RESUMEN
Murraya koenigii (MK) relates to the Rutaceae family and has many health benefits. To date, over eighty-eight carbazole alkaloids along with terpenoids, and other nutrients have been identified from different parts of this plant. This review presents accumulated information regarding the role of MK and its constituents in the prevention/treatment of cancer. Literature survey revealed that MK and its constituents target multiple deranged pathways associated with apoptosis, growth (JAK-STAT, mTOR), and cell cycle in a variety of cancerous cell lines (colon, lung, liver, skin, prostate, breast, etc.) and few animal models. Thus, the present review highlights the anticancer mechanism of MK and its phytoconstituents, and further future perspectives. The ameliorating effects of MK and its phytoconstituents against various cancers warrant its multi-institutional clinical trials as soon as possible. The prospects of relatively cheaper cancer drugs could then be brighter, particularly for the socio-economically feebler cancer patients of the world.
Asunto(s)
Alcaloides , Murraya , Alcaloides/farmacología , Animales , Humanos , Extractos Vegetales/farmacología , Extractos Vegetales/uso terapéutico , Hojas de la Planta , ÁrbolesRESUMEN
The present study assessed the effect of selenium (Se) supplementation on Venlafaxine hydrochloride (VH)-induced testicular toxicity. Mice were segregated into Group I (C), Group II (0.5 ppm Se), Group III (VH at a dose 60 mg/kg b.w.) and Group IV (Se was given as per Group II, and VH was given as per Group III). After 10 weeks, sperm parameters, histology, sperm cell counts, antioxidants activities, apoptotic proteins and molecular analysis of testicular tissue were evaluated. Group III had significantly lower sperm concentration (from 2.17 ± 0.28 to 1.04 ± 0.22) and sperm motility (from 68.04 ± 5.5 to 21.47 ± 5.21), and showed an extensive vacuolisation in the germinal epithelium, abnormal basement membrane, and reduced germ cell number as compared to Group I. However, selenium supplementation in Group IV substantially increased sperm concentration (1.47 ± 0.48) and motility (33.27 ± 8.66), improved the histoarchitecture and repopulated the germ cells as observed by raised numbers of spermatogonia, spermatocytes, round spermatids and elongated spermatids contrasted to Group III. Group IV also showed a noteworthy decreased ROS, LPO levels, as well as expressions of Bax, caspase-9, and caspase-3 and increased the SOD, CAT, GPx, and GSH activities as well the expression of Bcl-2 as compared to Group III. This effect was further supported by FTIR analysis for nucleic acids. Thus, selenium supplementation showed significant protection against VH-induced testicular toxicity.
Asunto(s)
Selenio , Animales , Antioxidantes/metabolismo , Antioxidantes/farmacología , Apoptosis , Humanos , Masculino , Ratones , Estrés Oxidativo , Selenio/farmacología , Motilidad Espermática , Testículo/metabolismo , Clorhidrato de Venlafaxina/metabolismo , Clorhidrato de Venlafaxina/farmacologíaRESUMEN
Being a vital micronutrient, along with a trace element, selenium (Se) protects the cells from oxidative stress (OS) in the form of selenoproteins. Bisphenol A (BPA) is a xeno-oestrogenic compound that adversely affects the spermatogenesis process by inducing oxidative stress, which ultimately leads to male infertility. Therefore, it is hypothesised that Se could protect against BPA-induced OS, and further germ cell death by modifying mitogen-activated protein kinase (MAPK) signalling. Male Balb/c mice were divided into four groups: Group I (C) (0.2 ppm Se), Group II (Se) (0.5 ppm Se), Group III (BPA) (0.2 ppm Se, and BPA = 1 mg/kg orally) and Group IV (Se + BPA) (0.5 ppm Se, and BPA = 1 mg/kg bodyweight orally). Results indicated that BPA-treated animals demonstrated a marked decrease in antioxidant enzyme activities (superoxide dismutase, catalase, redox ratio), a marked elevation in the expressions of stress-activated kinases (c-Jun NH2-terminal kinase (JNK), extracellular signal-regulated kinase (ERK) and p38) and the expressions of pro-apoptotic markers (caspase-9, caspase-8 and caspase-3). However, Se supplementation considerably restored the antioxidant enzyme activities and lowered the expressions of stress-activated kinases, which further down-regulated the apoptosis. Thus, Se supplementation demonstrated to be effective against BPA provoked testicular damage.
