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2.
J Dairy Sci ; 102(4): 2844-2853, 2019 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-30799108

RESUMEN

Synbiotics, a combination of prebiotics and probiotics, produce synergistic effects to promote gastrointestinal health. Herein, we investigated the synbiotic interaction between the Lactobacillus rhamnosus strain GG (LGG; a probiotic strain) and tagatose (a prebiotic) in a dextran sulfate sodium (DSS)-induced colitis murine model. Initially, body weight, food intake, and clinical features were dramatically decreased after treatment with DSS, and the addition of LGG, tagatose, or both ameliorated these effects. In our pyrosequencing analysis of fecal microbiota, DSS treatment increased the abundance of Proteobacteria and decreased that of Firmicutes. When LGG and tagatose were administered as synbiotics, the gut microbiota composition recovered from the dysbiosis caused by DSS treatment. In particular, the abundance of Bacteroides, Lactobacillus, and Akkermansia was significantly associated with probiotic, prebiotic, and synbiotic treatments. Taken together, our results suggest that LGG and tagatose as synbiotics can alleviate colitis, and synbiotics could be applied as dietary supplements in dairy foods such as yogurt and cheese.


Asunto(s)
Colitis/inducido químicamente , Colitis/terapia , Hexosas/uso terapéutico , Lacticaseibacillus rhamnosus , Simbióticos , Animales , Sulfato de Dextran/toxicidad , Heces/microbiología , Hexosas/administración & dosificación , Quelantes del Hierro/administración & dosificación , Quelantes del Hierro/farmacología , Lactobacillus , Lacticaseibacillus rhamnosus/clasificación , Ratones , Microbiota
3.
Ann Oncol ; 24(2): 489-494, 2013 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-23110809

RESUMEN

BACKGROUND: We evaluated whether complementary and alternative medicine (CAM) use influenced outcomes [survival and health-related quality of life (HRQOL)] of cancer patients whose condition had just been judged terminal. PATIENTS AND METHODS: From July 2005 to October 2006, we conducted a prospective cohort study of 481 terminally ill cancer patients at 11 university hospitals and the National Cancer Center in Korea. We assessed how the use of CAM affected HRQOL and survival. RESULTS: In a follow-up of 481 patients and 163.8 person-years, we identified 466 deceased cases. On multivariate analyses, CAM users did not have better survival compared with nonusers [adjusted hazard ratio (aHR), 0.91; 95% confidence interval (CI) 0.74-1.10]. Among mind-body interventions, prayer showed significantly worse survival (aHR, 1.56; 95% CI, 1.00-2.43). Clinically, CAM users reported significantly worse cognitive functioning (-11.6 versus -1.3; P < 0.05) and fatigue (9.9 versus -1.0; P < 0.05) than nonusers. Compared with nonusers in subgroup analysis, users of alternative medical treatments, prayer, vitamin supplements, mushrooms, or rice and cereal reported clinically significant worse changes in some HRQOL subscales. CONCLUSION: While CAM did not provide any definite survival benefit, CAM users reported clinically significant worse HRQOLs.


Asunto(s)
Terapias Complementarias , Neoplasias/terapia , Calidad de Vida , Enfermo Terminal , Anciano , Estudios de Cohortes , Terapias Complementarias/psicología , Femenino , Estado de Salud , Humanos , Masculino , Neoplasias/mortalidad , Neoplasias/psicología , Estudios Prospectivos , Encuestas y Cuestionarios , Tasa de Supervivencia , Resultado del Tratamiento
4.
Horm Metab Res ; 44(4): 268-72, 2012 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-22351479

