RESUMEN
Тhe bioenergetic processes in the heart mitochondria of Wistar rats with fructose-induced insulin resistance was investigated in female animals with different estrogen status. Respiration studies on isolated heart mitochondria by the polarographic method revealed that estrogen deficiency reduced complex IV activity, while its combination with high-fructose diet induced additional disturbances in the coupling of respiration and oxidative phosphorylation at the level of complex I of the electron transport chain. Exogenous 17b-estradiol inhibited the development of mitochondrial dysfunction in cardiomyocytes of ovariectomized rats with insulin resistance.
Asunto(s)
Respiración de la Célula/efectos de los fármacos , Estradiol/farmacología , Estrógenos/farmacología , Resistencia a la Insulina , Mitocondrias Cardíacas/metabolismo , Animales , Dieta , Complejo I de Transporte de Electrón/metabolismo , Complejo IV de Transporte de Electrones/metabolismo , Femenino , Fructosa/administración & dosificación , Fructosa/toxicidad , Mitocondrias Cardíacas/efectos de los fármacos , Miocitos Cardíacos/efectos de los fármacos , Miocitos Cardíacos/metabolismo , Ovariectomía , Fosforilación Oxidativa , Ratas , Ratas WistarRESUMEN
Using the method of luminescent microscopy, the catecholamine (CA) and serotonin (S) content was examined in spleen structures, and the interrelations between them were traced in rats before and 15 minutes, 1, 2 and 4 hours after the acupuncture in LI 4 and GV 14 points. It was found that after acupuncture, neurotransmitter content in splenic red and white pulp was changed already in 15 minutes. The changes of CA and S content in granular luminescent cells (GLC) of the germinal centers of spleen lymphoid nodules had a wavy character. At the same time, the correlations between monoamine content in the central arteriole wall and in germinal center GLCs, as well as that one between GLCs and germinal center lymphocytes, became strong positive. The data obtained suggest the presence of an immune-stimulating component in acupuncture, which became apparent during the first hour and persisted for 4 hours after a single acupuncture in GV 14 and LI 4 points.
Asunto(s)
Puntos de Acupuntura , Catecolaminas , Centro Germinal , Serotonina , Bazo , Animales , Catecolaminas/inmunología , Catecolaminas/metabolismo , Centro Germinal/citología , Centro Germinal/inmunología , Centro Germinal/metabolismo , Masculino , Ratas , Serotonina/inmunología , Serotonina/metabolismo , Bazo/citología , Bazo/inmunología , Bazo/metabolismo , Factores de TiempoRESUMEN
Bilateral one-hour ischemia of the kidneys in rats causes essential metabolic shifts in the renal tissues, and diminished intensity of the energetic status as a result of which the intactness of the cell membranes and the main functions of the nephron are disturbed. Pharmacological correction of postischemic disorders by the alpha-adrenergic blocking agent pyrroxane increases the activity of oxidative processes and intensifies the glycolysis rate. Improvement of the energy provision of the nephron function occurs in parallel with restoration of the diuresis level and processes of filtration and reabsorption.