Photosensitizer-loaded gold nanocages for immunogenic phototherapy of aggressive melanoma.

Malignant melanoma remains the life-threatening form of skin cancer with high mortality and poor prognosis. Thus, an ideal melanoma therapeutic strategy is of immediate importance which can remove the primary tumor, as well as inhibit the metastasis and recurrence. Here, we report the fabrication of adjuvant monophosphoryl lipid A (MPLA) lipid bilayer-enveloped and photosensitizer indocyanine green (ICG)-loaded gold nanocages (MLI-AuNCs) for immunogenic phototherapy of aggressive melanoma. Hollow porous AuNCs are used as carriers to deliver MPLA and ICG, and protect ICG from photodegradation. Both AuNCs and ICG absorb near infrared (NIR) light and can be applied in controllable NIR-triggered photothermal and photodynamic combination therapy (PTT/PDT) of melanoma. MLI-AuNCs coated by thermosensitive lipid bilayer exhibit uniform size, good biocompatibility and bioavailability with prominent tumor accumulation, which further improve the PTT/PDT efficacy. MLI-AuNCs under NIR irradiation not only destroy the primary tumor by PTT/PDT, but also elicit robust antitumor immune response with melanoma associated antigens and MPLA released in situ. The released antigens and MPLA subsequently enhance the recruitment and maturation of dendritic cells, which further activate the effector T cells to inhibit metastases and recurrence of melanoma. This immunomodulatory-boosted PTT/PDT nanoplatform provides a new opportunity for highly aggressive melanoma treatment. STATEMENT OF SIGNIFICANCE: An ideal tumor therapeutic strategy not only can remove the primary tumor, but also inhibit metastasis and recurrence. Here, we introduced a versatile nanoplatform MLI-AuNCs for immunogenic phototherapy of aggressive melanoma. Adjuvant MPLA and photosensitizer ICG can be protected and co-delivered to the tumors by thermosensitive lipid-enveloped AuNCs. MLI-AuNCs exhibited prominent tumor accumulation ability and produced the potent PTT/PDT effect to destroy the primary tumors with a single dose of NIR irradiation, as well as elicited the strong antitumor immunity to inhibit the metastasis and relapse. This study may provide a potential therapeutic vaccination strategy against advanced melanoma and other difficult-to-treat cancers.

Polyethylenimine Hybrid Thin-Shell Hollow Mesoporous Silica Nanoparticles as Vaccine Self-Adjuvants for Cancer Immunotherapy.

Conventional adjuvants (e.g., aluminum) are insufficient to trigger cell-mediated immunity, which plays a crucial role in triggering specific immunity against cancer. Therefore, developing appropriate adjuvants for cancer vaccines is a central way to stimulate the antitumor immune response. Hollow mesoporous silica nanoparticles (HMSNs) have been proven to stimulate Th1 antitumor immunity in vivo and promote immunological memory in the formulation of novel cancer vaccines. Yet, immune response rates of existing HMSNs for anticancer immunity still remain low. Here, we demonstrate the generation of polyethylenimine (PEI)-incorporated thin-shell HMSNs (THMSNs) through a facile PEI etching strategy for cancer immunotherapy. Interestingly, incorporation of PEI and thin-shell hollow structures of THMSNs not only improved the antigen-loading efficacy and sustained drug release profiles but also enhanced the phagocytosis efficiency by dendritic cells (DCs), enabled DC maturation and Th1 immunity, and sustained immunological memory, resulting in the enhancement of the adjuvant effect of THMSNs. Moreover, THMSNs vaccines without significant side effects can significantly reduce the potentiality of tumor growth and metastasis in tumor challenge and rechallenge models, respectively. THMSNs are considered to be promising vehicles and excellent adjuvants for the formulation of cancer vaccines for immunotherapy.

Assessment of AgNPs exposure on physiological and biochemical changes and antioxidative defence system in wheat (Triticum aestivum L) under heat stress.