RESUMEN

Allantoin, an active principle of the yam, belongs to the group of guanidinium derivatives and has been reported to lower plasma glucose in diabetic animals. Recent evidence indicates that activation of the imidazoline I(2B) receptor (I(2B)R) by guanidinium derivatives also increases glucose uptake; however, the effect of allantoin on I(2B)R is still unknown. Glucose uptake into cultured C2C12 cells was determined using 2-[¹4C]-deoxy-D-glucose as a tracer. The changes in 5'-AMP-activated protein kinase (AMPK) expression were also identified by Western blotting analysis. The allantoin-induced glucose uptake action was dose-dependently blocked by BU224, a specific I2R antagonist, in C2C12 cells. Moreover, AMPK phosphorylation by allantoin was found to be dose-dependently increased in C2C12 cells using AICAR treatment as a reference. In addition, both actions of allantoin, the increases in glucose uptake and AMPK phosphorylation, were dose-dependently attenuated by amiloride in C2C12 cells. Moreover, compound C at concentrations sufficient to inhibit AMPK blocked the allantoin-induced glucose uptake and AMPK phosphorylation. Thus, we suggest that allantoin can activate I(2B)R to increase glucose uptake into cells, and propose I(2B)R as a new target for diabetic therapy.


Asunto(s)
Alantoína/farmacología , Glucosa/metabolismo , Receptores de Imidazolina/metabolismo , Extractos Vegetales/farmacología , Regulación hacia Arriba/efectos de los fármacos , Proteínas Quinasas Activadas por AMP/genética , Proteínas Quinasas Activadas por AMP/metabolismo , Animales , Transporte Biológico/efectos de los fármacos , Línea Celular , Dioscorea/química , Humanos , Receptores de Imidazolina/genética , Ratones , Músculo Esquelético/efectos de los fármacos , Músculo Esquelético/metabolismo
5.
Food Chem Toxicol ; 48(5): 1222-9, 2010 May.
Artículo en Inglés | MEDLINE | ID: mdl-20156520

RESUMEN

In the present study, the chemical constituents of Artemisia fukudo essential oil (AFE) were investigated using GC-MS. The major constituents were alpha-thujone (48.28%), beta-thujone (12.69%), camphor (6.95%) and caryophyllene (6.01%). We also examined the effects of AFE on the production of nitric oxide (NO), prostaglandin E(2) (PGE(2)), tumour necrosis factor (TNF)-alpha, interleukin (IL)-1beta, and IL-6, in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. Western blotting and RT-PCR tests indicated that AFE has potent dose-dependent inhibitory effects on pro-inflammatory cytokines and mediators. We investigated the mechanism by which AFE inhibits NO and PGE(2) by examining the level of nuclear factor-kappaB (NF-kappaB) activation within the mitogen-activated protein kinase (MAPK) pathway, which is an inflammation-induced signal pathway in RAW 264.7 cells. AFE inhibited LPS-induced ERK, JNK, and p38 phosphorylation. Furthermore, AFE inhibited the LPS-induced phosphorylation and degradation of Ikappa-B-alpha, which is required for the nuclear translocations of the p50 and p65 NF-kappaB subunits in RAW 264.7 cells. Our results suggest that AFE might exert an anti-inflammatory effect by inhibiting the expression of pro-inflammatory cytokines. Such an effect is mediated by a blocking of NF-kappaB activation which consequently inhibits the generation of inflammatory mediators in RAW264.7 cells. AFE may be useful for treating inflammatory diseases.


Asunto(s)
Antiinflamatorios no Esteroideos/farmacología , Artemisia/química , Macrófagos/efectos de los fármacos , Proteínas Quinasas Activadas por Mitógenos/antagonistas & inhibidores , FN-kappa B/antagonistas & inhibidores , Aceites Volátiles/farmacología , Aceites de Plantas/farmacología , Animales , Antiinflamatorios no Esteroideos/química , Línea Celular , Proliferación Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Citocinas/metabolismo , Regulación hacia Abajo , Cromatografía de Gases y Espectrometría de Masas , Expresión Génica , Perfilación de la Expresión Génica , Mediadores de Inflamación/antagonistas & inhibidores , Mediadores de Inflamación/fisiología , Macrófagos/inmunología , Macrófagos/metabolismo , Ratones , Proteínas Quinasas Activadas por Mitógenos/genética , FN-kappa B/genética , Aceites Volátiles/química , Aceites de Plantas/química , ARN Mensajero/metabolismo
6.
Diabet Med ; 25(12): 1473-7, 2008 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-19046248

