RESUMEN
Hedyotis hedyotidea has been traditionally used for the treatment of arthritis, cold, cough, gastro-enteritis, headstroke, etc. But few studies have screened the active compounds from extracts of H. hedyotidea. In this study, the structure of the chemical constituents from stems of H. hedyotidea were determined and the immunosuppressive activity of the compounds was evaluated. The compounds were separated and purified with silica gel, gel column chromatographies and preparative HPLC, and their structures were identified by spectral methods such as MS and NMR. Eleven compounds were obtained and identified as(6S,9S) -vomifoliol (1), betulonic acid (2), betulinic acid (3), betulin(4), 3-epi-betulinic acid (5), ursolic acid (6), β-sitosterol (7), stigmast-4-en-3-one (8), 7β-hydroxysitosterol (9), (3β,7β) -7-methoxystigmast-5-en-3-ol (10) and morindacin (11). This is the first report of compounds 1, 2, 4, 8, 9, 10 and 11 from H. hedyotidea. Compounds 1, 2 and 8-11 were firstly isolated from the genus Hedyotis, and compounds 9 and 10 were isolated from the family Rubiaceae for the first time. The immunosuppressive activity of these compounds was tested using the lymphocyte transsormationtest. Compounds 4, 6 and 9 showed significant immunosuppressive activity.
Asunto(s)
Animales , Masculino , Ratones , Medicamentos Herbarios Chinos , Química , Farmacología , Hedyotis , Química , Inmunosupresores , Química , Farmacología , Linfocitos , Alergia e Inmunología , Espectrometría de Masas , Ratones Endogámicos C57BL , Estructura Molecular , Tallos de la Planta , QuímicaRESUMEN
<p><b>OBJECTIVE</b>To study the chemical constituents from Schisandra glaucescens.</p><p><b>METHOD</b>The chemical constituents were separated and purifed with silica gel, gel column chromatography preparative HPLC, and their structures were identified by such spectral methods as MS and NMR.</p><p><b>RESULT</b>Twelve compounds were separated from petroleum ether fractions, and identified as t-cadinol (1), alpha-cadinol (2), torreyol (3), (+)-ent-epicubenol (4), ent-T-muurolol (5), (-)-15-hydroxycalamenene (6), (-)-cubebol (7), 4-epi-cubebol (8), caryophyllenol-I (9), caryophyllenol-II (10), oxyphyllenodiols A (11), caryolane-1,9/3-diol (12).</p><p><b>CONCLUSION</b>Compounds 4, 6-12 were separated from the genus for the first time, while compounds 1-12 were separated from this plant for the first time.</p>
Asunto(s)
Cromatografía Líquida de Alta Presión , Medicamentos Herbarios Chinos , Química , Espectroscopía de Resonancia Magnética , Estructura Molecular , Tallos de la Planta , Química , Schisandra , Química , Sesquiterpenos , QuímicaRESUMEN
<p><b>OBJECTIVE</b>To study the active constituents from Alternanthera philoxeroides.</p><p><b>METHOD</b>The constituents were isolated with silica gel and Toyopearl HW-40C gel column chromatography and purified by HPLC. Their structures were elucidated by spectroscopy.</p><p><b>RESULT</b>Nine compounds were isolated and identified as phaeophytin a (1), pheophytin a' (2), oleanoic acid (3), beta-sitosterol (4), 3beta-hydroxystigmast-5-en-7-one (5), alpha-spinasterol (6), 24-methylenecycloartanol (7), cycloeucalenol (8), phytol (9).</p><p><b>CONCLUSION</b>Compounds 1,2,5,7-9 were isolated from this plant for the first time.</p>