The present study was designed to check the role of silver nanoparticles (AgNPs) on physiological, biochemical parameters and antioxidants of wheat (Triticum aestivum L.) under heat stress. Plant extract of Moringa oleifera was used for AgNPs synthesis followed by characterization through UV-Vis spectroscopy, SEM, XRD and Zeta analyser. Heat stress was applied in range of 35-40°C for 3 hrs/ day for 3 days to wheat plants at trifoliate stage. Heat stress decreased the RWC (13.2%), MSI (16.3%), chl a (5.2%), chl b (4.1%) and TCCs (9.9%). Wheat plants treated with AgNPs showed significant increase in RWC (12.2%), MSI (26.5%), chl a (10%), chl b (16.4%), TCCs (19%), TPC (2.4%), TFC (2.5%), TASC (2.5%), SOD (1.3%), POX (1.5%), CAT (1.8%), APX (1.2%) and GPX (1.4%), under heat stress. Lower concentration of AgNPs (50 mg/l) decreased the sugar (5.8%) and proline contents (4%), while increase was observed in higher AgNPs concentrations. Overall, AgNPs treatment enhanced thermo-tolerance in wheat plants, but the mechanism of AgNPs action needs further investigation at genome and proteome level in wheat plants under heat stress.

Green synthesis and evaluation of silver nanoparticles for antimicrobial and biochemical profiling in Kinnow (Citrus reticulata L.) to enhance fruit quality and productivity under biotic stress.

Green synthesis of silver nanoparticles (AgNPs) by utilising plant extract is an emerging class of nanotechnology. It revolutionizes all the field of biological sciences by synthesizing chemical free AgNPs. In the present study, AgNPs were synthesised by utilising Moringa oleifera leaves as the main reducing and stabilising agent and characterised through UV-visible spectroscopy, zeta analyser, X-ray diffraction spectroscopy (XRD), energy dispersive X-ray (EDX), and scanning electron microscopy (SEM). The different concentrations of biosynthesised AgNPs (10, 20, 30, and 40 ppm) were exogenously applied on the already infected plants (canker) of Citrus reticulata at different day intervals. The AgNPs at a concentration of 30 ppm was found to be most suitable concentration for creating the resistance against canker disease in Citrus reticulata. The enzymatic activities were also explored and it was found that 30 ppm concentration of biosynthesised AgNPs significantly reduced the biotic stress. Fruit quality and productivity parameters were also assessed and it was found that fruit quality and productivity were significant in response to 30 ppm concentration of biosynthesised AgNPs. The present work highlights the potent role of biosynthesised AgNPs, which can be used as biological control of citrus diseases and ultimately improving the quality and productivity of Citrus.

Effect of silver nanoparticles and silver nitrate on growth of rice under biotic stress.

This study was organised to check the effect of silver nanoparticles and silver nitrate on rice growth against biotic stress. Silver nanoparticles were synthesised by using plant extract as reducing agent, followed by characterisation through UV Vis spectroscopy, XRD, EDS and SEM. Aspergillus application significantly reduced rice plant fresh mass (0.9%), dry mass (0.21%), root length (2.3%), shoot length (5.2%) and root number (1%) in comparison to control. Similarly, leaf area, leaf fresh mass, dry mass and leaf number were also reduced by 23.1, 0.02, 0.11 and 0.9%, respectively. AgNPs and AgNO3 treatments increased the root length (16.2 & 12.8%), shoot length (21 & 20%), root number (8.1 & 6.8%), plant fresh weight (6.4 & 5%) and plant dry weight (4.6 & 3.5%) in 75mg/l treatment of AgNPs and AgNO3 respectively. Similarly, AgNPs and AgNO3 treatment (75 mg/l concentrations) reflected remarkable increase in leaf area (58.8 & 57.2 %), leaf number (4.3 & 3.7 %), leaf fresh weight (1.7 & 1.4 %) and leaf dry weight (0.9 & 0.8 %). Overall AgNPs showed more significant results as compared to AgNO3. The quantity of aflatoxins ranged from 3.1 to 7.7 µg/kg against tolerable limit (4 µg/kg). Overall AgNPs and AgNO3 treatments showed significant results and it could be considered as a strategy for aflatoxin management in rice plants.

The effect of older-person centered and integrated health management model on multiple lifestyle behaviors: A randomized controlled trial from China.

