RESUMEN
Long chain polyunsaturated fatty acids (LCPUFA)seem to be the most trophic macronutrients in inducing intestinal adaptation in adult short bowel syndrome (SBS), although their effects on intestinal adaptation in infants with SBS remain unknown.It is hypothesized that a high fat diet enriched with n-3 LCPUFA derived from fish oil (FO) will increase intestinal adaptation compared with a diet dominated by n-6 PUFA from corn oil (CO) in weanling SBS rats after massive ileocecal resection (ICR). Twenty-day-old rats were sorted into four groups, CO-sham, FO-sham,CO-ICR, and FO-ICR groups, and fed ad lib with the CO or FO diet, respectively, for 7 d after sham or ICR surgery. Compared with CO-ICR rats, FO-ICR rats consumed less diet per gram of weight gain, had less diarrhea and fecal fat excretion, and demonstrated a tendency toward better weight gain. The mucosal mass, DNA and RNA levels of the colon and RNA levels of the distal jejunum, and the colonic mucosal area (%) were significantly higher in FO-ICR rats than in CO-ICR rats. These results suggest that the beneficial effect of dietary FO is associated with better adaptation in the colon in weanling rats after ICR.
Asunto(s)
Adaptación Fisiológica/fisiología , Modelos Animales de Enfermedad , Aceites de Pescado/farmacología , Mucosa Intestinal/metabolismo , Intestinos/cirugía , Síndrome del Intestino Corto/fisiopatología , Análisis de Varianza , Anastomosis Quirúrgica , Animales , Diarrea/etiología , Suplementos Dietéticos , Heces/química , Mucosa Intestinal/patología , Intestinos/patología , Masculino , Ratas , Ratas Sprague-DawleyRESUMEN
Multiwalled carbon nanotubes (MWCNTs) exhibit physical properties that render them ideal candidates for application as noninvasive mediators of photothermal cancer ablation. Here, we demonstrate that use of MWCNTs to generate heat in response to near-infrared radiation (NIR) results in thermal destruction of kidney cancer in vitro and in vivo. We document the thermal effects of the therapy through magnetic resonance temperature-mapping and heat shock protein-reactive immunohistochemistry. Our results demonstrate that use of MWCNTs enables ablation of tumors with low laser powers (3 W/cm(2)) and very short treatment times (a single 30-sec treatment) with minimal local toxicity and no evident systemic toxicity. These treatment parameters resulted in complete ablation of tumors and a >3.5-month durable remission in 80% of mice treated with 100 microg of MWCNT. Use of MWCNTs with NIR may be effective in anticancer therapy.