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1.
Genome Biol ; 25(1): 61, 2024 02 27.
Artículo en Inglés | MEDLINE | ID: mdl-38414075

RESUMEN

BACKGROUND: Tartary buckwheat, Fagopyrum tataricum, is a pseudocereal crop with worldwide distribution and high nutritional value. However, the origin and domestication history of this crop remain to be elucidated. RESULTS: Here, by analyzing the population genomics of 567 accessions collected worldwide and reviewing historical documents, we find that Tartary buckwheat originated in the Himalayan region and then spread southwest possibly along with the migration of the Yi people, a minority in Southwestern China that has a long history of planting Tartary buckwheat. Along with the expansion of the Mongol Empire, Tartary buckwheat dispersed to Europe and ultimately to the rest of the world. The different natural growth environments resulted in adaptation, especially significant differences in salt tolerance between northern and southern Chinese Tartary buckwheat populations. By scanning for selective sweeps and using a genome-wide association study, we identify genes responsible for Tartary buckwheat domestication and differentiation, which we then experimentally validate. Comparative genomics and QTL analysis further shed light on the genetic foundation of the easily dehulled trait in a particular variety that was artificially selected by the Wa people, a minority group in Southwestern China known for cultivating Tartary buckwheat specifically for steaming as a staple food to prevent lysine deficiency. CONCLUSIONS: This study provides both comprehensive insights into the origin and domestication of, and a foundation for molecular breeding for, Tartary buckwheat.


Asunto(s)
Fagopyrum , Domesticación , Fagopyrum/genética , Perfilación de la Expresión Génica , Estudio de Asociación del Genoma Completo , Genómica , Filogenia
2.
Mol Plant ; 16(9): 1427-1444, 2023 09 04.
Artículo en Inglés | MEDLINE | ID: mdl-37649255

RESUMEN

Common buckwheat (Fagopyrum esculentum) is an ancient crop with a world-wide distribution. Due to its excellent nutritional quality and high economic and ecological value, common buckwheat is becoming increasingly important throughout the world. The availability of a high-quality reference genome sequence and population genomic data will accelerate the breeding of common buckwheat, but the high heterozygosity due to the outcrossing nature has greatly hindered the genome assembly. Here we report the assembly of a chromosome-scale high-quality reference genome of F. esculentum var. homotropicum, a homozygous self-pollinating variant of common buckwheat. Comparative genomics revealed that two cultivated buckwheat species, common buckwheat (F. esculentum) and Tartary buckwheat (F. tataricum), underwent metabolomic divergence and ecotype differentiation. The expansion of several gene families in common buckwheat, including FhFAR genes, is associated with its wider distribution than Tartary buckwheat. Copy number variation of genes involved in the metabolism of flavonoids is associated with the difference of rutin content between common and Tartary buckwheat. Furthermore, we present a comprehensive atlas of genomic variation based on whole-genome resequencing of 572 accessions of common buckwheat. Population and evolutionary genomics reveal genetic variation associated with environmental adaptability and floral development between Chinese and non-Chinese cultivated groups. Genome-wide association analyses of multi-year agronomic traits with the content of flavonoids revealed that Fh05G014970 is a potential major regulator of flowering period, a key agronomic trait controlling the yield of outcrossing crops, and that Fh06G015130 is a crucial gene underlying flavor-associated flavonoids. Intriguingly, we found that the gene translocation and sequence variation of FhS-ELF3 contribute to the homomorphic self-compatibility of common buckwheat. Collectively, our results elucidate the genetic basis of speciation, ecological adaptation, fertility, and unique flavor of common buckwheat, and provide new resources for future genomics-assisted breeding of this economically important crop.


