RESUMEN
DNA-based logic gates stimulate the development of molecular scale computers and show enormous potential in nanotechnology, biotechnology, and medicine. However, the reported detectors to date usually require one to label appropriate signal probes, resulting in not only a high cost but also potentially tedious manipulation. For the first time, we established a label-free logic gate by regarding the structure-related signal as output. Dual polarization interferometry (DPI) was employed to reveal the detailed conformational transitions occurring in the multiple-factor biomolecule interactions and then was utilized as a detection tool of logic gate. As a vital merit of this system, the dependence of the density output signal on the interaction with multiple-factor input can mimic the function of signal communication in OR, INHIBIT, and IDENTITY logic gates and the INHIBIT-OR cascade circuit. Additionally, the DPI signal with logic stringency can unambiguously distinguish conformational polymorphisms and compare structural stability. This study provides a new way for the construction of a label-free logic gate, supplements information deficiency of reaction details, and extends the application of DPI in logic operation.
Asunto(s)
G-Cuádruplex , Interferometría/métodos , Aptámeros de Nucleótidos/química , Dicroismo Circular , Computadores Moleculares , Cisteína/química , Colorantes Fluorescentes/química , Plomo/química , Mercurio/química , Conformación Molecular , Compuestos Orgánicos/química , Potasio/química , Espectrometría de FluorescenciaRESUMEN
In this paper, a rapid, high efficient, sensitive and inexpensive approach based on a combination of simple ultrasonic extract and capillary electrophoresis (CE) separation with electrochemical detection (ED), is described to identify herbs by comparing their CE-ED profiles (namely, CE-ED electropherograms). The proposed method takes advantage of ultra-small sample volume, low consumption of organic solvent, simple sample pretreatment and easy cleanup procedure. It was applied to analyze the CE-ED profiles of stems of herb Acanthopanax senticosus (Rupr. Et Maxim.) Harms from different sources and different parts (roots, rhizomes, stems and leaves) of this herb. By comparing peak number, peak height and peak height ratio, we found that the CE-ED profiles showed big differences for the herbs from the different sources and the different parts of this herb. In addition, the distribution of bioactive compounds (isofraxidin, rutin and chlorogenic acid) in the different parts of this herb and their content variations affected by the source were studied with the CE-ED method. Based on their own unique CE-ED profiles, these herbs from the different sources and the different parts of this herb could be easily distinguished. Therefore, the proposed approach could be used as a rapid, high efficient and sensitive method for the identification of herbal medicines.
Asunto(s)
Ácido Clorogénico/análisis , Cumarinas/análisis , Medicamentos Herbarios Chinos/química , Eleutherococcus/química , Rutina/análisis , Ácido Clorogénico/química , Cumarinas/química , Electroquímica , Electroforesis Capilar , Estructura Molecular , Oxidación-Reducción , Hojas de la Planta/química , Raíces de Plantas/química , Tallos de la Planta/química , Reproducibilidad de los Resultados , Rizoma/química , Rutina/químicaRESUMEN
A new oleanane triterpene, 16alpha-hydroxy-l3,28-epoxyoleanan-3-one 1, was isolated from the stem bark of Aegiceras corniculatum, together with protoprimulagenin, aegicerin, embelinone, syringic acid, gallic acid, isorhamnetin and isorhamnetin 3-O-alpha-L-rhamnofuranosyl-(1-->6)-beta-D-glucopyranoside. Their structures were determined by spectroscopic methods.
Asunto(s)
Ácido Oleanólico/análogos & derivados , Fitoterapia , Primulaceae , Humanos , Ácido Oleanólico/química , Corteza de la Planta , Triterpenos/químicaRESUMEN
Four new cholest-type steroidal glycosides, junceellosides A-D, isolated from the EtOH/CH(2)Cl(2) extracts of the South China Sea gorgonian coral Junceella juncea, were identified. Complete assignments of the (1)H and (13)C NMR chemical shifts for these compounds were achieved by means of one- and two-dimensional NMR techniques, including (1)H-(1)H COSY, HSQC, HMBC and NOESY spectra.
Asunto(s)
Antozoos/química , Diterpenos/química , Diterpenos/normas , Espectroscopía de Resonancia Magnética/métodos , Espectroscopía de Resonancia Magnética/normas , Valores de Referencia , Animales , Isótopos de Carbono , China , Diterpenos/análisis , Diterpenos/aislamiento & purificación , Glicósidos/análisis , Glicósidos/química , Glicósidos/aislamiento & purificación , Glicósidos/normas , Extractos Vegetales/análisis , Extractos Vegetales/química , Extractos Vegetales/aislamiento & purificación , Extractos Vegetales/normas , Protones , Esteroides/análisis , Esteroides/química , Esteroides/aislamiento & purificación , Esteroides/normasRESUMEN
The new triterpenoid taraxerol-3-beta-O-tridecyl ether was isolated from the aerial part of Derris triofoliata. The structure was established on the basis of spectral data.
Asunto(s)
Derris/química , Éteres , Triterpenos , Indicadores y Reactivos , Espectroscopía de Resonancia Magnética , Extractos Vegetales/análisis , Espectrometría de Masa por Ionización de ElectrosprayRESUMEN
Ten phenylethanoid glycosides, including two new ones, isolated from the aerial parts of the mangrove plant Acanthus ilicifolius were identified. The first complete assignments of the 1H and 13C NMR chemical shifts for these glycosides were achieved by means of 2D NMR techniques, including 1H-1H COSY, TOCSY, HSQC and HMBC spectra.
Asunto(s)
Acanthaceae/metabolismo , Glicósidos/química , Espectroscopía de Resonancia Magnética/métodos , Espectroscopía de Resonancia Magnética/normas , Modelos Moleculares , Fenoles/química , Isótopos de Carbono , China , Glicósidos/análisis , Conformación Molecular , Fenoles/análisis , Extractos Vegetales/análisis , Extractos Vegetales/química , Protones , Estándares de Referencia , EstereoisomerismoRESUMEN
From the aerial parts of Acanthus ilicifolius, a new aliphatic alcohol glycoside (ilicifolioside C) and two new (Z)-4-coumaric acid glycosides, (Z)-4-coumaric acid 4-O-beta-D-glucopyranoside and (Z)-4-coumaric acid 4-O-beta-D-apiofuranosyl-(1"-->2')-O-beta-D-glucopyranoside were isolated. The structural elucidations were based on the analyses of spectroscopic data. Z-Form 4-coumaric acid glycosides were first isolated from plant.