[The vaccinal process after the peroral immunization of lambs with the Salmonella typhimurium 274 strain]. / Vaktsinal'nyi protsess posle peroral'noi immunizatsii iagniat shtammom Salmonella typhimurium 274.

After oral immunization of lambs with S. typhimurium vaccine strain 274 these bacteria were characterized by low adhesion to the epithelial surface of the ileum, cecum, and colon, by very insignificant invasion into intestinal epithelium and then into lamina propria of the intestinal mucosa, mesenteric lymph nodes and the blood; the blood stream carried these bacteria further to the liver, spleen and other organs. Bacteria of strain 274 produced no damages, did not multiply and only persisted for 7-13 days in some lambs. No inflammation developed in response to oral immunization of the animals. The developing immunomorphological reaction presented as just a negligible increase in lymphocyte count in the intestinal epithelium, blast transformation and plasmatization in lymphatic follicles of Peyer's patches, hyperplastic mesenteric lymph nodes, blast and lymphocyte recirculation, moderate hyperplasia of white splenic pulp and insignificant lymphoid infiltration of portal interlayers of the liver.

[A morphological evaluation of the protective properties of noninvasive recombinant strains of Shigella]. / Morfologicheskaia otsenka protektivnosti neinvazivnykh rekombinantnykh shtammov shigell.

In 2-3 weeks after the oral immunization of rabbits, made in one or two administrations, with attenuated two-marker S. dysenteriae 1 strain VS-12 and recombinant S. dysenteriae VS-12/S. sonnei NR-18 and S. flexneri y433/S. sonnei NR-18 pronounced immunological reaction developed in the mucous membrane of the small intestine: blast transformation follicles of Peyer's patches, an increase in the number of lymphoblasts and plasmocytes in the cupolae of follicles and in intestinal villi, and an increase in the number of lymphocytes and macrophages in the intestinal epithelium with their release into the lumen of the intestine after challenge with virulent shigellae. The protective potency of these recombinants after challenge with massive doses of virulent shigellae was found to be high, which was shown by quantitative evaluation of the decrease of adhesion, invasiveness and cytotoxicity, suppression of epithelial lesions and development of inflammation in the intestinal mucosa.