Asunto(s)
Productos Biológicos , Fagopyrum , Fagopyrum/genética , Metagenómica , Variaciones en el Número de Copia de ADN , Estudio de Asociación del Genoma Completo , Fitomejoramiento , Fertilidad
3.
Molecules ; 27(20)2022 Oct 20.
Artículo en Inglés | MEDLINE | ID: mdl-36296694

RESUMEN

In Tartary buckwheat (Fagopyrum tataricum), the edible parts are mainly grain and sprouts. Tartary buckwheat contains protecting substances, which make it possible for plants to survive on high altitudes and under strong natural ultraviolet radiation. The diversity and high content of phenolic substances are important for Tartary buckwheat to grow and reproduce under unfriendly environmental effects, diseases, and grazing. These substances are mainly flavonoids (rutin, quercetin, quercitrin, vitexin, catechin, epicatechin and epicatechin gallate), phenolic acids, fagopyrins, and emodin. Synthesis of protecting substances depends on genetic layout and on the environmental conditions, mainly UV radiation and temperature. Flavonoids and their glycosides are among Tartary buckwheat plants bioactive metabolites. Flavonoids are compounds of special interest due to their antioxidant properties and potential in preventing tiredness, diabetes mellitus, oxidative stress, and neurodegenerative disorders such as Parkinson's disease. During the processing and production of food items, Tartary buckwheat metabolites are subjected to molecular transformations. The main Tartary buckwheat traditional food products are bread, groats, and sprouts.


Asunto(s)
Catequina , Emodina , Fagopyrum , Fagopyrum/química , Quercetina/química , Catequina/metabolismo , Antioxidantes/farmacología , Antioxidantes/metabolismo , Rayos Ultravioleta , Emodina/metabolismo , Rutina/química , Flavonoides/química , Glicósidos/metabolismo
4.
Molecules ; 27(17)2022 Aug 30.
Artículo en Inglés | MEDLINE | ID: mdl-36080352

RESUMEN

Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) and common buckwheat (Fagopyrum esculentum Moench) are adapted to growing in harsh conditions of high altitudes. Ultraviolet radiation at high altitudes strongly impacts plant growth and development. Under the influence of ultraviolet radiation, protecting substances are synthesized in plants. The synthesis of UV-B defense metabolites is genetically conditioned, and their quantity depends on the intensity of the ultraviolet radiation to which the plants and plant parts are exposed. These substances include flavonoids, and especially rutin. Other substances with aromatic rings of six carbon atoms have a similar function, including fagopyrin, the metabolite specific for buckwheat. Defensive substances are formed in the leaves and flowers of common and Tartary buckwheat, up to about the same concentration in both species. In comparison, the concentration of rutin in the grain of Tartary buckwheat is much higher than in common buckwheat. Flavonoids also have other functions in plants so that they can protect them from pests and diseases. After crushing the grains, rutin is exposed to contact with the molecules of rutin-degrading enzymes. In an environment with the necessary humidity, rutin is turned into bitter quercetin under the action of rutin-degrading enzymes. This bitterness has a deterrent effect against pests. Moreover, flavonoids have important functions in human nutrition to prevent several chronic diseases, including obesity, cardiovascular diseases, gallstone formation, and hypertension.


Asunto(s)
Fagopyrum , Fagopyrum/metabolismo , Flavonoides/metabolismo , Humanos , Quercetina , Rutina/metabolismo , Rutina/farmacología , Rayos Ultravioleta/efectos adversos
5.
Artículo en Inglés | MEDLINE | ID: mdl-36016679

RESUMEN

Tartary buckwheat flavonoids (TBFs) exhibit diverse biological activities, with antioxidant, antidiabetes, anti-inflammatory, and cholesterol-lowering properties. In this study, we investigated the role of TBFs in attenuating glucose and lipid disturbances in diabetic mice and hence preventing the occurrence of diabetes-related colon lesions in mice by regulating the gut microbiota. The results showed that TBFs (1) reversed blood glucose levels and body weight changes; (2) improved levels of serum total cholesterol (TC), triglycerides (TGs), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and fasting insulin; and (3) significantly reduced diabetes-related colon lesions in diabetic mice. In addition, TBFs also affected the diabetes-related imbalance of the gut microbiota and enriched beneficial microbiota, including Akkermansia and Prevotella. The TBF also selectively increased short-chain fatty acid-producing bacteria, including Roseburia and Odoribacter, and decreased the abundance of the diabetes-related gut microbiota, including Escherichia, Mucispirillum, and Bilophila. The correlation analysis indicated that TBFs improved metabolic parameters related to key communities of the gut microbiota. Our data suggested that TBFs alleviated glucose and lipid disturbances and improved colon lesions in diabetic mice, possibly by regulating the community composition of the gut microbiota. This regulation of the gut microbiota composition may explain the observed effects of TBFs to alleviate diabetes-related symptoms.

