) and dodecyl gallate (DG), containing three hydroxy groups each, have been demonstrated potent in protecting irradiated UHMWPE against adverse oxidative challenging at elevated temperatures. The presence of these polyphenols does not inhibit the crosslinking in comparison to the high dose-irradiated vitamin E/UHMWPE. The tensile and impact properties of these polyphenol-stabilized highly cross-linked UHMWPE after accelerated ageing in accordance with ASTM F2003 are superior to those of the irradiated and remelted UHMWPE. Pin-on-disc (POD) wear tests of these materials demonstrated low wear compared to that of highly crosslinked and remelted UHMWPE. In vitro culture with chondrocytes showed a slightly improved biocompatibility for these irradiated antioxidant/UHMWPE. These natural polyphenols are potential alternatives for the stabilisation of highly crosslinked UHMWPE for joint arthroplasty.