RESUMEN
BACKGROUND/AIMS: Although many regimens, including quadruple, sequential, and concomitant treatment, are used and recommended as first-line or rescue therapies for Helicobacter pylori infection, eradication rates are still below 90% in intention-to-treat analyses. Treatment protocols with substantially high eradication rates and low antibiotic resistance are needed. In this study, we investigated the efficacy of high-dose dual therapy as first-line treatment in a Turkish population. MATERIALS AND METHODS: All patients underwent upper gastrointestinal endoscopy for the initial H. pylori status because of dyspeptic symptoms. All patients received a 14-day, high-dose dual therapy comprising rabeprazole (20 mg t.i.d.) and amoxicillin (1 g t.i.d.) for H. pylori eradication. H. pylori stool antigen tests of eradication were administered to all participants at least 4 weeks after the completion of the treatment. RESULTS: The high-dose dual therapy demonstrated a 91.3% rate of successful eradication of H. pylori infection. Per-protocol success was 94.4% among female patients (n=51) and 89.6% among male patients (n=86); in terms of gender, the differences were not significant (p=0.310). No side effects were observed during the study in any patient. Six other patients did not take adequate doses of the treatment protocol. CONCLUSION: High-dose dual therapy with rabeprazole and amoxicillin was highly effective and well tolerated as a first-line therapy for H. pylori eradication.
Asunto(s)
Amoxicilina/administración & dosificación , Antibacterianos/administración & dosificación , Infecciones por Helicobacter/tratamiento farmacológico , Helicobacter pylori , Rabeprazol/administración & dosificación , Adolescente , Adulto , Anciano , Quimioterapia Combinada , Femenino , Infecciones por Helicobacter/microbiología , Humanos , Masculino , Persona de Mediana Edad , Resultado del Tratamiento , Turquía , Adulto JovenRESUMEN
Porcine pancreatic lipase was covalently immobilized on polyvinyl alcohol using adipoyl dichloride as a cross-linker. The effect of pre-treatment of lipase previously with various types of oils on immobilization efficiency was investigated. The increment in immobilization efficiency was observed after pre-treatment of lipases with oils. The highest immobilization efficiency obtained was 20% (v/v) for olive oil pre-treated lipase, which was 8 times higher than that of non-pretreated immobilized lipase. Immobilized lipase had better stability and had some advantages in comparison with free enzyme.