6.
Int J Mol Sci ; 23(7)2022 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-35409281

RESUMEN

Tartary buckwheat (Fagopyrum tataricum Gaertn.) is grown in eastern and central Asia (the Himalayan regions of China, Nepal, Bhutan and India) and in central and eastern Europe (Luxemburg, Germany, Slovenia and Bosnia and Herzegovina). It is known for its high concentration of rutin and other phenolic metabolites. Besides the grain, the other aboveground parts of Tartary buckwheat contain rutin as well. After the mixing of the milled buckwheat products with water, the flavonoid quercetin is obtained in the flour-water mixture, a result of rutin degradation by rutinosidase. Heating by hot water or steam inactivates the rutin-degrading enzymes in buckwheat flour and dough. The low buckwheat protein digestibility is due to the high content of phenolic substances. Phenolic compounds have low absorption after food intake, so, after ingestion, they remain for some time in the gastrointestinal tract. They can act in an inhibitory manner on enzymes, degrading proteins and other food constituents. In common and Tartary buckwheat, the rutin and quercetin complexation with protein and starch molecules has an impact on the in vitro digestibility and the appearance of resistant starch and slowly digestible proteins. Slowly digestible starch and proteins are important for the functional and health-promoting properties of buckwheat products.


Asunto(s)
Fagopyrum , Grano Comestible , Fagopyrum/metabolismo , Fenoles/metabolismo , Quercetina/metabolismo , Rutina/metabolismo , Semillas , Almidón/metabolismo , Agua/metabolismo
7.
Food Chem ; 283: 28-31, 2019 Jun 15.
Artículo en Inglés | MEDLINE | ID: mdl-30722872

RESUMEN

The aim was to determine conditions under which rutin can be retained during production of Tartary buckwheat (Fagopyrum tataricum) dough. Tartary buckwheat flour was hydrothermally treated by mixing with water at 25, 40, 60, 80 and 95 °C, with unprocessed Tartary buckwheat flour as control. With hydrothermal treatments at 25, 40 and 60 °C, most of the rutin was transformed to quercetin. However, for hydrothermal treatments at 80 and 95 °C, rutin was retained due to denaturation of the rutin-degrading enzymes during hydrothermal treatment. This is the first report to describe a temperature threshold for denaturation of rutin-degrading enzymes in any buckwheat material. Tartary buckwheat dough produced at 95 °C contained 12 mg rutin/g dry matter. Based on these characteristics, dough from hydrothermally treated Tartary buckwheat is a promising, rutin-rich functional food material.


Asunto(s)
Fagopyrum/química , Harina , Quercetina/química , Rutina/química , Harina/análisis , Alimentos Funcionales , Quercetina/aislamiento & purificación , Rutina/aislamiento & purificación , Temperatura
8.
J Photochem Photobiol B ; 180: 51-55, 2018 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-29413701

RESUMEN

Plants of the genus Fagopyrum contain high levels of crystalline calcium oxalate (CaOx) deposits, or druses, that can affect the leaf optical properties. As selenium has been shown to modify the uptake and accumulation of metabolically important elements such as calcium, we hypothesised that the numbers of druses can be altered by selenium treatment, and this would affect the leaf optical properties. Tartary buckwheat (Fagopyrum tataricum Gaertn.) was grown outdoors in an experimental field. At the beginning of flowering, plants were foliarly sprayed with sodium selenate solution at 10 mg selenium L-1 or only with water. Plant morphological, biochemical, physiological and optical properties were examined, along with leaf elemental composition and content. Se spraying did not affect leaf biochemical and functional properties. However, it increased leaf thickness and the contents of Se in the leaves, and decreased the density of calcium oxalate druses in the leaves. Except Se content, Se spraying did not affect contents of other elements in leaves, including total calcium per dry mass of leaf tissue. Redundancy analysis showed that of all parameters tested, only the calcium oxalate druses parameters were significant in explaining the variability of the leaf reflectance and transmittance spectra. The density of CaOx druses positively correlated with the reflectance in the blue, green, yellow and UV-B regions of the spectrum, while the area of CaOx druses per mm2 of leaf transection area positively correlated with the transmittance in the green and yellow regions of the spectrum.


