RESUMEN
Plant-based oral vaccines are a promising emergent technology that could help alleviate disease burden worldwide by providing a low-cost, heat-stable, oral alternative to parenterally administered commercial vaccines. Here, we describe high-level accumulation of the hepatitis B surface antigen (HBsAg) at a mean concentration of 0.51%TSP in maize T1 seeds using an improved version of the globulin1 promoter. This concentration is more than fourfold higher than any previously reported lines. HBsAg expressed in maize seeds was extremely heat stable, tolerating temperatures up to 55 °C for 1 month without degradation. Optimal heat stability was achieved after oil extraction of ground maize material, either by supercritical fluid extraction or hexane treatment. The contributions of this material towards the development of a practical oral vaccine delivery system are discussed.
Asunto(s)
Antígenos de Superficie de la Hepatitis B/biosíntesis , Vacunas contra Hepatitis B/biosíntesis , Plantas Modificadas Genéticamente/metabolismo , Semillas/química , Zea mays/inmunología , Antígenos de Superficie de la Hepatitis B/análisis , Extractos Vegetales/análisis , Extractos Vegetales/inmunología , Aceites de Plantas/análisisRESUMEN
The benefits of using plant-based oral vaccines are discussed. Transgenic maize expressing an antigen of transmissible gastroenteritis virus (TGEV) is reported as a model to demonstrate efficacy. Young pigs that were fed the TGEV corn orally were protected against challenge with virulent TGEV. Additional parameters important in providing a reliable and consistent supply of plant-based vaccines are discussed. Finally, vaccines developed in maize are evaluated for their potential to contaminate either the food supply or the environment.
Asunto(s)
Gastroenteritis Porcina Transmisible/prevención & control , Fitoterapia , Virus de la Gastroenteritis Transmisible/inmunología , Vacunación/veterinaria , Vacunas Virales , Zea mays , Animales , Comercio , Proyectos de Investigación , Porcinos , Estados UnidosRESUMEN
Plant systems are reviewed with regard to their ability to express and produce subunit vaccines. Examples of different types of expression systems producing a variety of vaccine candidates are illustrated. Many of these subunit vaccines have been purified and shown to elicit an immune response when injected into animal models. This review also includes vaccines that have been administered orally in a non-purified form as a food or feed product. Cases are highlighted which demonstrate that orally delivered plant-based vaccines can elicit immune responses and in some case studies, confer protection. Examples are used to illustrate some of the inherent advantages of a plant-based system, such as cost, ease of scale-up and convenience of delivery. Also, some of the key steps are identified that will be necessary to bring these new vaccines to the market.
Asunto(s)
Plantas/inmunología , Vacunas Sintéticas/inmunología , Administración Oral , Animales , Antígenos/administración & dosificación , Antígenos/inmunología , Cadena Alimentaria , Humanos , Tolerancia Inmunológica/inmunología , Inmunidad/inmunología , Extractos Vegetales/inmunología , Plantas Modificadas Genéticamente/inmunología , Vacunación/métodos , Vacunas de Subunidad/administración & dosificación , Vacunas de Subunidad/efectos adversos , Vacunas de Subunidad/inmunología , Vacunas Sintéticas/administración & dosificación , Vacunas Sintéticas/efectos adversosRESUMEN
The use of recombinant gene technologies by the vaccine industry has revolutionized the way antigens are generated, and has provided safer, more effective means of protecting animals and humans against bacterial and viral pathogens. Viral and bacterial antigens for recombinant subunit vaccines have been produced in a variety of organisms. Transgenic plants are now recognized as legitimate sources for these proteins, especially in the developing area of oral vaccines, because antigens have been shown to be correctly processed in plants into forms that elicit immune responses when fed to animals or humans. Antigens expressed in maize (Zea mays) are particularly attractive since they can be deposited in the natural storage vessel, the corn seed, and can be conveniently delivered to any organism that consumes grain. We have previously demonstrated high level expression of the B-subunit of Escherichia coli heat-labile enterotoxin and the spike protein of swine transmissible gastroenteritis in corn, and have demonstrated that these antigens delivered in the seed elicit protective immune responses. Here we provide additional data to support the potency, efficacy, and stability of recombinant subunit vaccines delivered in maize seed.