RESUMEN
Two new terpenoids (1-2) and seven known compounds (3-9) were isolated from methanol extract of Callicarpa macrophylla leaves. Their structures were determined to be ent-7α,16ß,17,18-tetrahydroxykaur-15-one (1), 3ß-acetoxy-urs-12-ene-11-one-12-ol (2), ent-1ß-acetoxy-7α,14ß-dihydroxykaur-16-en-15-one (3), 3ß-acetoxy-11α,13ß-dihydroxyolean-12-one (4), ß-amyrin (5), spinasterol (6), ursolic acid (7), ß-sitosterol (8), and daucosterol (9) by analyses of their MS, NMR spectroscopic data and by comparison with those reported in the literature. Compounds 1 - 4, and 7 displayed potential cytotoxic activity towards HepG-2, LU-1, and MCF-7 human cancer cell lines with IC50 values ranging from 0.46 ± 0.21 to 18.14 ± 0.33 µM. Compound 6 showed IC50 values of 14.17 ± 0.21 and 5.72 ± 0.42 µM against Hep-G2 and MCF-7 cell lines, respectively.
Asunto(s)
Callicarpa/química , Terpenos/aislamiento & purificación , Espectroscopía de Resonancia Magnética con Carbono-13 , Línea Celular Tumoral , Humanos , Concentración 50 Inhibidora , Ácido Oleanólico/análogos & derivados , Ácido Oleanólico/química , Ácido Oleanólico/farmacología , Extractos Vegetales/análisis , Extractos Vegetales/química , Extractos Vegetales/farmacología , Hojas de la Planta/química , Espectroscopía de Protones por Resonancia Magnética , Terpenos/análisis , Terpenos/química , Terpenos/farmacología , Triterpenos/química , Triterpenos/farmacología , Ácido UrsólicoRESUMEN
One new schiartane-type triterpene, heteroclitalactone N (1), and four known compounds (2-5), seco-coccinic acid F, dihydroguaiaretic acid, schizanrin F, and kadsuralignan B were isolated from the stems of Kadsura heteroclita. Their structures were determined by the combination of spectroscopic and chemical methods, including 1D-, 2D-NMR, and CD spectra as well as by comparing with the NMR data reported in the literature. The cytotoxic activities of all isolated compounds were evaluated on three human cancer cell lines. Compound 3 exhibited moderate to weak cytotoxic activities on three human cancer cell lines, OVCAR, HT-29, and A-549, with IC50 values ranging from 16.2 to 36.4 microM.