Asunto(s)
Oxalato de Calcio/química , Oxalato de Calcio/farmacología , Fagopyrum/efectos de los fármacos , Hojas de la Planta/efectos de los fármacos , Selenio/farmacología , Clorofila/química , Clorofila A , Fagopyrum/química , Fagopyrum/efectos de la radiación , Hojas de la Planta/química , Hojas de la Planta/efectos de la radiación , Selenio/química , Espectrometría por Rayos X , Rayos Ultravioleta
9.
Plant Foods Hum Nutr ; 73(1): 82-88, 2018 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-29435700

RESUMEN

In this report, we presented the profile of polyphenolic substances in flowers, leaves, stalk and roots of Fagopyrum tataricum estimated by using RP-UHPLC-ESI-MS equipment (reversed-phase ultra-high-performance liquid chromatography electrospray ionisation mass spectrometry). The neutral detergent fibre, acid detergent fibre, acid detergent lignin, cellulose and hemicellulose were also determined. Flowers, leaves, stalk and roots showed varying levels of dietary fibre and polyphenols. The highest content of neutral and acid detergent fibre were found in the roots (63.92 and 45.45% d.m., respectively) while the most rich in phenolic compounds were flowers (4.8 mg/1 g d.m.). Root and stalk contained the highest level of cellulose, 38.70 and 25.57% d.m., respectively. Among the investigated polyphenolic substances such as: 2,6-dihydroxybenzoic acid, 3,4-dihydroxybenzoic acid, 3,5-dihydroxybenzoic acid, 4-hydrobenzoic acid, caffeic acid, catechin, chlorogenic acid, fagopyrin, ferulic acid, myricetin, gallic acid, isovanilic acid, isovitexin, kaempferol, luteolin, p-coumaric acid, procyanidin B2, quercetin, quercetin 3-D galactoside, rutin, syringic acid and vitexin, we observed that the contents of rutin and chlorogenic acid were the highest. We found some correlation between dietary fibre fractions and individual phenolic substances. The levels of acid detergent fibre (ADF), cellulose and hemicellulose were negatively correlated with isovitexin, kaempferol, vitexin, fagopyrin, caffeic acid and procyanidin B2 content. In this investigation, two solvents (water and methanol) were estimated regarding their extraction efficiency of phenolic compounds. Taking these results into consideration, we recommend using methanol as the extractor to isolate chlorogenic acid, fagopyrin, kaempferol, procyanidin B2, quercetin, quercetin 3-D-galactoside, rutin, vitexin, and water for other investigated polyphenolic substances obtained from Fagopyrum tataricum.


Asunto(s)
Fibras de la Dieta/análisis , Fagopyrum/química , Polifenoles/análisis , Fraccionamiento Químico/métodos , Cromatografía Líquida de Alta Presión/métodos , Flores/química , Metanol/química , Hojas de la Planta/química , Raíces de Plantas/química , Espectrometría de Masa por Ionización de Electrospray , Agua/química
10.
New Phytol ; 216(3): 814-828, 2017 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-28722263

