RESUMEN
Based on the potential role of Na-K-Cl cotransporters (NKCCs) in epileptic seizures, the loop diuretic bumetanide, which blocks the NKCC1 isoforms NKCC1 and NKCC2, has been tested as an adjunct with phenobarbital to suppress seizures. However, because of its physicochemical properties, bumetanide only poorly penetrates through the blood-brain barrier. Thus, concentrations needed to inhibit NKCC1 in hippocampal and neocortical neurons are not reached when using doses (0.1-0.5â¯mg/kg) in the range of those approved for use as a diuretic in humans. This prompted us to search for a bumetanide derivative that more easily penetrates into the brain. Here we show that bumepamine, a lipophilic benzylamine derivative of bumetanide, exhibits much higher brain penetration than bumetanide and is more potent than the parent drug to potentiate phenobarbital's anticonvulsant effect in two rodent models of chronic difficult-to-treat epilepsy, amygdala kindling in rats and the pilocarpine model in mice. However, bumepamine suppressed NKCC1-dependent giant depolarizing potentials (GDPs) in neonatal rat hippocampal slices much less effectively than bumetanide and did not inhibit GABA-induced Ca2+ transients in the slices, indicating that bumepamine does not inhibit NKCC1. This was substantiated by an oocyte assay, in which bumepamine did not block NKCC1a and NKCC1b after either extra- or intracellular application, whereas bumetanide potently blocked both variants of NKCC1. Experiments with equilibrium dialysis showed high unspecific tissue binding of bumetanide in the brain, which, in addition to its poor brain penetration, further reduces functionally relevant brain concentrations of this drug. These data show that CNS effects of bumetanide previously thought to be mediated by NKCC1 inhibition can also be achieved by a close derivative that does not share this mechanism. Bumepamine has several advantages over bumetanide for CNS targeting, including lower diuretic potency, much higher brain permeability, and higher efficacy to potentiate the anti-seizure effect of phenobarbital.
Asunto(s)
Anticonvulsivantes/farmacología , Bencilaminas/farmacología , Bumetanida/farmacología , Fenobarbital/farmacología , Animales , Anticonvulsivantes/síntesis química , Anticonvulsivantes/química , Anticonvulsivantes/farmacocinética , Bencilaminas/síntesis química , Bencilaminas/química , Bencilaminas/farmacocinética , Encéfalo/efectos de los fármacos , Encéfalo/metabolismo , Bumetanida/análogos & derivados , Bumetanida/química , Bumetanida/farmacocinética , Evaluación Preclínica de Medicamentos , Sinergismo Farmacológico , Epilepsia/tratamiento farmacológico , Epilepsia/metabolismo , Femenino , Ratones , Oocitos , Fenobarbital/farmacocinética , Ratas Wistar , Convulsiones/tratamiento farmacológico , Convulsiones/metabolismo , Inhibidores del Simportador de Cloruro Sódico y Cloruro Potásico/química , Inhibidores del Simportador de Cloruro Sódico y Cloruro Potásico/farmacocinética , Inhibidores del Simportador de Cloruro Sódico y Cloruro Potásico/farmacología , Miembro 2 de la Familia de Transportadores de Soluto 12/metabolismo , Técnicas de Cultivo de Tejidos , Xenopus laevisRESUMEN
Febrile seizures are the most common type of convulsive events in children. It is generally assumed that the generalization of these seizures is a result of brainstem invasion by the initial limbic seizure activity. Using precollicular transection in 13-day-old rats to isolate the forebrain from the brainstem, we demonstrate that the forebrain is not required for generation of tonic-clonic convulsions induced by hyperthermia or kainate. Compared with sham-operated littermate controls, latency to onset of convulsions in both models was significantly shorter in pups that had undergone precollicular transection, indicating suppression of the brainstem seizure network by the forebrain in the intact animal. We have shown previously that febrile seizures are precipitated by hyperthermia-induced respiratory alkalosis. Here, we show that triggering of hyperthermia-induced hyperventilation and consequent convulsions in transected animals are blocked by diazepam. The present data suggest that the role of endogenous brainstem activity in triggering tonic-clonic seizures should be re-evaluated in standard experimental models of limbic seizures. Our work sheds new light on the mechanisms that generate febrile seizures in children and, therefore, on how they might be treated.
