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1.
Nutr Cancer ; 67(2): 275-84, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-25622112

RESUMEN

Phellinus linteus (PL) is a medicinal mushroom due to its several biological properties, including anticancer activity. However, the mechanisms of its anticancer effect remain to be elucidated. We evaluated the inhibitory effects of the ethanolic extract from the PL combined with 5-FU on MDA-MB-231 breast cancer cell line and to determine the mechanism of cell death. Individually, PL extract and 5-FU significantly inhibited the proliferation of MDA-MB-231 cells in a dose-dependent manner. PL extract (30 mg/mL) in combination with 5-FU (10 µg/mL) synergistically inhibited MDA-MB-231 cells by 1.8-fold. PL did not induce apoptosis, as demonstrated by the DNA fragmentation assay, the sub-G1 population, and staining with annexin V-FITC and propidium iodide. The exposure of MDA-MB-231 cells to PL extracts resulted in several confirmed characteristics of autophagy, including the appearance of autophagic vacuoles revealed by monodansylcadaverine staining, the formation of acidic vesicular organelles, autophagosome membrane association of microtubule-associated protein light chain 3 (LC3) characterized by cleavage of LC3 and its punctuate redistribution, and ultrastructural observation of autophagic vacuoles by transmission electron microscopy. We concluded that PL extracts synergized with low doses of 5-FU to inhibit triple-negative breast cancer cell growth and demonstrated that PL extract can induce autophagy-related cell death.


Asunto(s)
Antineoplásicos/farmacología , Autofagia/efectos de los fármacos , Fluorouracilo/farmacología , Polisacáridos/farmacología , Neoplasias de la Mama Triple Negativas/tratamiento farmacológico , Agaricales/química , Protocolos de Quimioterapia Combinada Antineoplásica/farmacología , Apoptosis/efectos de los fármacos , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Sinergismo Farmacológico , Humanos , Proteínas Asociadas a Microtúbulos/efectos de los fármacos , Phellinus , Extractos Vegetales , Neoplasias de la Mama Triple Negativas/patología
2.
J Agric Food Chem ; 57(18): 8266-73, 2009 Sep 23.
Artículo en Inglés | MEDLINE | ID: mdl-19711916

RESUMEN

Licorice is a common Chinese medicinal herb with antitumor activity. Some components in licorice root have been shown to induce cell cycle arrest or apoptosis in cancer cells. This paper demonstrates for the first time that licorice Glycyrrhiza glabra and its component licochalcone-A (LA) can induce autophagy in addition to apoptosis in human LNCaP prostate cancer cells. Exposure of cells to licorice or LA resulted in several confirmed characteristics of autophagy, including the appearance of autophagic vacuoles revealed by monodansylcadaverine (MDC) staining, formation of acidic vesicular organelles (AVOs), and autophagosome membrane association of microtubule-associated protein 1 light chain 3 (LC3) characterized by cleavage of LC3 and its punctuate redistribution, as well as ultrastructural observation of autophagic vacuoles by transmission electron microscopy. Autophagy induction was accompanied by down-regulation of Bcl-2 and inhibition of the mammalian target of rapamycin (mTOR) pathway. In summary, licorice can induce caspase-dependent and autophagy-related cell death in LNCaP cells.


Asunto(s)
Autofagia/efectos de los fármacos , Chalconas/farmacología , Genes bcl-2/efectos de los fármacos , Glycyrrhiza/química , Neoplasias de la Próstata/ultraestructura , Proteínas Quinasas/efectos de los fármacos , Línea Celular Tumoral , Expresión Génica/efectos de los fármacos , Humanos , Masculino , Microscopía Electrónica de Transmisión , Neoplasias de la Próstata/metabolismo , Serina-Treonina Quinasas TOR , Vacuolas/ultraestructura
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