RESUMEN
Self-incompatibility (SI) enables flowering plants to discriminate between self- and non-self-pollen. In Brassica, SI is controlled by the highly polymorphic S locus. The recently identified male determinant, termed SP11 or SCR, is thought to be the ligand of S receptor kinase, the female determinant. To examine functional and evolutionary properties of SP11, we cloned 14 alleles from class-I S haplotypes of Brassica campestris and carried out sequence analyses. The sequences of mature SP11 proteins are highly divergent, except for the presence of conserved cysteines. The phylogenetic trees suggest possible co-evolution of the genes encoding the male and female determinants.
Asunto(s)
Brassica/genética , Glicoproteínas/genética , Proteínas de Plantas/genética , Polen/genética , Alelos , Secuencia de Aminoácidos , Mapeo Cromosómico , Clonación Molecular , ADN Complementario/química , ADN Complementario/genética , ADN de Plantas/genética , Electroforesis en Gel de Campo Pulsado , Evolución Molecular , Variación Genética , Haplotipos , Datos de Secuencia Molecular , Filogenia , Alineación de Secuencia , Análisis de Secuencia de ADN , Homología de Secuencia de AminoácidoRESUMEN
A 32-year-old female presented with a hypothalamic hamartoma associated with a dural arteriovenous fistula (DAVF) of the left transverse-sigmoid sinus. She complained of pulsatile tinnitus in the left retroauricular region and infertility. Endocrinological examination revealed that the luteinizing hormone reaction to luteinizing hormone-releasing hormone was exceedingly high. Magnetic resonance imaging demonstrated a distinct mass occupying the suprasellar cistern and protruding into the third ventricle. Left carotid angiography demonstrated a DAVF of the left transverse-sigmoid sinus, which was treated by embolization. Histological examination of a biopsy specimen of the tumor revealed a hamartoma. She was free from pulsatile tinnitus after treatment for the DAVF.