RESUMEN
Crocus sativus L., a perennial plant grown mainly around the Mediterranean and Iran, has many medicinal properties including anti-inflammatory, anti-depressive and cancer preventing properties. Aqueous herbal extracts may affect the activity of Phase I and II enzymes involved in xenobiotic metabolism. The present study was designed to determine whether C. sativus infusion alters the activity of CYP1A2, CYP2A6, XO and NAT2 enzymes in humans. Thirty-four healthy volunteers consumed infusion prepared from C. sativus stigmata for six days. Enzyme phenotyping was assessed in saliva and urine using caffeine metabolite ratios as follows: CYP1A2: 17X/137Χ (saliva) and CYP1A2: (AFMU+1U+1X)/17U, CYP2A6: 17U/(17U + 17X), XO: 1U/(1U+1X) and NAT2: AFMU/(AFMU+1U+1X) (urine). Following C. sativus intake, CYP1A2 index was reduced by â¼13.7% in saliva (before: 0.51⯱â¯0.22, after: 0.44⯱â¯0.14; pâ¯=â¯0.002) and â¼6.0% in urine (before: 3.81⯱â¯1.20, after: 3.58⯱â¯0.92; pâ¯=â¯0.054). CYP1A2 index was significantly reduced only in males (saliva, before: 0.65⯱â¯0.22, after: 0.51⯱â¯0.16; pâ¯=â¯0.0001; urine, before: 4.53⯱â¯1.19, after: 4.03⯱â¯0.87; pâ¯=â¯0.017) suggesting sexual dimorphism in CYP1A2 inhibition. There was no effect of C. sativus intake on CYP2A6, XO or NAT2 indices. Short-term consumption of C. sativus infusion is unlikely to result in significant herb-drug interactions involving the enzymes studied, with the exception of potential herb-CYP1A2 substrate interaction in males.
Asunto(s)
Crocus/metabolismo , Sistema Enzimático del Citocromo P-450/metabolismo , Regulación Enzimológica de la Expresión Génica/efectos de los fármacos , Hígado/enzimología , Adulto , Carotenoides/química , Femenino , Voluntarios Sanos , Interacciones de Hierba-Droga , Humanos , Hígado/metabolismo , Masculino , Persona de Mediana Edad , Adulto JovenRESUMEN
Sideritis scardica(S. scardica) is an endemic plant of the Balkan Peninsula traditionally used as herbal tea for inflammation and gastric disorders. Aqueous herbal extracts may affect the activity of Phase I and II enzymes involved in xenobiotic metabolism. The purpose of the present study was to determine whether S. scardica decoction alters the activity of CYP1A2, CYP2A6, XO, NAT2 and UGT1A1/1A6 enzymes in humans. Fourteen healthy subjects consumed S. scardica decoction for six days. Enzyme phenotyping was assessed in saliva and urine using caffeine and paracetamol metabolite ratios as follows: CYP1A2: 17X/137X (saliva) and (AFMU+1U+1X)/17U, CYP2A6: 17U/(17U + 17X), XO: 1U/(1U+1X), NAT2: AFMU/(AFMU+1U+1X) and UGT1A1/1A6: glucuronidated/total paracetamol (urine). After S. scardica intake, CYP1A2 index was reduced by â¼16% and â¼8% in saliva (before: 0.54⯱â¯0.18, after: 0.46⯱â¯0.09; pâ¯=â¯0.08) and urine (before: 3.59⯱â¯0.52, after: 3.67⯱â¯0.78; pâ¯=â¯0.12), respectively. CYP2A6 index was significantly reduced only in males (before: 0.76⯱â¯0.08, after: 0.67⯱â¯0.07; pâ¯=â¯0.004), suggesting sexual dimorphism in CYP2A6 inhibition. There was no effect of Sideritis scardica treatment on XO, NAT2 or UGT1A1/1A6 indices. Usual consumption of the aerial parts of S. scardica decoction is unlikely to result in herb-drug interactions involving the enzymes studied, with the exception of potential herb-CYP2A6 substrate interaction in males.