OsbZIP1 regulates phosphorus uptake and nitrogen utilization, contributing to improved yield.

Increasing nutrient uptake and use efficiency in plants can contribute to improved crop yields and reduce the demand for fertilizers in crop production. In this study, we characterized a rice mutant, 88n which showed long roots under low nitrogen (N) or phosphorus (P) conditions. Low expression levels of N transporter genes were observed in 88n root, and total N concentration in 88n shoots were decreased, however, C concentrations and shoot dry weight in 88n were comparable to that in WT. Therefore, 88n showed high nitrogen utilization efficiency (NUtE). mRNA accumulation of Pi transporter genes was higher in 88n roots, and Pi concentration and uptake activity were higher in 88n than in WT. Therefore, 88n also showed high phosphorus uptake efficiency (PUpE). Molecular genetic analysis revealed that the causal gene of 88n phenotypes was OsbZIP1, a monocot-specific ortholog of the A. thaliana bZIP transcription factor HY5. Similar to the hy5 mutant, chlorophyll content in roots was decreased and root angle was shallower in 88n than in WT. Finally, we tested the yield of 88n in paddy fields over 3 years because 88n mutant plants showed higher PUpE and NUtE activity and different root architecture at the seedling stage. 88n showed large panicles and increased panicle weight/plant. Taken together, a mutation in OsbZIP1 could contribute to improved crop yields.

Contamination and ecological risk assessment of Cr, As, Cd and Pb in water and sediment of the southeastern Bay of Bengal coast in a developing country.

Safe levels of heavy metals in the surface water and sediment of the eastern Bay of Bengal coast have not been universally established. Current study characterized heavy metals such as arsenic (As), chromium (Cr), cadmium (Cd) and lead (Pb) in surface water and sediments of the most important fishing resource at the eastern Bay of Bengal coast, Bangladesh. Both water and sediment samples were analyzed using inductively coupled plasma mass spectrometer. Considering both of the seasons, the mean concentrations of Cr, As, Cd, and Pb in water samples were 33.25, 8.14, 0.48, and 21.14 µg/L, respectively and in sediment were 30.47, 4.48, 0.20, and 19.98 mg/kg, respectively. Heavy metals concentration in water samples surpassed the acceptable limits of usable water quality, indicating that water from this water resource is not safe for drinking, cooking, bathing, and any other uses. Enrichment factors also directed minor enrichment of heavy metals in sediment of the coast. Other indexes for ecological risk assessment such as pollution load index (PLI), contamination factor (CF), geoaccumulation index (Igeo), modified contamination degree (mCd), and potential ecological risk index (PERI) also indicated that sediment of the coastal watershed was low contamination. In-depth inventorying of heavy metals in both water and sediment of the study area are required to determine ecosystem health for holistic risk assessment and management.

Animal protein-soybean oil-based broiler diet optimizes net profit at the expense of desirable ω-6 fatty acids from the breast muscle of the broiler chicken.

Total 288 Ross-308-day-old male broiler chicks were randomly distributed into six dietary treatment groups in a two-way ANOVA with 2 × 3 factorial arrangements (two factors, i.e., dietary protein and energy having two types of protein, e.g., plant, animal and three different sources of energy, e.g., soybean oil, rice bran oil and sunflower oil) to justify if animal protein-soybean oil based broiler diet optimizes net profit at the expense of desirable ω-6 fatty acids in the breast muscle of the broiler chicken. Average daily feed intake (ADFI), final live weight (FLW), average daily gain (ADG), feed efficiency (FE), carcass characteristics, cardio-pulmonary morphometry, fatty acid profile of the breast muscle and cost-benefit analysis were measured. Results indicated that animal protein significantly increased 4.27% FLW, 6.13% ADFI, 4.31% ADG and 2.93% wing weight. Accordingly, soybean oil increased 4.76% FLW, 3.80% ADG and 1.36% dressing percentage at the expense of 12.07% proventriculus weight compared with sunflower oil. The generalized linear model identified no interaction effects of the sources of protein and energy on overall performance of the birds. Replacement of vegetable protein by animal protein decreased 14.01% ∑ω-3, 12.16% ∑ω-6 and 12.21% sum of polyunsaturated fatty acids (∑PUFA) and concomitantly increased 10.82% sum of saturated fatty acids (∑SFAs) in the breast muscle (Pectoralis major). Accordingly, replacement of sunflower oil by soybean oil decreased 29.17% ∑ω-3, 6.71% ∑ω-6, 11.62% sum of monounsaturated fatty acids (∑MUFAs) and 7.33% ∑PUFAs and concurrently increased 18.36% ∑SFAs in the breast muscle of the broiler birds. It was concluded that animal protein-soybean oil-based broiler diet optimized net profit at the expense of desirable ω-3 and ω-6 fatty acids in the breast muscle of the broiler chicken.

Diagnostic dilemma of celiac disease presenting with weight loss and secondary amenorrhea: A case report.

