RESUMEN
El objetivo del trabajo fue evaluar el efecto del aceite esencial Chrysactinia mexicana A. Gray en aislados clínicos de C. glabrata. Se obtuvo aceite esencial por arrastre de vapor de las flores, hojas y ramas secundarias de C. mexicana. Se realizó cromatografía de gases y espectrometría de masas para conocer la composición del aceite. Se hicieron ensayos de microdilución en fase logarítmica y fase estacionaria con Saccharomyces cerevisiae y aislados clínicos de C. glabrata para determinar la Concentración Mínima Inhibitoria (CMI) en presencia del aceite esencial. Los principales compuestos del aceite esencial fueron la piperitona (29.57 %), eucaliptol (26.86 %), a-terpineol (14.65 %), delta-3-careno (12.37 %) y linalool (3.56 %). Se determinaron las CMI del aceite esencial, para las cinco cepas utilizadas en las dos fases de crecimiento, siendo menor la CMI para S. cerevisiae que para los diferentes aislados clínicos de C. glabrata. El aceite esencial de C. mexicana tiene efecto fungicida en S. cerevisiae y los aislados clínicos de C. glabrata.
Asunto(s)
Humanos , Asteraceae , Candida glabrata , Cromatografía de Gases y Espectrometría de Masas , Medicina TradicionalRESUMEN
BACKGROUND: Inulin and other fructans are synthesized and stored in mezcal agave (Agave salmiana). Fructans provide several health benefits and have excellent technological properties, but only few data report their physiological effect when added in the diet. RESULTS: Here, we studied the physiological effects of fructans obtained from A. salmiana when added in the diet of Wistar rats. Results showed favorable changes on Wistar rats when the fructans was added to their diet, including the decrease of the pH in the feces and the increase of the number of lactic acid bacteria (CFU g-1 ) (Lactobacillus spp. and Bifidobacterium spp.), even these changes were enhanced with the synbiotic diet (fructans plus B. animalis subsp. lactis). Synbiotic diet, developed changes in the reduction of cholesterol and triglycerides concentrations in serum, with statistical differences (P < 0.05). Histological analysis of colon sections showed that synbiotic diet promoted colon cells growth suggesting that fructans from A. salmiana confer beneficial health effects through gut microbiota modulation. CONCLUSION: Our data underline the advantage of targeting the gut microbiota by colonic nutrients like specific structure of fructans from A. salmiana, with their beneficial effects. More studies are necessary to define the role of fructans to develop more solid therapeutic solutions in humans. © 2016 Society of Chemical Industry.
Asunto(s)
Agave/química , Disbiosis/prevención & control , Fructanos/uso terapéutico , Frutas/química , Microbioma Gastrointestinal , Extractos Vegetales/uso terapéutico , Prebióticos , Agave/crecimiento & desarrollo , Animales , Bifidobacterium/crecimiento & desarrollo , Bifidobacterium/aislamiento & purificación , Bifidobacterium animalis/crecimiento & desarrollo , Colon/citología , Colon/microbiología , Colon/patología , Disbiosis/sangre , Disbiosis/microbiología , Disbiosis/patología , Heces/química , Heces/microbiología , Liofilización , Fructanos/aislamiento & purificación , Frutas/crecimiento & desarrollo , Concentración de Iones de Hidrógeno , Hiperlipidemias/sangre , Hiperlipidemias/microbiología , Hiperlipidemias/patología , Hiperlipidemias/prevención & control , Mucosa Intestinal/citología , Mucosa Intestinal/microbiología , Mucosa Intestinal/patología , Lactobacillus/crecimiento & desarrollo , Lactobacillus/aislamiento & purificación , Masculino , México , Extractos Vegetales/aislamiento & purificación , Distribución Aleatoria , Ratas Wistar , SimbióticosRESUMEN
Agave salmiana is a fructan rich species that is widely distributed in Mexico. The aim of this investigation was to extract the fructans of A. salmiana and evaluate their prebiotic effect in 48 hours in vitro cultures of Bifidobacterium lactis and Lactobacillus acidophilus and to compare this effect with other available fructan sources. A significant difference in pH, optical density and biomass was found in the cultures depending on the source of fructans and the type of bacteria. It was possible to determine a dose-response effect of the A. salmiana fructans and the growth of the studied strains.
Asunto(s)
Agave/metabolismo , Bifidobacterium/metabolismo , Lactobacillus acidophilus/metabolismo , Prebióticos/microbiología , Agave/química , Agave/microbiología , Bifidobacterium/crecimiento & desarrollo , Fermentación , Fructanos/análisis , Fructanos/metabolismo , Lactobacillus acidophilus/crecimiento & desarrollo , México , Prebióticos/análisisRESUMEN
Colon cancer is a world concerning disease; it shows a high mortality rate and may be related to eating habits. Studies using inulin-like fructans, which are produced as energy supplies by several plants, have demonstrated a chemo-protective effect of these fructans in colon cancer. However, agavins a structurally different type of fructans from the Agave genus with demonstrated prebiotic effects, have been poorly studied for their possible protective effects in cancer. The aim of the present study was to evaluate the effect ofAgave fructan-rich diets in colon cancer progress using a rat model and "Agave mezcalero potosino" A. salmiana Otto ex Salm Dick, which is widely distributed in Mexico. Results showed a significant decrease (p<0.05) in early lesions of colon cancer (aberrant crypt foci) compared with the control group. These data suggest that fructans from A. salmiana may contribute to a reduction in the risk of colon cancer as well as inulin-like compounds.