RESUMEN
Acupuncture is an emerging alternative therapy that has been beneficial for the pain of osteoarthritis (OA). However, the underlying mechanism of protective effect remains unclear. MCP1/CCR2 axis can be stimulated in various periods of OA, and we hypothesize that acupuncture may treat OA by regulating the MCP1/CCR2 axis. This study aimed to explore the effect of acupuncture at points ST35 and ST36 on the effects of hyperalgesia and cartilage in OA rats including the expression of chemokines, nerve growth factor (NGF), and inflammatory-related proteins. OA was induced in male Sprague-Dawley rats by anterior cruciate ligament transection at the right knee. The first acupuncture intervention was performed on the seventh day after surgery and once a day for seven weeks. The knee-pain-related behaviors, histology, and related protein were examined in this study. We have found that electroacupuncture at ST35 and ST36 can significantly alleviate the hyperalgesia and cartilage degeneration as well as reducing nerve sprouting in OA knee joint. Moreover, acupuncture treatment may inhibit the MCP1/CCR2 axis as well as down-regulate inflaming factor and NGF in cartilage and synovial tissue. The data presented here indicate that acupuncture exerts a protective effect against hyperalgesia and cartilage degeneration, and the mechanism might involve in chemokines and NGF pathway.
Asunto(s)
Terapia por Acupuntura , Quimiocina CCL2/metabolismo , Osteoartritis/complicaciones , Dolor/metabolismo , Dolor/prevención & control , Receptores CCR2/metabolismo , Transducción de Señal , Animales , Benzoxazinas/farmacología , Benzoxazinas/uso terapéutico , Cartílago Articular/patología , Citocinas/metabolismo , Ganglios Espinales/patología , Hiperalgesia/prevención & control , Mediadores de Inflamación/metabolismo , Articulación de la Rodilla/patología , Masculino , Factor de Crecimiento Nervioso/metabolismo , Neuronas/patología , Osteoartritis/tratamiento farmacológico , Ratas Sprague-Dawley , Receptor trkA/metabolismo , Compuestos de Espiro/farmacología , Compuestos de Espiro/uso terapéutico , Membrana Sinovial/patologíaRESUMEN
Objective To analyze the clinical treatment effect of traditional Chinese medicine and western medicine in the treatment of chronic obstructive pulmonary disease.Methods 160 patients with COPD from December 2014 to December 2016 in our hospital were enrolled in this study.and randomly divided into two groups.The control group was treated with western medicine only.The observation group was treated with traditional Chinese medicine and western medicine.The therapeutic effects of the two groups were compared.Results The effective rate was 88.75% in the observation group and 76.25% in the control group(P<0.05).After treatment, FEV1 was(1.85±0.42) L and FVC was(1.64±0.33) L in the observation group.In the control group, FEV1 was(2.56±0.35) L, FVC was(2.32±0.37) L, the improvement of the observation group was more obvious(P<0.05).The incidence of adverse reactions was 8.75% in the observation group and 7.50% in the control group, the difference was not statistically significant.Conclusion The clinical effect of traditional Chinese and western medicine in the treatment of COPD is remarkable and worthy of promotion.