Asunto(s)
Selenio , Animales , Apoptosis , Compuestos de Bencidrilo , Proteínas Quinasas JNK Activadas por Mitógenos/metabolismo , Masculino , Ratones , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Estrés Oxidativo , Fenoles , Selenio/farmacología , Testículo/metabolismo , Proteínas Quinasas p38 Activadas por Mitógenos/metabolismoRESUMEN
The present study aimed to examine the chemoprotective effect of Hydroethanolic Murraya koenigii leaves extract (HEMKLE) on murine skin carcinogenesis model. For the study, male LACA mice divided into four groups (n = 15 per group). Group I (Control), Group II (DMBA/TPA), Group III (HEMKLE), and Group IV (HEMKLE + DMBA/TPA). Skin tumors were induced in Group II (DMBA/TPA) and Group IV (HEMKLE + DMBA/TPA) by topical application of 7, 12 dimethylbenz[a]anthracene (DMBA) [500 nmol/100 µL of acetone, twice a week for two weeks] and 12-O-tetradecanoyl phorbol-13-acetate (TPA) [1.7 nmol/100 µL of acetone, twice a week for eighteen weeks] and HEMKLE (200 mg/kg b. w.) was administered orally (instilled by oral gavage). The chemoprotective response of HEMKLE was evident by inhibition in tumor incidence, mean tumor volume, mean tumor burden, total number of tumors, and tumor size in Group IV (HEMKLE + DMBA/TPA) when compared to Group II (DMBA/TPA). HEMKLE administration also decreased the reactive oxygen species (ROS) and lipid peroxidation (LPO) levels and increased the antioxidants enzyme activities in Group IV (HEMKLE + DMBA/TPA) when compared to Group II (DMBA/TPA) that suggests its antioxidant potential. HEMKLE administration also increased the mRNA and protein expression of caspase-9 and caspase-3 and decreased the mRNA and protein expression of Bcl-2 in Group IV (HEMKLE + DMBA/TPA) when compared to Group II (DMBA/TPA) that suggest its apoptosis-inducing effect on DMBA/TPA induced skin carcinogenesis.
Asunto(s)
Murraya , Neoplasias Cutáneas , 9,10-Dimetil-1,2-benzantraceno/toxicidad , Animales , Carcinogénesis , Ratones , Extractos Vegetales , Hojas de la Planta , Neoplasias Cutáneas/inducido químicamente , Neoplasias Cutáneas/prevención & controlRESUMEN
Doxorubicin, a chemotherapeutic drug, is known to disrupt the normal spermatogenesis by excess oxidative stress. The present study describes the curative effects of dietary supplemented selenium on doxorubicin-induced testicular damage in mice. Four groups were included in the study: Group I(C), Group II (Se-0.5 ppm/kg diet), Group III (Dox-3mg/kg body weight i.p.) and Group IV (Se + Dox). We analysed microscopic sperm parameters, histopathology, testicular germ cell kinetics, oxidative stress levels, antioxidant levels and mRNA expression studies of apoptotic and stress response markers. Sperm parameters were significantly reduced in doxorubicin-treated group. Moreover, mice treated with doxorubicin showed an elevation in oxidative stress markers as well as decreased redox ratio, and antioxidant levels were observed in Group III (Dox). However, selenium supplementation ameliorated the damage incurred by doxorubicin, by improving sperm parameters, antioxidant levels and histoarchitecture of mice testes, and decreased the oxidative stress levels. Selenium administration also reduced the levels of apoptotic caspases and stress-activated kinases in Group IV (Se + Dox) when compared to Group III (Dox). In conclusion, selenium exhibits the curative effect against doxorubicin-induced testicular damage in mice by attenuating stress conditions and associated apoptosis.