RESUMEN

AIM: To examine the effect of cranberry ingestion on lipid profiles in Type 2 diabetic patients taking oral glucose-lowering drugs. METHODS: Thirty Type 2 diabetic subjects (16 males and 14 females; mean age 65 +/- 1 years) who were taking oral glucose-lowering medication regularly were enrolled in this randomized, placebo-controlled, double-blind study. Changes in lipid profiles, oxidized low-density lipoprotein (ox-LDL), glycaemic control, components of the metabolic syndrome, C-reactive protein (CRP) and urinary albumin excretion (UAE) were assessed after cranberry or placebo treatment for 12 weeks. RESULTS: Low-density lipoprotein (LDL) cholesterol decreased significantly in the cranberry group (from 3.3 +/- 0.2 to 2.9 +/- 0.2 mmol/l, P = 0.005) and the decrease was significantly greater than that in the placebo group (-0.4 +/- 0.1 vs. 0.2 +/- 0.1 mmol/l, P < 0.001). Total cholesterol and total : high-density lipoprotein (HDL) cholesterol ratio also decreased significantly (P = 0.020 and 0.044, respectively) in the cranberry group and the reductions were significantly different from those in the placebo group (P < 0.001 and P = 0.032, respectively). However, ox-LDL levels did not change significantly in response to cranberry consumption. Neither fasting glucose nor glycated haemoglobin improved in either group. Changes in components of the metabolic syndrome, UAE and CRP were not significantly different between groups. CONCLUSIONS: Cranberry supplements are effective in reducing atherosclerotic cholesterol profiles, including LDL cholesterol and total cholesterol levels, as well as total : HDL cholesterol ratio, and have a neutral effect on glycaemic control in Type 2 diabetic subjects taking oral glucose-lowering agents.


Asunto(s)
Diabetes Mellitus Tipo 2/sangre , Metabolismo de los Lípidos/efectos de los fármacos , Extractos Vegetales/farmacología , Vaccinium macrocarpon , Anciano , Humanos , Masculino , Fitoterapia , Factores de Riesgo
7.
Calcif Tissue Int ; 71(1): 88-94, 2002 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-12073158

RESUMEN

Safflower (Carthamus tinctorius L.) seeds have long been clinically used in Korea to promote bone formation and prevent osteoporosis. However, the beneficial effect has not been scientifically evaluated. Thus, in the present study we investigated whether phytoestrogen rich safflower seeds reduce bone loss in ovariectomized rats. Female Sprague-Dawley rats were subjected to bilateral ovariectomy or sham surgery. One week after the operation, ovariectomized rats were either fed a diet containing defatted safflower seeds or injected with 17b-estradiol (E2) for 4 weeks. As expected, ovariectomy resulted in a dramatic reduction in trabecular bone mass of the proximal tibia, increase in deposition of marrow fat, and in uterine atrophy. E2 treatment almost completely prevented bone loss as well as marrow adiposity, as examined by scanning electron microscopy and histomorphometry. Safflower seeds partially prevented ovariectomy-induced bone loss and slightly reduced marrow adiposity. Safflower seeds, in contrast to E2, exerted very weak uterotrophic action. In an attempt to elucidate the underlying mechanisms, effect of polyphenolic compounds extracted from safflower seeds on proliferation of osteoblast-like cells was also assessed in vitro. The mixed polyphenolic compounds stimulated growth of ROS 17/2.8 osteoblast-like cells in a dose-dependent manner (5-100 mg/ml), as potently as E2 and genistein. The present data provide the first direct in vivo evidence that safflower seeds have a protecting effect on bone loss caused by estrogen deficiency, without substantial effect on the uterus. The beneficial effect of safflower seeds may be mediated, at least in part, by the stimulating effect of polyphenolic compounds on proliferation of osteoblasts.