RESUMEN

Little is known about the molecular mechanism of the R2R3-MYB transcriptional repressors involved in plant phenylpropanoid metabolism. Here, we describe one R2R3-type MYB repressor, FtMYB11 from Fagopyrum tataricum. It contains the SID-like motif GGDFNFDL and it is regulated by both the importin protein 'Sensitive to ABA and Drought 2' (SAD2) and the jasmonates signalling cascade repressor JAZ protein. Yeast two hybrid and bimolecular fluorescence complementation assays demonstrated that FtMYB11 interacts with SAD2 and FtJAZ1. Protoplast transactivation assays demonstrated that FtMYB11 acts synergistically with FtSAD2 or FtJAZ1 and directly represses its target genes via the MYB-core element AATAGTT. Changing the Asp122 residue to Asn in the SID-like motif results in cytoplasmic localization of FtMYB11 because of loss of interaction with SAD2, while changing the Asp126 residue to Asn results in the loss of interaction with FtJAZ1. Overexpression of FtMYB11or FtMYB11D126N in F. tataricum hairy roots resulted in reduced accumulation of rutin, while overexpression of FtMYB11D122N in hairy roots did not lead to such a change. The results indicate that FtMYB11 acts as a regulator via interacting with FtSAD2 or FtJAZ1 to repress phenylpropanoid biosynthesis, and this repression depends on two conserved Asp residues of its SID-like motif.


Asunto(s)
Fagopyrum/metabolismo , Regulación de la Expresión Génica de las Plantas , Proteínas de Plantas/metabolismo , Secuencias de Aminoácidos , Arabidopsis/genética , Ácido Aspártico/genética , Ácido Aspártico/metabolismo , Citoplasma/metabolismo , Fagopyrum/genética , Prueba de Complementación Genética , Mutación , Fenilpropionatos/metabolismo , Proteínas de Plantas/genética , Raíces de Plantas/genética , Raíces de Plantas/metabolismo , Plantas Modificadas Genéticamente , Proteínas Represoras/genética , Proteínas Represoras/metabolismo , Rutina/biosíntesis , Rutina/genética , Factores de Transcripción/genética , Factores de Transcripción/metabolismo
11.
Plant Physiol Biochem ; 108: 32-36, 2016 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-27404132

RESUMEN

Tartary buckwheat (Fagopyrum tataricum Gaertn.) and common buckwheat (Fagopyrum esculentum Moench.) plants grown in the field were treated foliarly with 126 µM solutions of selenate and/or sulphate in order to study the effect of sulphur (S) on selenium (Se) concentration in plants. In both species, the concentration of Se in all plant parts was similar in control and S treated plants. In Tartary buckwheat the concentration of Se was higher in S and Se treated plants than in plants treated with Se alone. S was shown to enhance Se accumulation in Tartary buckwheat. It was also shown that it is possible to produce grain and herb of Tartary and common buckwheat containing appropriate amounts of Se for food without affecting the yield of the plants.


Asunto(s)
Fagopyrum/efectos de los fármacos , Fagopyrum/metabolismo , Selenio/farmacocinética , Azufre/farmacología , Fagopyrum/crecimiento & desarrollo , Hojas de la Planta/efectos de los fármacos , Ácido Selénico/farmacología , Sulfatos/farmacología
12.
Food Chem ; 199: 782-90, 2016 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-26776035

RESUMEN

Tartary buckwheat is a gluten-free crop with great potential as a wheat substitute. Iron (Fe) is an important mineral element in staple foods which is required in sufficient bioaccessible quantities. The aim of the study was to investigate how processing of grains into groats (hydrothermal processing to remove the husk) and sprouts (7-day-old seedlings) affected Fe speciation (Fe(2+) or Fe(3+)), Fe ligand composition and Fe bioaccessibility to human Caco-2 cells. Groats contained the least Fe (23.8 ± 1.65 mg kg(-1)) and the lowest amounts of Fe(2+) (8%). Grains and sprouts had comparable Fe concentrations (78.2 ± 2.65 and 68.9 ± 2.73 mg kg(-1)) and similar proportions of Fe(2+) (15% and 18%). The main ligands for Fe in Tartary buckwheat material were phytate and citrate. Phytate was less abundant in sprouts, which did not correlate with greater Fe bioaccessibility. Iron bioaccessibility was 4.5-fold greater for grains than groats, suggesting that Fe is more bioaccessible in the husk than in the rest of the grain.


