RESUMEN
BACKGROUND: The main objective of the present study is to quantify doxycycline (DOX) release from ß-tricalcium phosphate (ß-TCP) after EDTA root surface treatment. METHODS: Thirty systemically healthy patients with ≥1 paired contralateral interproximal intrabony defect ≥4 mm deep along with an interproximal probing depth ≥6 mm and clinical attachment level ≥4 mm were randomized into two groups. Group 1 (G1) consisted of sites treated with open flap debridement followed by placement of DOX blended with ß-TCP (DOX-ß-TCP), whereas group 2 (G2) sites were treated with flap surgery followed by the placement of DOX blended with ß-TCP after EDTA etching of the exposed root surfaces (DOX-ß-TCP + EDTA). Samples of gingival crevicular fluid (GCF) were obtained 1, 3, 7, 14, and 21 days after surgery. Quantitative measurements of DOX were taken with high-performance liquid chromatography. Clinical evaluation and follow-up for 6 months were performed. RESULTS: At 21 days, the DOX-ß-TCP + EDTA-treated group showed a 194.7 µg/mL value. The DOX-ß-TCP + EDTA-treated group retained more DOX during the periods of 3, 7, 10, 14, and 21 days than the DOX-ß-TCP-treated group. Six months after therapy, DOX-ß-TCP + EDTA-treated sites showed more significant clinical improvements compared to DOX-ß-TCP-treated sites (P ≤ 0.05). CONCLUSIONS: EDTA root surface etching enhances DOX availability in the GCF following its release from ß-TCP as a drug carrier.
Asunto(s)
Grabado Ácido Dental/métodos , Materiales Biocompatibles/química , Fosfatos de Calcio/química , Periodontitis Crónica/cirugía , Doxiciclina/farmacocinética , Ácido Edético/uso terapéutico , Inhibidores de la Metaloproteinasa de la Matriz/farmacocinética , Raíz del Diente/efectos de los fármacos , Adulto , Pérdida de Hueso Alveolar/cirugía , Cromatografía Líquida de Alta Presión , Desbridamiento , Doxiciclina/administración & dosificación , Doxiciclina/análisis , Portadores de Fármacos , Femenino , Estudios de Seguimiento , Líquido del Surco Gingival/química , Humanos , Masculino , Inhibidores de la Metaloproteinasa de la Matriz/administración & dosificación , Inhibidores de la Metaloproteinasa de la Matriz/análisis , Persona de Mediana Edad , Pérdida de la Inserción Periodontal/cirugía , Índice Periodontal , Bolsa Periodontal/cirugía , Método Simple Ciego , Colgajos Quirúrgicos/cirugía , Resultado del TratamientoRESUMEN
BACKGROUND: The release profile of 25% doxycycline (DOX) gel loaded on a biodegradable collagen membrane (COL) after 24% EDTA root surface etching was evaluated. METHODS: Thirty systemically healthy patients, each with at least one pair of contralateral interproximal intrabony defects ≥4 mm deep, along with an interproximal probing depth ≥6 mm and clinical attachment loss ≥4 mm, were randomized into two groups. Group 1 consisted of sites treated with open-flap debridement followed by placement of DOX gel-loaded COL (DOX-COL), whereas group 2 sites were treated with flap surgery followed by the placement of DOX-COL after EDTA etching of the exposed root surfaces (DOX-COL + EDTA). Samples of gingival crevicular fluid were obtained 1, 3, 7, 14, and 21 days after surgery. Separation was performed, and quantitative measurements of DOX were taken with a high-performance liquid chromatography. Clinical evaluation and follow-up for 6 months were performed. RESULTS: At 21 days, DOX-COL + EDTA group showed 5.3 µg/mL value. However, no DOX was detected in samples of the DOX-COL group. DOX-COL + EDTA-treated group retained more DOX during the periods of 3, 7, 10, and 14 days than did the DOX-COL group. CONCLUSION: EDTA root surface etching could enhance DOX availability in the gingival crevicular fluid after its release from the collagen membrane.
Asunto(s)
Implantes Absorbibles , Grabado Ácido Dental/métodos , Antibacterianos/administración & dosificación , Quelantes/uso terapéutico , Periodontitis Crónica/terapia , Doxiciclina/administración & dosificación , Ácido Edético/uso terapéutico , Membranas Artificiales , Raíz del Diente/efectos de los fármacos , Adulto , Pérdida de Hueso Alveolar/terapia , Antibacterianos/análisis , Cromatografía Líquida de Alta Presión , Colágeno/química , Preparaciones de Acción Retardada , Raspado Dental/métodos , Difusión , Doxiciclina/análisis , Femenino , Estudios de Seguimiento , Geles , Líquido del Surco Gingival/química , Humanos , Masculino , Persona de Mediana Edad , Bolsa Periodontal/terapia , Aplanamiento de la Raíz/métodos , Colgajos QuirúrgicosRESUMEN
BACKGROUND: The main objective of the present study was to quantify chlorhexidine (CHX) release after the use of CHX-EDTA root surface treatment as a local-delivery antimicrobial vehicle. METHODS: Twenty non-smoking patients clinically diagnosed as having moderate-to-severe chronic periodontitis were selected to participate in this study. After cause-related therapy, one site in every patient received defect overfill with CHX gel 2% (20 sites). In addition, twenty contralateral sites received defect fill of CHX gel after 3 minutes of 24% EDTA gel root surface etching (20 sites). Gingival crevicular fluid samples were collected at 1, 3, 7, and 14 days post-therapy. RESULTS: The CHX-EDTA group showed statistically significantly higher levels of CHX than those of the control group at 1, 3, and 7 days. At 14 days, the CHX-EDTA group showed 0.8 mg/mL values. CONCLUSION: The use of CHX-EDTA root surface treatment as a local-delivery antimicrobial improves CHX substantivity.