Asunto(s)
Cryptomeria , Inmunoterapia Sublingual , Alérgenos , Apoptosis , Linfocitos T CD4-Positivos , Humanos , Extractos Vegetales/farmacología , Polen , Linfocitos TRESUMEN
Sublingual immunotherapy (SLIT) with Japanese cedar (JCe) pollinosis was expected to be effective for Japanese cypress (JCy) pollinosis. However, only a half of JCy pollinosis patients clinically improved. Therefore, we examined the immunological effect of SLIT for JCy pollinosis. Peripheral blood mononuclear cells (PBMCs) from patients with JCe and JCy pollinosis who did and did not receive SLIT were incubated with Cry j 1, Cha o 1 and Cha o 3 antigens. Basophil activation test (BAT) were performed. Production of IL-5 and IL-17 induced by antigens was inhibited in the SLIT group. Cry j 1-specific production of IL-10 was increased, and serum Cry j 1-specific IgE and -IgG4 were elevated. However, Cha o 1- or Cha o 3-specific production of IL-10 and specific IgG4 was not increased. Antigens-specific BAT did not decrease after SLIT. New SLIT with JCe and JCy is needed for patients with combined JCe and JCy pollinosis.
Asunto(s)
Leucocitos Mononucleares/inmunología , Rinitis Alérgica Estacional/terapia , Inmunoterapia Sublingual/métodos , Adulto , Antígenos de Plantas/inmunología , Prueba de Desgranulación de los Basófilos , Células Cultivadas , Chamaecyparis/inmunología , Cryptomeria/inmunología , Citocinas/metabolismo , Femenino , Humanos , Inmunoglobulina E/metabolismo , Inmunoglobulina G/metabolismo , Masculino , Persona de Mediana Edad , Extractos Vegetales/inmunología , Proteínas de Plantas/inmunología , Polen/inmunología , Estudios Prospectivos , Rinitis Alérgica Estacional/inmunologíaAsunto(s)
Alérgenos/efectos adversos , Antígenos de Plantas/efectos adversos , Cryptomeria/química , Glucósidos/farmacología , Glucósidos/uso terapéutico , Inmunoterapia/métodos , Lípido A/farmacología , Lípido A/uso terapéutico , Proteínas de Plantas/efectos adversos , Polen/efectos adversos , Rinitis Alérgica Estacional/etiología , Rinitis Alérgica Estacional/terapia , Receptor Toll-Like 4/agonistas , Animales , Antígenos de Plantas/inmunología , Células Cultivadas , Cryptomeria/inmunología , Modelos Animales de Enfermedad , Humanos , Japón/epidemiología , Leucocitos Mononucleares/efectos de los fármacos , Leucocitos Mononucleares/metabolismo , Ratones , Ratones Endogámicos BALB C , Proteínas de Plantas/inmunología , Polen/inmunología , Rinitis Alérgica Estacional/epidemiología , Resultado del TratamientoRESUMEN
IL-25 likely has vital roles in initiating and activating type-2 immune responses in AR. We hypothesized that the molecules produced IL-25 by allergen-producing organisms such as JC is involved in the pathogenesis of AR. Participants included 13 patients with Japanese cedar pollinosis and 10 HCs. We measured the IL-25 protein concentration in nasal secretions and in culture supernatants of PNECs. NHBE cells were stimulated with pharmacological and immunological agents and JC. The IL-25 concentration in nasal secretions was significantly higher in patients with Japanese cedar pollinosis than in HCs. JC stimulated IL-25 production from PNECs. TNF-α, IL-4, and IL-13 significantly enhanced JC-induced IL-25 production; their activation by serine proteases was sufficient to enhance IL-25 production. Furthermore, the NADPH oxidase activity, including JC enhanced IL-25 production. A better understanding of JC-induced IL-25 production by epithelial cells may allow the development of novel therapeutic and preventive strategies for Japanese cedar pollinosis.