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1.
J Exp Bot ; 57(9): 2049-59, 2006.
Artículo en Inglés | MEDLINE | ID: mdl-16720613

RESUMEN

Plants have developed several strategies for coping with phosphorus (P) deficiency. However, the details of the regulation of gene expression of adaptations to low P are still unclear. Using a cDNA microarray, transcriptomic analyses were carried out of the rice genes regulated by P deficiency and P re-supply to P-deficient plants. The OsPI1 gene, which was isolated as the most significant up-regulated gene under -P conditions, was also the most significant down-regulated gene following P re-supply. Many starch metabolism-related genes, as well as several genes for P(i)-liberating enzymes, were up-regulated by -P treatment, suggesting a homeostatic contribution to the P(i) concentration in leaf tissues. mRNAs for glucanases were also induced by P re-supply: these are suspected to play a role in loosening the cell wall compounds. Most of the genes up-regulated by -P treatment were down-regulated by P re-supply, suggesting that their responses were specific to -P conditions. Conversely, the number of genes up-regulated by P re-supply was also larger following P re-supply than in the -P condition. It is proposed that the genes up-regulated by P re-supply play an important role in P acquisition by P-deficient plants.


Asunto(s)
Oryza/metabolismo , Fósforo/metabolismo , Hojas de la Planta/metabolismo , Perfilación de la Expresión Génica , Regulación de la Expresión Génica de las Plantas , Genes de Plantas , Análisis de Secuencia por Matrices de Oligonucleótidos
2.
Mol Biol Evol ; 21(10): 1855-70, 2004 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-15215322

RESUMEN

We obtained 32K full-length cDNA sequence data from the rice full-length cDNA project and performed a homology search against NCBI GenBank data. We have also searched homologs of Arabidopsis and other plants' genes with the databases. Comparative analysis of calcium ion transport proteins revealed that the genes specific for muscle and nerve calcium signal transduction systems (VDCC, IP3 receptor, ryanodine receptor) are very different in animals and plants. In contrast, Ca elements with basic functions in cell responses (CNGC, iGlu receptor, Ca(2+)ATPase, Ca2+/Na(+)-K+ ion exchanger) are basically conserved between plants and animals. We also performed comparative analyses of calcium ion binding and/or controlling signal transduction proteins. Many genes specific for muscle and nerve tissue do not exist in plants. However, calcium ion signal transduction genes of basic functions of cell homeostasis and responses were well conserved; plants have developed a calcium ion interacting system that is more direct than in animals. Many species of plants have specifically modified calcium ion binding proteins (CPK, CRK), Ca2+/phospholipid-binding domains, and calcium storage proteins.


Asunto(s)
Arabidopsis/genética , Calcio/metabolismo , Oryza/genética , Transducción de Señal/genética , Animales , Arabidopsis/metabolismo , Canales de Calcio/genética , Canales de Calcio/metabolismo , Proteínas de Unión al Calcio/genética , Proteínas de Unión al Calcio/metabolismo , Cisteína Endopeptidasas/genética , Cisteína Endopeptidasas/metabolismo , ADN Complementario , Bases de Datos de Proteínas , Endocitosis/genética , Endocitosis/fisiología , Humanos , NADPH Oxidasas/genética , NADPH Oxidasas/metabolismo , Oryza/metabolismo , Estructura Secundaria de Proteína , Transducción de Señal/fisiología
3.
Biosci Biotechnol Biochem ; 67(2): 347-53, 2003 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-12728997

RESUMEN

A cDNA encoding a predicted 15-kDa protein was earlier isolated from sugar-induced genes in rice embryos (Oryza sativa L.) by cDNA microarray analysis. Here we report that this cDNA encodes a novel Ca2+-binding protein, named OsSUR1 (for Oryza sativa sugar-up-regulated-1). The recombinant OsSUR1 protein expressed in Escherichia coli had 45Ca2+-binding activity. Northern analysis showed that the OsSUR1 gene was expressed mainly in the internodes of mature plants and in embryos at an early stage of germination. Expression of the OsSUR1 gene was induced by sugars that could serve as substrates of hexokinase, but expression was not repressed by Ca2+ signaling inhibitors, calmodulin antagonists and inhibitors of protein kinase or protein phosphatase. These results suggested that Os-SUR1 gene expression was stimulated by a hexokinase-dependent pathway not mediated by Ca2+.


Asunto(s)
Proteínas de Unión al Calcio/genética , Proteínas de Unión al Calcio/metabolismo , Metabolismo de los Hidratos de Carbono , ADN Complementario/genética , ADN de Plantas/genética , Hexoquinasa/metabolismo , Secuencias de Aminoácidos , Secuencia de Aminoácidos , Calcio/química , Calcio/metabolismo , Isótopos de Calcio , Carbohidratos/farmacología , ADN Complementario/aislamiento & purificación , ADN de Plantas/aislamiento & purificación , Escherichia coli/metabolismo , Regulación de la Expresión Génica de las Plantas/genética , Regulación de la Expresión Génica de las Plantas/fisiología , Datos de Secuencia Molecular , Oryza/embriología , Oryza/genética , Oryza/crecimiento & desarrollo , Oryza/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Alineación de Secuencia , Homología de Secuencia de Aminoácido , Transducción de Señal
4.
Plant J ; 30(1): 83-94, 2002 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-11967095

RESUMEN

To acquire Fe from soil, graminaceous plants secrete mugineic acid family phytosiderophores (MAs) from their roots. The secretion of MAs increases in response to Fe deficiency, and shows a distinct diurnal rhythm. We used a microarray that included 8987 cDNAs of rice EST clones to examine gene expression profiles in barley roots during Fe-deficiency stress. Approximately 200 clones were identified as Fe-deficiency-inducible genes, of which seven had been identified previously. In order to meet the increased demand for methionine to produce MAs, Fe-deficiency enhances the expression of genes that participate in methionine synthesis, as well as recycling methionine through the Yang cycle. Of these 200 genes, approximately 50 exhibited different transcription levels in Fe-deficient roots at noon and at night. Northern blot analysis of time course experiments confirmed that five of these genes exhibited a diurnal change in their level of expression. The diurnal changes in the expression of these genes suggest that polar vesicle transport is involved in the diurnal secretion of MAs.


Asunto(s)
Ácido Azetidinocarboxílico/análogos & derivados , ADN Complementario/genética , Perfilación de la Expresión Génica , Hordeum/genética , Deficiencias de Hierro , Análisis de Secuencia por Matrices de Oligonucleótidos/métodos , Ácido Azetidinocarboxílico/metabolismo , Transporte Biológico , Northern Blotting , Ritmo Circadiano , Etiquetas de Secuencia Expresada , Hordeum/efectos de los fármacos , Hordeum/metabolismo , Hierro/farmacología , Metionina/biosíntesis , Oryza/genética , Epidermis de la Planta/genética , Epidermis de la Planta/metabolismo , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/genética , Raíces de Plantas/metabolismo , Sideróforos/metabolismo
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