[Study on decoction's effect of different processed rhizomes of Cibotium barometz on retinoic acid induced male rats osteoporosis].

This study compared the decoction's HPLC figures of the different processed rhizomes of Cibotium barometz including the raw, the sand-baked, the wined, the steamed and the salted, on the basis of which, with the sand-baked Drynaria fortunei decoction as the positive control group, comparingall groups' decoction, concentration of which was 104.2 g x L(-1), for 4 weeks, by their effects (s-TRAP and total scores of OPG, Ca, P, IL-6, TNF-alpha and IL-1) on retinoic acid induced male rats osteoporosis. The experiment results showed the sand-baked and the wined were better than the steamed, the salted and the raw;in the processing methods' selection, the sand-baked was a better heating method than the steamed and the rice wine was the better excipient than the salt. It provided a reference to explain the processing principle of rhizomes of C. barometz and work mechanism of anti-osteoporosis.

Effect of the RW-Cb and its active ingredient like P-acid and P-aldehyde on primary rat osteoblasts.

ETHNOPHARMACOLOGICAL RELEVANCE: RW-Cb, the first processed product of Cibotium barometz (L.) J. Sm. (Dicksoniaceae) in TCM has been widely used to treat osteoporosis, a major worldwide health problem, influencing more and more people in the word et al. To do research on RW-Cb, one processed product of Cibotium barometz (L.) J. Sm. (Dicksoniaceae) in TCM has been widely used to treat osteoporosis, a major worldwide health problem, influencing more and more people in the word et al. MATERIALS AND METHODS: The raw rhizome slices of Cibotium barometz were identified and both P-acid and P-aldehyde had a high purity. Primary rat osteoblasts were cultured and identified to be used for the assay. RESULTS: RW-Cb contributed the most to osteoblasts proliferation and the AKP activity among all the groups; osteoblasts proliferation effect of P-acid and P-aldehyde: the mixed group(high>middle>low)>the P-acid group (high>middle>low) or the P-aldehyde group(high>middle>low), P<0.001; AKP activity: the P-acid's high group was higher than P-aldehyde's high, the mixed of low and middle groups had no significant difference, the rest of groups' results were similar to the osteoblasts' proliferation, P<0.05. CONCLUSIONS: RW-Cb and its index constituents like P-acid and P-aldehyde could promote proliferation and differentiation of primary rat osteoblasts cultured in vitro, and it could be the theoretical basis for the treatment of osteoporosis.

[Detection on effect of different processed Cibotium barometz on osteoblasts by CCK-8].

The pancreatic enzyme-II type collagenase digestion method was adopted for primary culture of osteoblasts, inoculation and passage. They were identified by alkaline phosphatase dye-liquor. N-butanol extract fractions from different processed products of Cibotium barometz were prepared. The above osteoblasts were jointly cultured with protocatechuic acid, protocatechuic aldehyde, kojic acid and the mixed control liquid of the above three substances, and their proliferation was detected by CCK-8. Various n-butanol extract fractions from different processed products of C. barometz showed a significant proliferative effect on osteoblasts in the order of the wined > the heated > the salted > the sand-heated and wined system > the alcohol-processed > the steamed > the crude. The q test showed no significant difference among sand-heated, alcohol-processed and steamed C. barometz, no significant difference between heated and salted C. barometz. Various control substances also showed a certain proliferative effect on osteoblasts in the order of the mixed control > protocatechuic aldehyde > protocatechuic acid > kojic acid. The q test showed no significant difference between protocatechuic aldehyde and protocatechuic acid. All of n-butanol extract fractions from different processed products of C. barometz showed a significant effect on osteoblast proliferation, of which wined C. barometz showed the best effect. All of phenolic compounds such as protocatechuic aldehyde, protocatechuic acid and kojic acid showed a significant proliferative effect on osteoblasts.