Asunto(s)
Carthamus tinctorius , Osteoporosis/prevención & control , Fitoterapia , Extractos Vegetales/uso terapéutico , Semillas , Alimentación Animal , Animales , Peso Corporal , Dieta , Femenino , Microscopía Electrónica de Rastreo , Tamaño de los Órganos , Osteoporosis/patología , Ovariectomía , Ratas , Ratas Sprague-Dawley , Tibia/efectos de los fármacos , Tibia/ultraestructura , Útero/anatomía & histología
8.
J Diabetes Complications ; 15(2): 97-102, 2001.
Artículo en Inglés | MEDLINE | ID: mdl-11274906

RESUMEN

Calcium supplementation is important in the treatment of osteoporosis, a disease that may also occur in diabetic patients. The acute effects of calcium supplementation and their relationship to gastric emptying time, however, have rarely been studied in type 2 diabetic patients. We evaluated the acute biochemical variations induced by the administration of two different calcium preparations, calcium citrate and calcium carbonate, in 16 (male/female: 13/3) Chinese diabetic patients. Serum free calcium, intact parathyroid hormone (i-PTH), and amount of urinary excretion of calcium (uCal/uCr) were evaluated after a single dose of 1200 mg of elemental calcium in each preparation. The free calcium levels did not change significantly in either group. However, significant suppression of i-PTH after calcium citrate administration at 1 h (17.1+/-2.0 pg/ml, P=.023), and after calcium carbonate administration at 2 h (14.2+/-2.5 pg/ml, P=.000), was noted when compared with individual basal level (21.2+/-2.5 and 19.3+/-2.4 pg/ml, respectively). The suppressive effect on i-PTH lasted for 6 h after calcium citrate and 5 h after calcium carbonate preparation of the 6-h study period. After administration of calcium citrate, the uCal/uCr of 2-to-4-h collection was significantly higher than that of the basal and 0-to-2-h collections: 0.25+/-0.04 vs. 0.19+/-0.03, P=.025; and 0.25+/-0.04 vs. 0.19+/-0.02, P=.014, respectively. A similar finding was observed for calcium carbonate: 0.23+/-0.03 vs. 0.18+/-0.02, P=.019; and 0.23+/-0.03 vs. 0.18+/-0.02, P=.011, respectively. We conclude that, in this group of Chinese type 2 diabetic patients in our study, the oral administration of 1200 mg elemental calcium in either calcium citrate or calcium carbonate preparation can induce a significant suppression of i-PTH. This may be helpful in preventing or treating osteoporosis. A prolonged gastric emptying time in these diabetic subjects may contribute to the non-significant alteration in free calcium levels after the administration of either calcium preparation.


Asunto(s)
Carbonato de Calcio/farmacología , Citrato de Calcio/farmacología , Calcio/metabolismo , Diabetes Mellitus Tipo 2/fisiopatología , Vaciamiento Gástrico , Absorción Intestinal , Administración Oral , Anciano , Pueblo Asiatico , Glucemia/metabolismo , Calcio/sangre , Carbonato de Calcio/administración & dosificación , Citrato de Calcio/administración & dosificación , Creatinina/sangre , Diabetes Mellitus Tipo 2/sangre , Femenino , Hemoglobina Glucada/análisis , Humanos , Masculino , Persona de Mediana Edad , Hormona Paratiroidea/sangre , Fosfatos/sangre , Taiwán
9.
J Formos Med Assoc ; 99(6): 510-2, 2000 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-10925560

RESUMEN

The success of mini-laparoscopic cholecystectomy and splenectomy has encouraged the application of this new technique in children with non-complicated intussusception. Here, we report on the use of this technique in two children, aged 21 and 24 months, respectively, who had failed saline enema reduction of ileocolic intussusception. Under a 2-mm videoscopic visualization, the intussuscepted bowel was identified and reduced with the assistance of a 2-mm working port placed at the right lower abdominal area and a 5- to 10-mm working port placed at the supraumbilical area. An intussuscepted lymph node at the ileocecal area, as the lead point, was removed at the time of the procedure in one child. Both patients experienced relief of symptoms the day after operation and resumed a regular diet on the first post-operative day. No recurrence or complications, including abscess and wound infection, occurred. These findings suggest that mini-laparoscopic reduction is a safe procedure for children with uncomplicated intussusception. Moreover, the procedure provides better cosmesis than conventional laparoscopic techniques.