Asunto(s)
Hipertermia Inducida/efectos adversos , Prosencéfalo/fisiología , Convulsiones/etiología , Animales , Animales Recién Nacidos , Anticonvulsivantes/uso terapéutico , Temperatura Corporal/fisiología , Desnervación , Diazepam/uso terapéutico , Modelos Animales de Enfermedad , Agonistas de Aminoácidos Excitadores/toxicidad , Ácido Kaínico/toxicidad , Masculino , Ratas , Ratas Wistar , Convulsiones/tratamiento farmacológico , Factores de TiempoRESUMEN
OBJECTIVE: There is considerable interest in using bumetanide, a chloride importer Na-K-Cl cotransporter antagonist, for treatment of neurological diseases, such as epilepsy or ischemic and traumatic brain injury, that may involve deranged cellular chloride homeostasis. However, bumetanide is heavily bound to plasma proteins (~98%) and highly ionized at physiological pH, so that it only poorly penetrates into the brain, and chronic treatment with bumetanide is compromised by its potent diuretic effect. METHODS: To overcome these problems, we designed lipophilic and uncharged prodrugs of bumetanide that should penetrate the blood-brain barrier more easily than the parent drug and are converted into bumetanide in the brain. The feasibility of this strategy was evaluated in mice and rats. RESULTS: Analysis of bumetanide levels in plasma and brain showed that administration of 2 ester prodrugs of bumetanide, the pivaloyloxymethyl (BUM1) and N,N-dimethylaminoethylester (BUM5), resulted in significantly higher brain levels of bumetanide than administration of the parent drug. BUM5, but not BUM1, was less diuretic than bumetanide, so that BUM5 was further evaluated in chronic models of epilepsy in mice and rats. In the pilocarpine model in mice, BUM5, but not bumetanide, counteracted the alteration in seizure threshold during the latent period. In the kindling model in rats, BUM5 was more efficacious than bumetanide in potentiating the anticonvulsant effect of phenobarbital. INTERPRETATION: Our data demonstrate that the goal of designing bumetanide prodrugs that specifically target the brain is feasible and that such drugs may resolve the problems associated with using bumetanide for treatment of neurological disorders.
Asunto(s)
Encéfalo/efectos de los fármacos , Bumetanida/uso terapéutico , Epilepsia/tratamiento farmacológico , Suero/efectos de los fármacos , Inhibidores del Simportador de Cloruro Sódico y Cloruro Potásico/uso terapéutico , Potenciales de Acción/efectos de los fármacos , Animales , Animales Recién Nacidos , Encéfalo/citología , Encéfalo/metabolismo , Bumetanida/química , Bumetanida/farmacología , Convulsivantes/toxicidad , Modelos Animales de Enfermedad , Diuréticos/farmacología , Perros , Relación Dosis-Respuesta a Droga , Evaluación Preclínica de Medicamentos , Epilepsia/inducido químicamente , Humanos , Técnicas In Vitro , Ratones , Neuronas/efectos de los fármacos , Pentilenotetrazol/toxicidad , Fenobarbital/uso terapéutico , Ratas , Ratas Sprague-Dawley , Ratas Wistar , Inhibidores del Simportador de Cloruro Sódico y Cloruro Potásico/química , Inhibidores del Simportador de Cloruro Sódico y Cloruro Potásico/farmacología , Especificidad de la Especie , Factores de TiempoRESUMEN
Network activity in the brain is associated with a transient increase in extracellular K(+) concentration. The excess K(+) is removed from the extracellular space by mechanisms proposed to involve Kir4.1-mediated spatial buffering, the Na(+)/K(+)/2Cl(-) cotransporter 1 (NKCC1), and/or Na(+)/K(+)-ATPase activity. Their individual contribution to [K(+)]o management has been of extended controversy. This study aimed, by several complementary approaches, to delineate the transport characteristics of Kir4.1, NKCC1, and Na(+)/K(+)-ATPase and to resolve their involvement in clearance of extracellular K(+) transients. Primary cultures of rat astrocytes displayed robust NKCC1 activity with [K(+)]o increases above basal levels. Increased [K(+)]o produced NKCC1-mediated swelling of cultured astrocytes and NKCC1 could thereby potentially act as a mechanism of K(+) clearance while concomitantly mediate the associated shrinkage of the extracellular space. In rat hippocampal slices, inhibition of NKCC1 failed to affect the rate of K(+) removal from the extracellular space while Kir4.1 enacted its spatial buffering only during a local [K(+)]o increase. In contrast, inhibition of the different isoforms of Na(+)/K(+)-ATPase reduced post-stimulus clearance of K(+) transients. The astrocyte-characteristic α2ß2 subunit composition of Na(+)/K(+)-ATPase, when expressed in Xenopus oocytes, displayed a K(+) affinity and voltage-sensitivity that would render this subunit composition specifically geared for controlling [K(+)]o during neuronal activity. In rat hippocampal slices, simultaneous measurements of the extracellular space volume revealed that neither Kir4.1, NKCC1, nor Na(+)/K(+)-ATPase accounted for the stimulus-induced shrinkage of the extracellular space. Thus, NKCC1 plays no role in activity-induced extracellular K(+) recovery in native hippocampal tissue while Kir4.1 and Na(+)/K(+)-ATPase serve temporally distinct roles.
