RESUMEN
The anti-inflammation effect of aqueous Phyllanthus emblica L. extract (APE) and its possible underlying mechanism in dextran sulfate sodium (DSS)-induced mice chronic colonic inflammation were studied. APE treatment significantly improved the colitic symptoms, including ameliorating the shortening of the colon, increasing the DSS-induced body weight loss, reducing the disease activity index, and reversing the condition of colon tissue damage of mucus lost and goblet cell reduction. Overproduction of serum pro-inflammatory cytokines were suppressed by the treatment of APE. Gut microbiome analysis showed that APE remodeled the structure of gut bacteria in phylum and genus levels, upregulating the abundance of phylum Bacteroidetes, family Muribaculaceae, and genus Bacteroides and downregulating the abundance of phylum Firmicutes. The reshaped gut microbiome caused metabolic functions and pathway change with enhanced queuosine biosynthesis and reduced polyamine synthesis pathway. Colon tissue transcriptome analysis further elucidated APE-inhibited mitogen-activated protein kinase (MAPK), cytokine-cytokine receptor interaction, and tumor necrosis factor (TNF) signaling pathways and the expressions of the genes that promote the progress of colorectal cancer. It turned out that APE reshaped the gut microbiome and inhibited MAPK, cytokine-cytokine receptor interaction, and TNF signaling pathways as well as the colorectal-cancer-related genes to exert its colitis protective effect.
Asunto(s)
Colitis , Microbioma Gastrointestinal , Hominidae , Phyllanthus emblica , Animales , Ratones , Dextranos , Colitis/inducido químicamente , Colitis/tratamiento farmacológico , Colitis/genética , Inflamación , Citocinas/genética , Proteínas Quinasas Activadas por Mitógenos , Receptores de Citocinas , Expresión Génica , Sulfatos , Extractos Vegetales , SodioRESUMEN
In this study, a microalgal-bacterial consortium (MBC) was established in liquid digestate (LD) by optimizing sequencing batch reactor (SBR) operating parameters and microalgae inoculation to address the abovementioned challenges. The bacteria from LD SBR-Activated Sludge System effluent under the optimum conditions of 25⯰C, 7.0â¯g/L MLSS, 5â¯mg/L DO concentration, and 6â¯h hydraulic retention time with 0.5â¯mg/L DW Chlorella sp. BWY-1 could form stable MBCs outdoors in an airlift photoreactor. The stable MBC facilitates the continuous removal of nitrogen and phosphorus, promotes the accumulation of biomass and lipids, and contributes to the improvement of the sedimentation. The results from this study provided a new technique for the purification and utilization of LD, more importantly decreasing the environmental threat caused by improperly processed LD.