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Métodos Terapéuticos y Terapias MTCI
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Sci Rep ; 10(1): 14165, 2020 08 25.
Artículo en Inglés | MEDLINE | ID: mdl-32843675

RESUMEN

In Malaysia, Piper sarmentosum or 'kaduk' is commonly used in traditional medicines. However, its biological effects including in vivo embryonic toxicity and tissue regenerative properties are relatively unknown. The purpose of this study was to determine zebrafish (Danio rerio) embryo toxicities and caudal fin tissue regeneration in the presence of P. sarmentosum aqueous extracts. The phytochemical components and antioxidant activity of the extract were studied using GC-MS analysis and DPPH assay, respectively. Embryo toxicity tests involving survival, heartbeat, and morphological analyses were conducted to determine P. sarmentosum extract toxicity (0-60 µg/mL); concentrations of 0-400 µg/mL of the extract were used to study tissue regeneration in the zebrafish caudal fin. The extract contained several phytochemicals with antioxidant activity and exhibited DPPH scavenging activity (IC50 = 50.56 mg/mL). Embryo toxicity assays showed that a concentration of 60 µg/mL showed the highest rates of lethality regardless of exposure time. Slower embryogenesis was observed at 40 µg/mL, with non-viable embryos first detected at 50 µg/mL. Extracts showed significant differences (p < 0.01) for tissue regeneration at all concentrations when compared to non-treated samples. In conclusion, Piper sarmentosum extracts accelerated tissue regeneration, and extract concentrations at 60 µg/mL showed the highest toxicity levels for embryo viability.


Asunto(s)
Antioxidantes/farmacología , Desarrollo Embrionario/efectos de los fármacos , Fitoquímicos/farmacología , Piper/química , Extractos Vegetales/farmacología , Plantas Medicinales/química , Regeneración/efectos de los fármacos , Pez Cebra/embriología , Aletas de Animales/efectos de los fármacos , Aletas de Animales/lesiones , Aletas de Animales/fisiología , Animales , Antioxidantes/aislamiento & purificación , Antioxidantes/toxicidad , Embrión no Mamífero/efectos de los fármacos , Femenino , Depuradores de Radicales Libres/aislamiento & purificación , Depuradores de Radicales Libres/farmacología , Depuradores de Radicales Libres/toxicidad , Cromatografía de Gases y Espectrometría de Masas , Corazón/efectos de los fármacos , Corazón/embriología , Masculino , Fitoquímicos/aislamiento & purificación , Fitoquímicos/toxicidad , Extractos Vegetales/aislamiento & purificación , Extractos Vegetales/toxicidad , Agua
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