RATIONALE: Celiac disease (CD) is autoimmune enteropathy affecting the proximal small intestinal mucosa. It is caused by insensitivity to gluten, a protein predominantly presented in wheat. CD is classically associated with gastrointestinal symptoms. The non-classic clinical presentation of CD can present with other organ involvement. Non-human leukocyte antigens genes are associated with atypical forms. PATIENTS CONCERN: We reported a case of 30-year-old female who presented with progressive pallor, amenorrhea, and unexplained weight loss with generalized body weakness. Her body mass index was 20. The patient was having no other systemic manifestations. DIAGNOSIS: This paper reports a case of a female patient having CD without its typical features. Her laboratory evaluation revealed microcytic anemia. Anti-TTg IgA and Anti-TTG IgG antibodies were raised, ferritin and folate were low, and there was mild hyperbilirubinemia. However, follicle-stimulating hormone, luteinizing hormone, and serum estradiol levels were normal. She was diagnosed with a case of anemia resulting from malabsorption caused by CD. INTERVENTIONS: A management plan was devised based on a strict gluten-free diet. The patient received supplements containing folates, iron, calcium, zinc, and vitamins A, D, E, B6, and B12. OUTCOMES: After 3 months of treatment with strict gluten-free diet patient showed remarkable improvement. Her hemoglobin level raised with weight gain. Her normal menstrual cycle was restored with complete resolution of symptoms at 1 year follow-up. LESSONS: The pathogenesis of the atypical CD is multifactorial, but impaired uptake of micronutrients from the duodenum is the most likely cause, even if other common features of classical forms, such as bloating and diarrhea, are absent. Lack of awareness about atypical forms may lead to under-diagnoses of the disease. The physicians should consider the atypical presentations of CD to avoid the under-diagnoses of this multisystem disorder.

The impact of different levels of L-methionine (L-Met) on carcass yield traits, serum metabolites, tibial characters, and profitability of broilers fed conventional diet.

OBJECTIVES: The experiment was undertaken to investigate the performances of broilers with respect to meat yield traits, leg bone quality, blood metabolites, and economic profitability fed conventional diets supplemented with L-methionine (L-Met). MATERIALS AND METHODS: Day-old broiler chicks (n = 144) of either sex were used to conduct the experiment from d1 to 33 days in a battery cage rearing system. Birds were distributed randomly into four dietary treatments, i.e., D0 (DL-Met), D1 (0.20% L-Met), D2 (0.25% L-Met), and D3 (0.30% L-Met) in a completely randomized design. Broiler chicks were fed complete starter ration for the first 2 weeks and then test diets were supplied ad libitum from d15 to 33 days. All the formulated rations had the same calorie and proteinous values. Similar housing, feeding, and rearing management were provided to the birds for all the experimental period. Data on carcass yield traits, such as dressing %, thigh, breast, back, drumstick, shank, neck, and wing weights, etc, were measured on the last day of the trial. Blood serum profile (total protein, glucose, albumin, uric acid, creatinine, and triglycerides), right tibial bone traits (bone weight, bone width), and mineral concentrations (Ca% and P%) were also assessed on the last day of the experiment. The economic profitability of broilers fed on the L-Met diet was also measured in this study. RESULTS: The results revealed that except for dressing % (p < 0.05) and back weight (p < 0.01); all other meat characteristics measured this study were found similar (p > 0.05) between treatments. The highest dressing % and back weight were observed in the D3 group and the lowest being in D0. Blood serum metabolites did not differ (p > 0.05) among treatments. Leg bone traits of broilers were found similar (p > 0.05) between treatments. Higher profit (p < 0.01) and lower production cost (p < 0.05) were observed in the birds fed the D3 diet than other treatments. CONCLUSION: It can be inferred that broilers might show improved dressed yield and profitable broiler production fed on L-Met supplemented diet (D3).

Trace elements in two staple cereals (rice and wheat) and associated health risk implications in Bangladesh.

Concentrations of fourteen trace elements (Cd, As, Pb, Cr, Ni, Zn, Se, Cu, Mo, Mn, Sb, Ba, V and Ag) in the composite samples of most frequently consumed two staple foods, i.e. rice and wheat (collected from 30 different agroecological zones for the first time in Bangladesh) were measured by ICP-MS. The mean concentrations (mg/kg fresh weight) of Cd, As, Pb, Cr, Ni, Zn, Se, Cu, Mo, Mn, Sb, Ba, V and Ag were found as 0.088, 0.321, 0.713, 0.183, 0.213, 13.178, 0.0256, 1.985, 0.102, 4.654, 0.0033, 0.144, 0.081 and 0.007 and 0.011, 0.281, 0.221, 0.352, 0.145, 15.472, 0.245, 1.894, 0.209, 22.077, 0.0012, 3.712, 0.023 and 0.0013 in rice and wheat samples, respectively. Dietary risk of human health (non-carcinogenic and carcinogenic risks) was assessed by USEPA deterministic approaches. Total target hazard quotient (THQ) values for As and Pb were higher than 1, suggesting that people would experience significant health risks from consuming rice and wheat. However, the THQ of other metals were all less than 1. Also, the estimation showed that the target carcinogenic risk (TR) of As and Pb exceeded the accepted risk level of 1 × 10(-6). Moreover, concerning the nutritional requirements of essential elements for a sound health, the recommended doses for the daily intake of Mn was conveniently supplied by the studied cereals; however, Cr, Zn, Se, Cu and Mo were below the recommend daily allowances (RDAs). Thus, the carcinogenic and non-carcinogenic risk of As and Pb with lower supplementation of essential elements via staple foods for Bangladeshi people is a matter of concern.