Asunto(s)
Hipocampo/metabolismo , Canales de Potasio de Rectificación Interna/metabolismo , Potasio/metabolismo , ATPasa Intercambiadora de Sodio-Potasio/metabolismo , Miembro 2 de la Familia de Transportadores de Soluto 12/metabolismo , Animales , Animales Recién Nacidos , Bumetanida/farmacología , Células Cultivadas , Corteza Cerebral/citología , Corteza Cerebral/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Agonistas de Aminoácidos Excitadores/farmacología , Líquido Extracelular/metabolismo , Hipocampo/citología , Hipocampo/efectos de los fármacos , Técnicas In Vitro , Masculino , Potenciales de la Membrana/efectos de los fármacos , Potenciales de la Membrana/fisiología , Oocitos , Potasio/farmacología , Ratas , Ratas Sprague-Dawley , Inhibidores del Simportador de Cloruro Sódico y Cloruro Potásico/farmacología , Xenopus laevisRESUMEN
Patterned neuronal activity such as spindle bursts in the neonatal cortex is likely to promote the maturation of cortical synapses and neuronal circuits. Previous work on cats has shown that removal of subplate neurons, a transient neuronal population in the immature cortex, prevents the functional maturation of thalamocortical and intracortical connectivity. Here we studied the effect of subplate removal in the neonatal rat primary somatosensory cortex (S1). Using intracortical EEG we show that after selective removal of subplate neurons in the limb region of S1, endogenous and sensory evoked spindle burst activity is largely abolished. Consistent with the reduced in vivo activity in the S1 limb region, we find by in vitro recordings that thalamocortical inputs to layer 4 neurons are weak. In addition, we find that removal of subplate neurons in the S1 barrel region prevents the development of the characteristic histological barrel-like appearance. Thus, subplate neurons are crucially involved in the generation of particular types of early network activity in the neonatal cortex, which are an important feature of cortical development. The altered EEG pattern following subplate damage could be applicable in the neurological assessment of human neonates.
Asunto(s)
Tipificación del Cuerpo/fisiología , Potenciales Evocados Somatosensoriales/fisiología , Vías Nerviosas/crecimiento & desarrollo , Neuronas/fisiología , Corteza Somatosensorial/crecimiento & desarrollo , Tálamo/crecimiento & desarrollo , Animales , Animales Recién Nacidos , Femenino , Masculino , Vías Nerviosas/citología , Células-Madre Neurales/fisiología , Neurogénesis/fisiología , Técnicas de Cultivo de Órganos , Ratas , Corteza Somatosensorial/citología , Tálamo/citologíaRESUMEN
In two recent papers (Rheims et al., 2009; Holmgren et al., 2010), Zilberter and coworkers argue that the well known depolarizing GABA actions that take place at the cellular and network level in the neonatal hippocampus and neocortex in vitro are pathophysiological phenomena, attributable to deficient mitochondrial energy metabolism. In their experiments, supplementing the glucose-containing solution with weak-acid substrates of mitochondrial energy metabolism (such as ß-hydroxy-butyrate, lactate, or pyruvate) abolished the spontaneous network events (giant depolarizing potentials; GDPs) and the underlying depolarizing actions of GABA. In this study, we made electrophysiological recordings of GDPs and monitored the mitochondrial membrane potential (Ψm) and intracellular pH (pH(i)) in CA3 neurons in neonatal rat hippocampal slices. Supplementing the standard physiological solution with l-lactate did not produce a change in Ψm, whereas withdrawal of glucose, in the presence or absence of l-lactate, was followed by a pronounced depolarization of Ψm. Furthermore, d-lactate (a poor substrate of mitochondrial metabolism) caused a prompt inhibition in GDP frequency which was similar to the effect of l-lactate. The suppression of GDPs was strictly proportional to the fall in pH(i) caused by weak carboxylic acids (l-lactate, d-lactate, or propionate) or by an elevated CO(2). The main conclusions of our work are that the inhibitory effect of l-lactate on GDPs is not mediated by mitochondrial energy metabolism, and that glucose at its standard 10 mm concentration is an adequate energy substrate for neonatal neurons in vitro. Notably, changes in pH(i) appear to have a very powerful modulatory effect on GDPs.