Asunto(s)
Preparaciones Farmacéuticas , Selenio , Animales , Antioxidantes/metabolismo , Antioxidantes/farmacología , Apoptosis , Suplementos Dietéticos , Doxorrubicina/toxicidad , Masculino , Ratones , Estrés Oxidativo , Preparaciones Farmacéuticas/metabolismo , Selenio/farmacología , Testículo/metabolismoRESUMEN
Bisphenol A (BPA) is a well-known endocrine disruptor that imposees adverse effects on male fertility via interacting with germ cells of testis. Objectives of present study were to investigate the possible protective effects of hydroethanolic Murraya koenigii leaves extract (HEMKLE) against BPA-induced testicular damage and apoptosis in mice. Male Balb/c mice were divided into four different groups: Group I (control), Group II (HEMKLE), Group III (BPA) and Group IV (HEMKLE + BPA). Group III (BPA) showed significant decrease in sperm parameters, germ cell number along with increased lipid peroxidation (LPO) and reactive oxygen species (ROS). A significant decrease in antioxidant enzymes activity was also observed in Group III (BPA) animals. mRNA expression study revealed significant decrease in the expression of Bcl-2 and increase in expressions of caspase-9 and caspase-3, thus clearly demonstrate BPA-induced apoptosis. In addition, HEMKLE co-administration to BPA-treated mice showed a significant increase in sperm parameters, germ cell number, decreased levels of LPO and ROS, increased antioxidant enzymes activity in Group IV (HEMKLE + BPA). Also, mRNA expression study showed a significant increase in Bcl-2 and decrease in caspase-9 and caspase-3 gene expressions in Group IV (HEMKLE + BPA). Thus, the present study suggests that HEMKLE intervention provides protection against BPA-induced oxidative stress and apoptosis.
Asunto(s)
Compuestos de Bencidrilo/toxicidad , Disruptores Endocrinos/toxicidad , Murraya/química , Fenoles/toxicidad , Extractos Vegetales/farmacología , Testículo/efectos de los fármacos , Animales , Apoptosis/efectos de los fármacos , Etanol/química , Humanos , Infertilidad Masculina/inducido químicamente , Infertilidad Masculina/prevención & control , Masculino , Ratones , Modelos Animales , Estrés Oxidativo/efectos de los fármacos , Extractos Vegetales/aislamiento & purificación , Hojas de la Planta/química , Espermatozoides/efectos de los fármacos , Espermatozoides/patología , Testículo/patología , Agua/químicaRESUMEN
Bisphenol A (BPA), an organic synthetic compound and endocrine disruptor, which majorly cause deleterious effects on male reproductory system. Fenugreek (Trigonella foenum-graecum), associated with Leguminosae family is used as a herbal medicine with potent antioxidant properties. The present study was aimed to scrutinise the preventative role of fenugreek seeds aqueous extract (FSEt) on BPA-induced testicular damage in mice. Study included four different groups of male Balb/c mice: contol (C), fenugreek (FSEt), bisphenol A (BPA) and fenugreek + bisphenol A (FSEt + BPA). After two months of treatment, assessment of sperm parameters, antioxidant defence system, histopathological studies, germ cell count and gene expression of intrinsic apoptotic pathway were carried out. Administration of FSEt improved the damage caused by BPA as indicated by improved sperm parameters. FSEt-administered mice showed improvement in the histoarchitecture compared with BPA-administered animals. In addition, fenugreek treatment showed reduced levels of malondialdehyde and elevated levels of antioxidant enzymes. Expression studies of apoptotic markers revealed a significant decrease in the expression of Bcl-2 and significant increase in caspase-9 and caspase-3. However, FSEt restored the deleterious effects caused by BPA. The current findings plausibly might have promising protective role against BPA-induced testicular damage.