Asunto(s)
Intususcepción/cirugía , Preescolar , Humanos , Lactante , Laparoscopía , Masculino
10.
Blood ; 93(11): 3893-9, 1999 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-10339498

RESUMEN

Extracellular signal-regulated kinase (ERK) is an important intermediate in signal transduction pathways that are initiated by many types of cell surface receptors. It is thought to play a pivotal role in integrating and transmitting transmembrane signals required for growth and differentiation. Constitutive activation of ERK in fibroblasts elicits oncogenic transformation, and recently, constitutive activation of ERK has been observed in some human malignancies, including acute leukemia. However, mechanisms underlying constitutive activation of ERK have not been well characterized. In this study, we examined the activation of ERK in 79 human acute leukemia samples and attempted to find factors contributing to constitutive ERK activation. First, we showed that ERK and MEK were constitutively activated in acute leukemias by in vitro kinase assay and immunoblot analysis. However, in only one half of the studied samples, the pattern of ERK activation was similar to that of MEK activation. Next, by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and immunoblot analysis, we showed hyperexpression of ERK in a majority of acute leukemias. In 17 of 26 cases (65.4%) analyzed by immunoblot, the pattern of ERK expression was similar to that of ERK activation. The fact of constitutive activation of ERK in acute leukemias suggested to us the possibility of an abnormal downregulation mechanism of ERK. Therefore, we examined PAC1, a specific ERK phosphatase predominantly expressed in hematopoietic tissue and known to be upregulated at the transcription level in response to ERK activation. Interestingly, in our study, PAC1 gene expression in acute leukemias showing constitutive ERK activation was significantly lower than that in unstimulated, normal bone marrow (BM) samples showing minimal or no ERK activation (P =.002). Also, a significant correlation was observed between PAC1 downregulation and phosphorylation of ERK in acute leukemias (P =.002). Finally, by further analysis of 26 cases, we showed that a complementary role of MEK activation, ERK hyperexpression, and PAC1 downregulation could contribute to determining the constitutive activation of ERK in acute leukemia. Our results suggest that ERK is constitutively activated in a majority of acute leukemias, and in addition to the activation of MEK, the hyperexpression of ERK and downregulation of PAC1 also contribute to constitutive ERK activation in acute leukemias.


Asunto(s)
Proteínas Quinasas Dependientes de Calcio-Calmodulina/metabolismo , Leucemia/enzimología , Quinasa 1 de Quinasa de Quinasa MAP , Proteínas Serina-Treonina Quinasas/metabolismo , Proteínas Tirosina Fosfatasas/metabolismo , Regulación hacia Abajo , Fosfatasa 2 de Especificidad Dual , Activación Enzimática , Regulación Neoplásica de la Expresión Génica , Humanos , Proteína Fosfatasa 2 , Proteínas Serina-Treonina Quinasas/genética , Proteínas Tirosina Fosfatasas/genética , Células Tumorales Cultivadas , Regulación hacia Arriba
11.
Abdom Imaging ; 24(2): 165-70, 1999.
Artículo en Inglés | MEDLINE | ID: mdl-10024404

RESUMEN

BACKGROUND: Iodized-oil computed tomography (CT) is useful for the diagnosis of hepatocellular carcinoma, but there may be false-positive results in patients who have undergone some percutaneous transhepatic interventional procedures. The purpose of the present study was to verify the correlation between subsegmental hepatic parenchymal retention of iodized oil on CT and the arterioportal shunt caused by liver biopsy. METHODS: Iodized-oil CT scans were reviewed in 24 patients with hepatic tumors who had liver biopsy and subsequent iodized oil chemoembolization. Iodized oil chemoembolization was performed shortly after biopsy (1-10 days; mean = 2.6 days). The results were correlated with hepatic arteriography, with a special emphasis on the presence of hepatic arterioportal shunt. RESULTS: Wedge-shaped subsegmental retention of iodized oil along or adjacent to the biopsy needle path was observed in iodized-oil CT in 17 of the 24 patients. In three patients, there was subsegmental enhancement on prebiopsy helical dynamic liver CT at the same area of iodized oil retention, and therefore iodized oil retention was considered to be due to hepatocellular carcinoma. In the remaining 13 (54%) patients, the peripheral iodized oil retention was considered to be due to biopsy-induced arterioportal shunt. In all these patients, arterioportal shunt was confirmed by hepatic arteriography. CONCLUSION: Wedge-shaped hepatic parenchymal retention of iodized oil is commonly observed in iodized-oil CT due to biopsy-induced arterioportal shunt, and this appearance should not be confused with a hepatocellular carcinoma.