System dynamics approach for modeling of sugar beet yield considering the effects of climatic variables.

BACKGROUND: The aim of this study was to develop a system dynamics model for computation of yields and to investigate the dependency of yields on some major climatic parameters, i.e. temperature and rainfall, for Beta vulgaris subsp. (sugar beet crops) under future climate change scenarios. RESULTS: A system dynamics model was developed which takes account of the effects of rainfall and temperature on sugar beet yields under limited irrigation conditions. A relationship was also developed between the seasonal evapotranspiration and seasonal growing degree days for sugar beet crops. The proposed model was set to run for the present time period of 1993-2012 and for the future period 2013-2040 for Lethbridge region (Alberta, Canada). The model provides sugar beet yields on a yearly basis which are comparable to the present field data. It was found that the future average yield will be increased at about 14% with respect to the present average yield. CONCLUSION: The proposed model can help to improve the understanding of soil water conditions and irrigation water requirements of an area under certain climatic conditions and can be used for future prediction of yields for any crops in any region (with the required information to be provided). The developed system dynamics model can be used as a supporting tool for decision making, for improvement of agricultural management practice of any region.

Spatial distribution and importance of potential perfluoroalkyl acid precursors in urban rivers and sewage treatment plant effluent--case study of Tama River, Japan.

Production and use of perfluorooctane sulfonate (PFOS) is regulated worldwide. However, numerous potential precursors that eventually decompose into PFOS and other perfluoroalkyl acids (PFAAs) such as perfluorooctanoic acid (PFOA) are still being used and have not been studied in detail. Therefore, knowledge about the levels and sources of the precursors is essential. We investigated the total concentration of potential PFAA precursors in the Tama River, which is one of the major rivers flowing into the Tokyo Bay, by converting all the perfluorinated carboxylic acid (PFCA) and perfluoroalkyl sulfonic acid (PFSA) precursors into PFCAs by chemical oxidation. The importance of controlling PFAA precursors was determined by calculating the ratios of PFCAs formed by oxidation to the PFAAs originally present (ΣΔ[PFCAC4-C12]/Σ[PFAAs]before oxidation) (average = 0.28 and 0.69 for main and tributary branch rivers, respectively). Higher total concentrations of Δ[PFCAs] were found in sewage treatment plant (STP) effluents. However, the ratios found in the effluents were lower (average = 0.21) than those found in the river water samples, which implies the decomposition of some precursors into PFAAs during the treatment process. On the other hand, higher ratios were observed in the upstream water samples and the existence of emission sources other than the STP effluents was indicated. This study showed that although the treatment process converting a part of the PFAA precursors into PFAAs, STPs were important sources of precursors to the Tama River. To reduce the levels of PFAAs in the aquatic environment, it is necessary to reduce the emission of the PFAA precursors as well.

Antioxidant, antibacterial and cytotoxic effects of the phytochemicals of whole Leucas aspera extract.

OBJECTIVE: To investigate the antioxidant, antibacterial and cytotoxic activity of whole Leucas aspera (Labiatae) (L. aspera) alcoholic extract. METHODS: Whole L. aspera powder was extracted by absolute ethanol (99.50%). The ethanolic extract was subjected to antioxidant, antibacterial and brine shrimp lethality assay. RESULTS: The extract showed potent radical scavenging effect (antioxidant) with IC50 value of (99.58±1.22) µg/mL which was significant (P<0.01) in comparison to ascorbic acid with IC50 value of (1.25±0.95) µg/mL. In case of antibacterial screening, the extract showed notable antibacterial effect against the tested microbial strains. Significant (P<0.05) zone of inhibitions against Gram positive Bacillus subtilis [(12.00±1.32) mm] and Bacillus megaterium [(13.00±1.50) mm], Staphylococcus aureus [(8.00±0.50) mm] and Gram negative Salmonella typhi [(6.00±0.50) mm], Salmonella paratyphi [(8.00±1.00) mm], Shigella dysenteriae [(9.00±1.32) mm] and Vibrio cholerae [(9.00±0.66) mm] was observed. In brine shrimp lethality bioassay, the extract showed the LC50 value as (181.68±2.15) µg/mL which was statistically significant (P<0.01) compared to positive control vincristine sulfate [LC50=(0.76±0.04) µg/mL]. CONCLUSIONS: The results demonstrate that the ethanolic extract of L. aspera could be used as antibacterial, pesticidal and various pharmacologic actives.