Asunto(s)
Potenciales de Acción/fisiología , Metabolismo Energético/fisiología , Hipocampo/metabolismo , Mitocondrias/metabolismo , Red Nerviosa/metabolismo , Ácido gamma-Aminobutírico/metabolismo , Animales , Animales Recién Nacidos , Glucosa/fisiología , Ácido Láctico/farmacología , Red Nerviosa/fisiología , Ratas , Ratas Wistar , Ácido gamma-Aminobutírico/fisiologíaRESUMEN
PURPOSE: To study whether respiratory alteration caused by vagal nerve stimulation (VNS) can change end-tidal carbon dioxide (EtCO2) levels. METHODS: We performed polygraphic recordings including capnographic monitoring during daytime sleep on adults with VNS therapy. RESULTS: Ten of 13 patients showed VNS-induced alterations in the frequency or amplitude of respiration. Five patients had a consistent increase in respiratory rate with a simultaneous, consistent and significant decrease (p < 0.01; 5-22%) in EtCO2 during VNS. Three subjects showed occasional decreases in EtCO2 during VNS, and two showed no clearly detectable VNS-related EtCO2 changes. CONCLUSIONS: Our findings suggest that VNS may alter brain CO2 levels through changes in respiration. Because carbon dioxide (CO2) has potent effects on various brain functions, it is possible that these transient CO2 changes may have an effect on the state transitions between interictal and preictal states.
Asunto(s)
Terapia por Estimulación Eléctrica , Epilepsias Parciales/terapia , Epilepsia Generalizada/terapia , Hipocapnia/etiología , Nervio Vago/fisiopatología , Adulto , Encéfalo/fisiopatología , Capnografía , Electroencefalografía , Femenino , Humanos , Hipocapnia/fisiopatología , Masculino , Persona de Mediana Edad , Polisomnografía , Ventilación Pulmonar/fisiología , Volumen de Ventilación Pulmonar/fisiología , Resultado del TratamientoRESUMEN
To understand spoken language, the human brain must have fast mechanisms for the representation and identification of speech sounds. Stimulus-induced synchronization of neural activity at gamma frequencies (20-80 Hz), occurring in humans at 200-300 msec from stimulus onset, has been suggested to be a possible mechanism for neural object representation. Auditory and visual stimuli also evoke an earlier (peak <100 msec) gamma oscillation, but its dependence on high-level stimulus parameters and, thereby, its involvement in object representation has remained unclear. Using whole-scalp magnetoencephalography, we show here that responses evoked by speech and non-speech sounds differed in the gamma-frequency but not in the low-frequency (0.1-20 Hz) band as early as 40-60 msec from stimulus onset. The gamma-band responses to the speech sound peaked earlier in the left than in the right hemisphere, whereas those to the non-speech sound peaked earlier in the right hemisphere. For the speech sound, there was no difference in the response amplitude between the hemispheres at low (20-45 Hz) gamma frequencies, whereas for the non-speech sound, the amplitude was larger in the right hemisphere. These results suggest that evoked gamma-band activity may indeed be sensitive to high-level stimulus properties and may hence reflect the neural representation of speech sounds. Consequently, speech-specific neuronal processing may commence no later than 40-60 msec from stimulus onset, possibly in the form of activation of language-specific memory traces.