Asunto(s)
Fístula Arteriovenosa/diagnóstico por imagen , Fístula Arteriovenosa/etiología , Carcinoma Hepatocelular/diagnóstico por imagen , Aceite Yodado , Neoplasias Hepáticas/diagnóstico por imagen , Tomografía Computarizada por Rayos X , Biopsia con Aguja/efectos adversos , Carcinoma Hepatocelular/terapia , Quimioembolización Terapéutica , Medios de Contraste , Reacciones Falso Positivas , Femenino , Arteria Hepática/diagnóstico por imagen , Arteria Hepática/lesiones , Humanos , Hígado/irrigación sanguínea , Hígado/patología , Neoplasias Hepáticas/terapia , Masculino , Persona de Mediana Edad , Vena Porta/diagnóstico por imagen , Vena Porta/lesiones
12.
Cancer ; 82(9): 1626-31, 1998 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-9576280

RESUMEN

BACKGROUND: In the past 4 years, the weekly 24-hour infusion of high dose 5-fluorouracil (5-FU) and leucovorin in the treatment of patients with advanced gastric carcinoma has been prospectively studied at the authors' institution. This has enabled them to explore the possibility that the level of expression of thymidylate synthase (TS), the target enzyme of 5-FU, is related to the drug sensitivity of gastric carcinoma to 5-FU-based chemotherapy. METHODS: To be eligible for this study, patients were required to have received high dose 5-FU and leucovorin chemotherapy (weekly 24-hour infusions of 5-FU, 2,600 mg/m2, and leucovorin, 300 mg/m2) and to have had adequate prechemotherapy gastric carcinoma tissues for immunohistochemical study. TS106 monoclonal antibody was used to detect the expression of TS. A visual scoring system, which ranged from 0 to 3+, was adopted by 2 independent pathologists to semiquantitate the intensity of TS expression. RESULTS: Between 1993 and 1996, a total of 30 patients, 18 men and 12 women, with a median age of 61.5 years, were enrolled. Of these patients, 16 (53.3%) and 14 (46.7%) had high and low expression of TS, respectively. Two of the 16 patients (12.5%) with high expression of TS and 13 of the 14 patients (92.9%) with low expression of TS responded to chemotherapy (P < 0.001, chi-square test). The median overall survival was 10 months for patients with low TS expression and 4 months for patients with high TS expression (P < 0.01, log rank test). CONCLUSIONS: The data from this study suggest that the expression of TS, as determined by immunohistochemistry, is a relatively reliable indicator of whether 5-FU should be used in the treatment of patients with gastric carcinoma.


Asunto(s)
Adenocarcinoma/tratamiento farmacológico , Adenocarcinoma/enzimología , Antimetabolitos Antineoplásicos/uso terapéutico , Fluorouracilo/uso terapéutico , Neoplasias Gástricas/tratamiento farmacológico , Neoplasias Gástricas/enzimología , Timidilato Sintasa/biosíntesis , Adulto , Anciano , Relación Dosis-Respuesta a Droga , Resistencia a Antineoplásicos , Femenino , Humanos , Inmunohistoquímica , Infusiones Intravenosas , Masculino , Persona de Mediana Edad
13.
Insect Mol Biol ; 7(1): 41-50, 1998 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-9459428

RESUMEN

A cDNA clone for prophenoloxidase was isolated from the most important human malaria vector, Anopheles gambiae. The clone encoded a polypeptide of 79341 Da that contains the two copper binding domains common to all invertebrate prophenoloxidases and haemocyanins. Expression of the prophenoloxidase gene was detected throughout all life stages from egg to imago in two strains of A. gambiae; however, the strongest expression was observed in developing embryos in eggs. The prophenoloxidase gene was mapped to the inversion rich region of the right arm of chromosome-2 in region 13B.


