RESUMEN
AIMS: Phosphorus (P) is a finite resource and inoculation of phosphorus-mobilizing bacteria (PMB) is a promising approach for the enhancement of soil P availability and plant P uptake. This drives scientists to search for the microbes effective in mobilizing legacy P in soils. METHODS AND RESULTS: The current incubation and greenhouse pot experiments were conducted to investigate P mobilization and pepper P uptake as affected by a new biocontrol and bioremediation bacterium Burkholderia cepacia CQ18. This bacterium converted Ca3 (PO4 )2 , FePO4 , AlPO4 , and lecithin into soluble inorganic P in the culture solutions and increased available P (including water-soluble P and Olsen P) in the soil. There were positive correlations between the soluble inorganic phosphorus and the exudates (protons, organic acids (oxalate and gluconate), siderophores and phosphatases) in culture solutions. Pepper plant biomass, fruit yield and P uptake changed in the sequence: chemical fertilizers plus bacterial inoculant >only chemical fertilizers >only bacterial inoculant >blank control. CONCLUSIONS: Taking into account the wide spectrums of P mobilization and simultaneous production of acid, neutral and alkaline phosphatases at a given pH, B.cepacia CQ18 may be a potential PMB used in soils with wide pH ranges. The mechanisms employed by this bacterium in the solubilization of recalcitrant inorganic P could be the efflux of protons, organic acids (oxalate and gluconate) and siderophores. Phosphatases could be of utmost importance in the mineralization of the organic P. The production of siderophores and phosphatases by of B.cepacia CQ18 could thus be crucial for not only the antagonism against plant pathogens but also the mobilization of soil sparingly available P. SIGNIFICANCE AND IMPACT OF THE STUDY: Burkholderia cepacia CQ18 could be potentially developed into a biofertilizer.
Asunto(s)
Inoculantes Agrícolas/metabolismo , Burkholderia cepacia/metabolismo , Capsicum/metabolismo , Fósforo/farmacocinética , Biodegradación Ambiental , Disponibilidad Biológica , Capsicum/crecimiento & desarrollo , Fertilizantes/análisis , Fósforo/análisis , Exudados de Plantas/metabolismo , Suelo/química , Microbiología del SueloRESUMEN
The insecticidal activities of extracts from different parts of Cacalia tangutica (Franch.) H.-M. and two active ingredients, friedelin and stigmasterol, against Musca domestica L., Aedes albopictus (Skuse), and Spodoptera litura (F.) cells (SL cells) are reported. Extracts from leaves, flowers, and roots are highly active against adult M. domestica and the fourth-instar larvae of Ae. albopictus. The foliar extract was the most active with LC50 values of 1.74 mg/g and 196.94 microg/ml against adult M. domestica and fourth-instar larvae of Ae. albopictus, respectively. Extracts from stems were the least active. Friedelin and rotenone were highly active and equitoxic to these two insect species. The LC50 value of friedelin in adult M. domestica 48 h after treatment was 129.27 microg/g. The activity of stigmasterol on these two insect species was not obvious. However, the cytotoxicity of stigmasterol to S. litura cells was significantly greater than that of either friedelin or rotenone. At 25 microg/ml, the mortality of cells 24 h after treatment with stigmasterol was 23.0%. In contrast, friedelin and rotenone at the same concentration produced 16 and 18% mortality, respectively. Our results indicate differences between cytotoxicity in vitro and insect toxicity in vivo.
Asunto(s)
Aedes , Asteraceae/química , Moscas Domésticas , Insecticidas , Estigmasterol , Triterpenos , Aedes/crecimiento & desarrollo , Animales , Femenino , Larva , Masculino , Extractos Vegetales/químicaRESUMEN
A prospective study was made to estimate the effect of hyperbaric oxygen (HBO) on 14 eyes of cystoid macular edema (CME) secondary to retinal vein occlusion. The visual acuity was improved 2-6 lines (average 3.6 lines) after the treatment, while the control group of 12 cases showed little improvement. The therapeutic effect was stable, however, fluorescein angiography showed no leakage reduction from the perifoveal capillaries, or visual function was enhanced prior to eventual improvement in the fundus. Since HBO therapy has few contraindications nor side effects, it is an effective modality in the treatment of CME.