Ultrasound-assisted adsorption/desorption of jujube peel flavonoids using macroporous resins.

The work explored the process of ultrasound-assisted adsorption/desorption to efficiently purify jujube peel flavonoids (JPFs) using macroporous resins (MRs). The impact of ultrasound power and temperature on the adsorption/desorption features of JPFs on MRs were studied. The maximum adsorption (80.21 ± 2.11 mg/g) /desorption (76.22 ± 1.68 mg/g) capacity of total flavonoids content were obtained. The pseudo-second-order kinetic and Freundlich isotherm models better described the whole process of ultrasound-assisted adsorption. The adsorption process was spontaneous, physical, and dominated by multilinear intraparticle diffusion. Ultrasound mainly enhanced the adsorption capacity by strengthening the formation of hydrogen bonds and increasing the surface roughness of MRs. Besides, the principal individual flavonoid ((+)-Catechin, (-)-Epicatechin, Rutin, Quercetin-3-O-robinobioside) content of JPFs in ultrasound treatment was 2-3 times that of shaking treatment, and biological activities were significantly increased. Overall, as a low-cost green technology, ultrasound can improve the properties of MRs and better purify JPFs.

Age-dependent alteration in metabolism of vitamin B6 , neurotransmitters, and amino acids after 4'-O-methylpyridoxine administration in rats.

4'-O-methylpyridoxine (MPN), a recognized antivitamin B6 compound, is a potentially poisonous substance found in Ginkgo biloba L. In this work, the effects of MPN on the metabolism of vitamin B6 , neurotransmitters, and amino acids were compared in the plasma and brain of young and adult rats under various administration times. Results showed that the contents of MPN residues in the plasma and brain of young rats were 12.72 and 14.76 µM higher than adult rats, respectively. Moreover, the levels of 5-hydroxytryptamine and dopamine in the brain of young rats have decreased by 13.78% and 7.19%, respectively, compared with the control group, at 2 h after MPN administration. Furthermore, the principal component analysis revealed that MPN was an important contributor to the amino acid composition in the brain of young rats. These results suggest that age may lead to different toxic effects of MPN. PRACTICAL APPLICATION: 4'-O-methylpyridoxine is primarily responsible for poisoning due to overconsumption of Ginkgo biloba seeds. This study will provide an exploratory understanding of the age-dependent toxicity of 4'-O-methylpyridoxine.

In vivo toxicity assessment of 4'-O-methylpyridoxine from Ginkgo biloba seeds: Growth, hematology, metabolism, and oxidative parameters.

4'-O-methylpyridoxine (MPN), a recognized antivitamin B6 compound, is a potentially poisonous substance found in Ginkgo biloba seeds and leaves. In this work, the body weights, histopathological changes, plasma vitamin B6 (VB6), biochemical parameters, oxidative stress responses, and amino acids of rats were investigated after intragastric administration of MPN for 15 days. Results showed that intragastric administration of 50 mg/kg BW MPN caused pathological changes in the brain and heart tissues of rats. Administration of 10 mg/kg and 30 mg/kg BW MPN can significantly increase VB6 analogs in the plasma of rats, such as pyridoxal-5'-phosphate, pyridoxal. Results of biochemical parameters indicated that MPN can damage brains and hearts by changing the enzyme activity of these organs. These results suggest that consumption of Ginkgo biloba seeds for the long term, even in a small quantity, may lead to poisoning.