Asunto(s)
Anopheles/enzimología , Catecol Oxidasa/genética , Mapeo Cromosómico , Precursores Enzimáticos/genética , Genes de Insecto , Insectos Vectores/enzimología , Secuencia de Aminoácidos , Animales , Anopheles/genética , Secuencia de Bases , Gatos , Clonación Molecular , ADN Complementario , Expresión Génica , Humanos , Proteínas de Insectos/genética , Insectos Vectores/genética , Malaria/transmisión , Datos de Secuencia Molecular , ARN Mensajero , Conejos , Homología de Secuencia de Aminoácido
14.
Insect Biochem Mol Biol ; 27(11): 983-92, 1997 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-9537761

RESUMEN

Two kinds of cDNA clones encoding prophenoloxidases (ProPO; zymogen of phenoloxidase (monophenol, L-dopa: oxygen oxydoreductase, EC 1.14.18.1)) were isolated by polymerase chain reaction (PCR) followed by screening of cDNA library that was prepared from whole larvae of the fall webworm, Hyphantria cunea (Lepidoptera, Arctiidae). The cDNAs encode 681 and 697 amino acids with molecular masses of 78.2 and 80.2 kDa, respectively. Deduced amino acid sequence homology between the two H. cunea ProPOs are only 49% whereas the homology against other insect ProPOs ranged from about 40 to 72%. The phylogenic analysis showed that the insect ProPOs are grouped mainly into two families. A putative proteolytic cleavage site for enzyme activation was identical to other insect ProPOs. The conserved copper binding sites were 84-62% homologous to arthropod ProPOs. Two additional highly conserved regions were found in the carboxy terminal. Furthermore, like other insect prophenoloxidases, hydrophobic signal peptide sequences were absent in the deduced ProPOs from H. cunea. Southern blot analysis indicated that the H. cunea ProPO1 is present as a single copy in the genome. Northern blot analysis showed that the expression of the ProPO genes were concentrated in mid-instar larvae, but were much lower in other developmental stages.


Asunto(s)
Catecol Oxidasa/genética , Precursores Enzimáticos/genética , Lepidópteros/enzimología , Secuencia de Aminoácidos , Animales , Sitios de Unión , Catecol Oxidasa/biosíntesis , Catecol Oxidasa/química , Clonación Molecular , Secuencia Conservada , ADN Complementario , Precursores Enzimáticos/biosíntesis , Precursores Enzimáticos/química , Lepidópteros/genética , Datos de Secuencia Molecular , Peso Molecular , Filogenia , Señales de Clasificación de Proteína/química , Proteínas Recombinantes/biosíntesis , Proteínas Recombinantes/química , Alineación de Secuencia , Homología de Secuencia de Aminoácido
15.
Regul Pept ; 67(2): 115-21, 1996 Dec 03.
Artículo en Inglés | MEDLINE | ID: mdl-8958582

RESUMEN

The effects of dietary sodium intake on the gene expression of the renin-angiotensin system (RAS) were investigated in rat central and peripheral tissues in a single set of experiment. Northern and reverse transcriptase-polymerase chain reaction (RT-PCR) techniques were used to detect mRNA expression in rats fed a low- or a high-sodium diet (5 or 500 mmol Na+/kg diet) for 20 days. Plasma and renal renin levels were elevated in rats maintained on the low-sodium diet. Sodium deprivation enhanced the expression of angiotensinogen, renin, AT1A and AT1B receptor subtypes in the hypothalamus, but suppressed them in the brainstem. Kidney and adrenal levels of those mRNAs were also enhanced in the sodium-restricted rats. Both AT1A and AT1B mRNAs changed in a similar magnitude in each tissue examined upon dietary sodium intake. AT1A was the predominant receptor subtype of AT1 in all the tissues examined in the present study except the adrenal gland. The present study demonstrated that dietary sodium modulated the gene expression of the RAS components in the central and peripheral tissues. It also showed that the RAS components in the brainstem and hypothalamus were differentially expressed upon sodium deprivation. This suggests different roles of the RAS in these tissues in maintaining body fluid homeostasis in response to different sodium intakes.