OBJECTIVE: To investigate the effectiveness of the older-centered Integrated Health Management Model Project (OPCHMP) for multiple lifestyle behaviours in the elderly. METHODS: A 2-arm, parallel, randomized controlled trial was conducted in Nanjing. The elderly were recruited from multiple community health service centres. The intervention group was intervened and received a personalized, 2-year OPCHMP. The control group only received usual care. Adherence to healthy lifestyle behaviours (ATHLBS) is the primary outcome, obtained through a self-reported composite health behaviour score. The secondary outcomes were health indicators. General estimating equation models were performed to analyse longitudinal dichotomous data and continuous data. RESULTS: 637 (intervention = 323; control = 314) participants were included in the study. The participants mean age was 70.53 ±â€¯6.07 years. Significant ATHLBS correction was achieved after 24-month follow-up in the intervention group, comparing to controls. And the intervention group reported significantly better health indicators. CONCLUSION: OPCHMP had positive effect on multiple lifestyle habits in elderly population, which is very encouraging.

Green synthesis and characterisation of silver nanoparticles and their effects on antimicrobial efficacy and biochemical profiling in Citrus reticulata.

The synthesis of nanoparticles by utilising plant extract has revolutionised the field of nanotechnology. In the present study, AgNPs were synthesised by utilising the leaves of Moringa oleifera as reducing and stabilising agent. UV-visible spectroscopy showed characteristic surface plasmon band in the range of 413-420 nm. Scanning electron microscopy (SEM) elucidated rectangular segments fused together. X-ray diffraction (XRD) analysis confirmed the crystalline nature of AgNPs and presence of metallic silver ions was confirmed by energy dispersive X-ray (EDX). The different concentrations (10, 20, 30 and 40 ppm) of AgNPs were exogenously applied on Citrus reticulata to record the disease incidence at different day intervals. The disease intensity was progressively increased in all the applied treatments with the passage of time. The 30 ppm concentration of AgNPs was found to be most suitable concentration for creating the resistance against brown spot disease. Moreover, the effects of AgNPs were also assessed for biochemical profiling in C. reticulata. The enhanced production of endogenous enzymes and non-enzymatic components was observed in response to 30 ppm concentration of AgNPs. The present work highlighted that green synthesised AgNPs can be as used as biological control of citrus diseases and the enhanced production of secondary metabolites antioxidants.

In vitro seed germination and biochemical profiling of Artemisia absinthium exposed to various metallic nanoparticles.

In the recent years, nanotechnology has attained much attention in the every field of science. The synthesis, characterisation and applications of metallic nanoparticles (MNPs) have become an important branch of nanotechnology. In the current study, MNPs were synthesised through polyols process and applied in vitro to study their effect on medicinally important plant : Artemisia absinthium. The current study strives to check the effect of MNPs, i.e., Ag, Cu and Au on seed germination, root and shoot length, seedling vigour index (SVI) and biochemical profiling in A. absinthium. The seeds were inoculated on MS medium supplemented with various combinations of MNPs suspension. The seed germination was greatly influenced upon the application of MNPs and was recorded highest for the silver nanoparticles (AgNPs) suspensions. The best result for seed germination (98.6%) was obtained in MS medium supplemented with AgNPs as compared to control (92.9%) and other nanoparticles, i.e., copper (69.6%) and gold (56.5%), respectively, after 35 days of inoculation. Significant results were obtained for root length, shoot length and SVI in response to application of AgNPs as compared to copper nanoparticles (CuNPs) and gold nanoparticles (AuNPs). These nanoparticles (NPs) could induce stress in plants by deploying the endogenous mechanism. In response to these stresses, plants produce various defence compounds. Total phenolic content (TPC) and total flavonoid content (TFC) were significant in the MS medium supplemented with AgNPs as compared to other NPs, while DPPH radical scavenging assay (RSA) was highest in AuNPs treated plantlets. The MNPs showed higher toxicity level and enhanced secondary metabolites production, total phenolic content, total flavonoid content, antioxidant activity, superoxide dismutase (SOD) activity and total protein content.