Asunto(s)
Células CACO-2/química , Fagopyrum/química , Hierro/metabolismo , Grano Comestible , Germinación , Humanos , Plantones
13.
Food Chem ; 190: 552-558, 2016 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-26213009

RESUMEN

A starch rich fraction is a side product in Tartary buckwheat processing. This study investigated the fractions that are of technological and nutritional interest. Tartary buckwheat starch granules had a diameter of 3-14 µm, and presented a typical type "A" X-ray diffraction pattern. They contained nearly 39.0% amylose. The solubility of Tartary buckwheat starch was much lower at 70-90 °C (ranging within 9.9-10.4% at 90 °C) than that in maize (up to 49.3%) and potato (up to 85.0%) starch. The starch of one variety of Tartary buckwheat had significantly lower solubility at 70 °C and 80 °C than that of common buckwheat. The starch peak viscosity and breakdown were higher and pasting time was shorter in Tartary buckwheat than in that of the starch of common buckwheat. Tartary buckwheat starch had unique pasting and physicochemical properties, and is thereby capable of being exploited as a suitable raw material of retrograded starch in food processing.


Asunto(s)
Fagopyrum/química , Alimentos Funcionales/análisis , Almidón/química , Fenómenos Químicos
14.
Food Chem ; 165: 232-40, 2014 Dec 15.
Artículo en Inglés | MEDLINE | ID: mdl-25038671

RESUMEN

In this study, chia seed flour, which is rich in omega-3 alpha-linolenic acid, and common and tartary buckwheat flour, which has a high antioxidant activity, were integrated into different types of bread with the aim of improving their nutritional value and healthy features. Our results indicate that bread made with chia and tartary buckwheat flour was more acceptable in many nutritional aspects compared to the control (common wheat bread); it contained a higher amount of protein (20%), insoluble dietary fibres (74%), ash (51%), and alpha-linolenic acid (67.4%). Moreover, this bread possessed lower energy (14%) and carbohydrate contents (24%) compared to the control. Tartary buckwheat also improved the total antioxidant capacity of the bread (about 75%) and provided a considerable amount of flavonoids, which are healthy non-nutritional compounds. Overall, chia and tartary buckwheat represent excellent raw materials for the formulation of gluten-free bread with high nutritional value.


Asunto(s)
Pan/análisis , Fagopyrum/química , Ácidos Grasos Omega-3/análisis , Flavonoides/análisis , Harina/análisis , Glútenes/química , Fibras de la Dieta/análisis , Valor Nutritivo , Semillas/química
15.
J Med Food ; 16(10): 944-52, 2013 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-24083484

RESUMEN

The aim of our work was to determine and to compare the possible antigenotoxic effect of methanolic extracts of common buckwheat (CB) and Tartary buckwheat (TB) flour, containing naturally present rutin (R), and quercetin (Q), and of R and Q in chemical form, against tert-butyl hydroperoxide (t-BOOH) induced DNA damage in human hepatoma cell line (HepG2). R and Q content of CB and TB flour extracts was determined by reversed phase-high performance liquid chromatography and antigenotoxic effect of flour extracts, R and Q was evaluated using the comet assay. R (100 µM) and Q (50 µM) decreased the extent of t-BOOH induced DNA damage for 51% and 67%, respectively. CB and TB flour extracts showed high antioxidant capacity and prominent genoprotective ability. CB extract containing up to 0.1 µM R decreased t-BOOH induced DNA damage for 34%, and TB extract containing up to 12.64 µM R, and 2.86 µM Q for 40%. The obtained results show high antigenotoxic activity of buckwheat and furthermore, they suggest that complex nutrient and flavonoid rich food products are more efficient in their health promoting effects compared to a single active substance.


