Aspergillus oryzae fermented germinated soybean extract alleviates perimenopausal symptoms in ovariectomised rats.

BACKGROUND: Soybeans have been widely used to alleviate climacteric symptoms. In this study, we investigated the oestrogenic activities of isoflavones extracted from Aspergillus oryzae-challenged germinated soybeans (AO-GS). Eight-week-old virgin Sprague-Dawley female rats were ovariectomised (OVX). The rats were orally administered 0.1 mg kg(-1) 17α-ethinyl oestradiol or three different doses of AO-GS (0.5, 1.0 2.0 g kg(-1) day(-1)) in distilled water for 6 weeks, while control rats were administered vehicle alone. Uterine weights and levels of oestradiol and testosterone in serum were measured. In addition to serum parameters, bone parameters were also acquired by using micro-computed tomography scanning. RESULTS: Treatments of OVX rats with AO-GS changed the secretory profile of serum oestradiol and testosterone. Serum oestradiol levels were significantly increased in OVX rats treated with and AO-GS (0.5, 1.0, 2.0 g kg(-1) day(-1)), while serum testosterone levels were not significantly increased in OVX rats treated with 1.0 g kg(-1) day(-1) of AO-GS. Furthermore, AO-GS (2.0 g kg(-1) day(-1)) significantly attenuated bone loss, increased trabecular bone volume fraction and trabecular thickness, and significantly decreased trabecular pattern factor. CONCLUSION: AO-GS treatments caused moderate oestrogenic activity in OVX rats compared to those treated with oestradiol, suggesting the potential for the use of AO-GS in the treatment of menopausal symptoms and in osteoporosis caused by oestrogen deficiency.

Photoprotective effects of galacto-oligosaccharide and/or Bifidobacterium longum supplementation against skin damage induced by ultraviolet irradiation in hairless mice.

This study aimed at examining whether oral administration of galacto-oligosaccharide (GOS) and Bifidobacterium longum, individually or in combination, could exert photoprotective effects on the skin of hairless mice. GOS and/or Bifidobacterium were administered orally to hairless mice for 12 weeks. Mice were irradiated with UV light daily for four consecutive days. GOS administration increased the water-holding capacity of the skin and prevented transepidermal water loss compared with the control. A reduction in the erythema formation of 16.8% was also observed in the GOS-treated group compared with the control, and CD44 gene expression was significantly increased. Oral administration of GOS or Bifidobacterium significantly increased TIMP-1 and Col1 mRNA expression compared with the control. Our findings support that prebiotics, including GOS, are beneficial not only to the intestine, but also to the skin, and present the possibility of new nutritional strategies for the prevention of UV-induced skin damage.