RESUMEN
The authors regret for the changes in Fig. 6 as follow: [Figure presented] Fig. 6. HPLC-ELSD chromatograms of ten Anoectochilus, four Goodyera and one Ludisia species on AQ-C
RESUMEN
<p><b>OBJECTIVE</b>To provide DNA molecular marker for identification of Nelumbo nucifera by exploring the differences of nrDNA-ITS sequence of N. nucifera originated from different habitats.</p><p><b>METHOD</b>To compare nrDNA-ITS base sequence using specific PCR-ITS.</p><p><b>RESULT</b>The completed sequence of ITS and 5.8 S rDNA, and the partial sequences of 18S rDNA and 26S rDNA, totally 750 bp, from N. nucifera were obtained. The differences among N. nucifera from different habitats and from different cultivars were found.</p><p><b>CONCLUSION</b>The method can be used to identify N. nucifera among different species and to distinguish their fakes. It provided the basis for identifying N. nucifera from different geographical regions by comparison of their ITS sequences.</p>
Asunto(s)
Secuencia de Bases , China , ADN de Plantas , Química , Genética , Metabolismo , ADN Espaciador Ribosómico , Clasificación , Genética , Desoxirribonucleasa EcoRI , Metabolismo , Desoxirribonucleasas de Localización Especificada Tipo II , Metabolismo , Contaminación de Medicamentos , Geografía , Nelumbo , Clasificación , Genética , Filogenia , Plantas Medicinales , Clasificación , Genética , Reacción en Cadena de la Polimerasa , ARN Ribosómico , Genética , ARN Ribosómico 18S , Genética , Genética , Análisis de Secuencia de ADN , Especificidad de la EspecieRESUMEN
<p><b>OBJECTIVE</b>To promote its comprehensive utilization, the involatile constituents of Mentha haplocalyx were studied systematically.</p><p><b>METHOD</b>The chemical components were isolated and purified by silca gel column chromatography and recrystallization. The chemical structures were elucidated on the basis of physico-chemical properties and spectral data.</p><p><b>RESULT</b>Eight compounds were isolated and identified as: acacetin (I), tilianine (II), linarin (III), n-butyl-beta-D-fructopyranoside (IV), ursolic acid (V), oleanolic acid (VI), beta-sitosterol (VII), daucosterol (VIII).</p><p><b>CONCLUSION</b>Compounds I approximately V were obtained from this plant for the first time.</p>