Asunto(s)
Antimutagênicos/farmacología , Antioxidantes/farmacología , Daño del ADN/efectos de los fármacos , Fagopyrum/química , Harina/análisis , Extractos Vegetales/farmacología , Quercetina/farmacología , Rutina/farmacología , Células Hep G2 , Humanos
16.
Plant Physiol Biochem ; 63: 196-9, 2013 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-23274247

RESUMEN

To investigate the impact of Se on Tartary buckwheat (Fagopyrum tataricum Gaertn.) plants, the plant foliage was sprayed with 10 mg Se(VI) L(-1) at the beginning of flowering. The Se was effectively assimilated by the plants and taken into the seeds, where its concentration was more than double that in untreated plants. The seeds were collected and sown to obtain the progeny of these Se-treated plants. To assess the physiological characteristics of control plants and these Se-treated progeny plants, the estimated respiratory potential via electron transport system (ETS) activity and the photochemical efficiency of photosystem II were measured. Three weeks after germination, the Se-treated progeny plants showed higher ETS activity compared to the controls. Through weeks 4 and 5, this high ETS activity approximately halved, and the difference in ETS activity seen at 3 weeks was lost. On the other hand, at week 4, the potential photochemical efficiency was higher in the Se-treated progeny plants than the controls. In adult plants, the leaves dry mass was significantly greater in the Se-treated progeny plants than the controls. This study demonstrates an impact of Se in Tartary buckwheat on the progeny plants of Se sprayed plants, as shown previously in pea plants.


Asunto(s)
Fagopyrum/efectos de los fármacos , Fagopyrum/metabolismo , Mitocondrias/metabolismo , Selenio/toxicidad , Complejo de Proteína del Fotosistema II/efectos de los fármacos , Complejo de Proteína del Fotosistema II/metabolismo
17.
Acta Pharm ; 63(4): 553-60, 2013 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-24451079

RESUMEN

Dried buckwheat herb is used in medicinal products whereas fresh green plant parts, especially sprouts, are consumed as a vegetable. The herb contains fagopyrins, which cause sensitivity to light after ingestion. The aim of this study was to investigate the impact of different growing conditions and the development phase on the content of fagopyrin and phenolic compounds in buckwheat sprouts. Total flavonoid and total phenol contents, fagopyrin content and antioxidant activity were determined spectrophotometrically. Fagopyrin and flavonoids were located almost exclusively in cotyledons. Based on a comparison to hypericin toxicity, the recommendable intake of buckwheat sprouts was estimated to be less than 40 g per day.


Asunto(s)
Fagopyrum/química , Flavonoides/química , Polifenoles/química , Quinonas/química , Antioxidantes/química , Antioxidantes/aislamiento & purificación , Antioxidantes/farmacología , Dermatitis Fototóxica/etiología , Fagopyrum/efectos adversos , Fagopyrum/crecimiento & desarrollo , Flavonoides/aislamiento & purificación , Flavonoides/farmacología , Polifenoles/aislamiento & purificación , Quinonas/aislamiento & purificación , Ingesta Diaria Recomendada , Espectrofotometría
18.
Tohoku J Exp Med ; 225(2): 123-30, 2011 10.
Artículo en Inglés | MEDLINE | ID: mdl-21931228

RESUMEN

Buckwheat food is a good source of antioxidants, e.g. rutin, and other beneficial substances. Here we investigated the effects of the intake of common buckwheat (low rutin content) and tartary buckwheat cookies (high rutin content) on selected clinical markers. A double blind crossover study was performed among female day-care centre staffs (N = 62) from five day-care centres. Participants were randomly divided into two groups. The first group initially consumed four common buckwheat cookies per day (16.5 mg rutin equivalents/day) for two weeks, while the second group consumed four tartary buckwheat cookies per day (359.7 mg rutin equivalents/day). Then the groups switched their type of cookies and consumed them for another two weeks. We monitored selected clinical markers related to cardiovascular disease and lower airway inflammation, lung function, and subjective breathing difficulties in the staffs. Intake of tartary buckwheat cookies reduced the serum level of myeloperoxidase (MPO) by a factor 0.84 (p = 0.02). When grouping the two types of buckwheat cookies together, there was a reduction of total serum cholesterol (p < 0.001) and HDL-cholesterol (p < 0.001) during the study period, with improved lung vital capacity (p < 0.001). The degree of reduction in total and HDL cholesterol levels was similar in staffs with low and high body mass index (cut off 25). In conclusion, intake of tartary buckwheat cookies with high level of the antioxidant rutin may reduce levels of MPO, an indicator of inflammation. Moreover, intake of both types of buckwheat cookies may lower cholesterol levels.


