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Int J Cancer ; 112(6): 1029-35, 2004 Dec 20.
Artículo en Inglés | MEDLINE | ID: mdl-15386348

RESUMEN

We applied serological analysis of recombinant cDNA expression libraries (SEREX) to cases of esophageal squamous cell carcinoma (SCC) to identify tumor antigens. One of the clones identified was TROP2, which is known as calcium signal transducer. To evaluate the clinical significance of serum anti-TROP2 antibodies (s-TROP2-Abs) in patients with esophageal SCC, the presence of s-TROP2-Abs was analyzed by Western blotting using bacterially expressed TROP2 protein. We found that 23 of 75 (31%) patients were positive for s-TROP2-Abs. Positivity in terms of s-TROP2-Abs showed a significant association with tumor size but not with other clinicopathological features. The protein expression levels of TROP2 were much higher in esophageal SCC cell lines as compared to those in normal esophageal mucosa and its immortalized cells although the mRNA expression levels were not necessarily elevated in malignant cell lines and tissues. Immunohistochemical studies showed that the expression of TROP2 protein in esophageal SCC specimens was noticeably higher than that found in mild hyperplasia of esophageal mucosae. Thus, s-TROP2-Abs seemed useful in the diagnosis of SCC and may be a candidate for serum tumor markers.


Asunto(s)
Antígenos de Neoplasias/análisis , Biomarcadores de Tumor/análisis , Carcinoma de Células Escamosas/química , Carcinoma de Células Escamosas/diagnóstico , Moléculas de Adhesión Celular/análisis , Neoplasias Esofágicas/química , Neoplasias Esofágicas/diagnóstico , Anciano , Antígenos de Neoplasias/inmunología , Biomarcadores de Tumor/inmunología , Western Blotting , Moléculas de Adhesión Celular/inmunología , Línea Celular Tumoral , Clonación Molecular/métodos , ADN Complementario/análisis , ADN de Neoplasias/análisis , Molécula de Adhesión Celular Epitelial , Femenino , Regulación Neoplásica de la Expresión Génica , Humanos , Inmunohistoquímica , Masculino , Persona de Mediana Edad , Proteínas Recombinantes/análisis
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