Asunto(s)
Sistema Renina-Angiotensina/efectos de los fármacos , Sistema Renina-Angiotensina/fisiología , Sodio en la Dieta/farmacología , Glándulas Suprarrenales/metabolismo , Glándulas Suprarrenales/ultraestructura , Angiotensinógeno/biosíntesis , Animales , Northern Blotting , Tronco Encefálico/metabolismo , Tronco Encefálico/ultraestructura , Dieta , Relación Dosis-Respuesta a Droga , Expresión Génica/efectos de los fármacos , Hipotálamo/metabolismo , Hipotálamo/ultraestructura , Riñón/metabolismo , Riñón/ultraestructura , Masculino , Reacción en Cadena de la Polimerasa , ARN Mensajero/metabolismo , Ratas , Ratas Wistar , Receptor de Angiotensina Tipo 1 , Receptores de Angiotensina/biosíntesis , Renina/sangre , Renina/metabolismo , Sodio en la Dieta/administración & dosificación , Transcripción Genética
16.
Radiology ; 201(1): 221-5, 1996 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-8816547

RESUMEN

PURPOSE: To assess whether color Doppler ultrasonography (US) can help diagnose early acute appendicitis in appendices of equivocal size at gray-scale US. MATERIALS AND METHODS: In a retrospective study, color Doppler US findings were evaluated in 20 individuals with proved normal appendix at barium enema examination and in 50 patients in whom pathologic findings confirmed acute appendicitis. In a prospective study, the diagnostic value of hyperemia in the wall of the appendix in differentiating a normal from an inflamed appendix was evaluated in 26 patients with appendices of equivocal size (5-7 mm in maximal outer diameter) at grayscale US. RESULTS: In the retrospective study, 44 of the 50 patients had hyperemia at color Doppler US. The remaining six patients had no hyperemia, and pathologic findings confirmed gangrenous appendices. The 20 patients with proved normal appendix at barium enema examination had no detectable blood flow in the wall. In the prospective study, 10 of 26 patients had hyperemia at color Doppler US, and pathologic findings confirmed early acute appendicitis. Results of barium enema examination (n = 12) or clinical follow-up (n = 4) confirmed no appendicitis in the remaining 16 patients. CONCLUSION: Hyperemia in the wall of the appendix at color Doppler US is a sensitive indicator for inflammation. A simple additional color Doppler US examination may be helpful in the diagnosis of early acute appendicitis when an appendix is well depicted and is equivocal in size at gray-scale US.


Asunto(s)
Apendicitis/diagnóstico por imagen , Ultrasonografía Doppler en Color , Enfermedad Aguda , Adulto , Apéndice/irrigación sanguínea , Apéndice/diagnóstico por imagen , Sulfato de Bario , Estudios de Casos y Controles , Medios de Contraste , Humanos , Hiperemia/diagnóstico por imagen , Masculino , Estudios Prospectivos , Radiografía , Estudios Retrospectivos
17.
Urol Clin North Am ; 10(4): 719-27, 1983 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-6636385

RESUMEN

The authors review the indications, techniques, and complications of percutaneous stone removal. Step-by-step descriptions of each technique are provided.


Asunto(s)
Cálculos Renales/cirugía , Irrigación Terapéutica , Analgesia , Anestesia Local , Dilatación , Endoscopía , Fístula , Fluoroscopía , Humanos , Cálculos Renales/tratamiento farmacológico , Pelvis Renal/cirugía , Cuidados Preoperatorios , Soluciones , Cálculos Ureterales/cirugía
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