Asunto(s)
Antioxidantes/farmacocinética , Colesterol/sangre , Fagopyrum/química , Peroxidasa/sangre , Extractos Vegetales/farmacocinética , Antioxidantes/administración & dosificación , Centros de Día , Dieta , Fibras de la Dieta/administración & dosificación , Método Doble Ciego , Ingestión de Alimentos , Femenino , Flavonoides/análisis , Alimentos , Humanos , Cuerpo Médico , Persona de Mediana Edad , Extractos Vegetales/administración & dosificación , Extractos Vegetales/química
19.
J Exp Bot ; 62(11): 3929-39, 2011 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-21447756

RESUMEN

Mature developed seeds are physiologically and biochemically committed to store nutrients, principally as starch, protein, oils, and minerals. The composition and distribution of elements inside the aleurone cell layer reflect their biogenesis, structural characteristics, and physiological functions. It is therefore of primary importance to understand the mechanisms underlying metal ion accumulation, distribution, storage, and bioavailability in aleurone subcellular organelles for seed fortification purposes. Synchrotron radiation soft X-ray full-field imaging mode (FFIM) and low-energy X-ray fluorescence (LEXRF) spectromicroscopy were applied to characterize major structural features and the subcellular distribution of physiologically important elements (Zn, Fe, Na, Mg, Al, Si, and P). These direct imaging methods reveal the accumulation patterns between the apoplast and symplast, and highlight the importance of globoids with phytic acid mineral salts and walls as preferential storage structures. C, N, and O chemical topographies are directly linked to the structural backbone of plant substructures. Zn, Fe, Na, Mg, Al, and P were linked to globoid structures within protein storage vacuoles with variable levels of co-localization. Si distribution was atypical, being contained in the aleurone apoplast and symplast, supporting a physiological role for Si in addition to its structural function. These results reveal that the immobilization of metals within the observed endomembrane structures presents a structural and functional barrier and affects bioavailability. The combination of high spatial and chemical X-ray microscopy techniques highlights how in situ analysis can yield new insights into the complexity of the wheat aleurone layer, whose precise biochemical composition, morphology, and structural characteristics are still not unequivocally resolved.


Asunto(s)
Semillas/ultraestructura , Triticum/ultraestructura , Vacuolas/ultraestructura , Metales/química , Fósforo/química , Ácido Fítico/química , Semillas/química , Espectrometría por Rayos X , Sincrotrones , Vacuolas/química , Rayos X
20.
J Agric Food Chem ; 59(4): 1275-80, 2011 Feb 23.
Artículo en Inglés | MEDLINE | ID: mdl-21226516

RESUMEN

The elemental composition of specific fractions of cereal and pseudocereal grains can be roughly estimated after milling. Alternatively, the elemental localization of cross-sectioned grains can be quantitatively analyzed by microproton induced X-ray emission (micro-PIXE), taking advantage of high elemental sensitivity and low lateral resolution. We present a micro-PIXE study on buckwheat (Fagopyrum esculentum) grain, with a detailed description of the elemental distributions. Elements such as Mg, P, S, K, Fe, Ni, Cu, and Zn were preferentially localized in the cotyledons and embryonic axis; however, significant amounts of K and Fe were also found in the pericarp. The aleurone layer covering the cotyledons was especially enriched in S and P, while testa, a thin layer above the aleurone did not show any significant element enrichments. The highest concentrations of Al, Si, Cl, Ca, and Ti were found in the pericarp. A detailed element localization study of pericarp layers revealed that the inner layer was enriched in K, Mn, Ca, and Fe, while the outer layer showed enrichments in Na, Mg, P, S, and Al. On the basis of the data obtained, milling techniques can be adapted to obtain milling fractions with targeted nutritional values.


Asunto(s)
Fagopyrum/química , Semillas/química , Espectrometría por Rayos X/métodos , Oligoelementos/análisis , Cotiledón/química , Endospermo/química , Manipulación de Alimentos/métodos , Semillas/anatomía & histología
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