Mechanism of YY1 mediating autophagy dependent ferroptosis in PM2.5 induced cardiac fibrosis.

Epidemiological studies have demonstrated strong associations between exposure to ambient fine particulate matter (PM2.5) and cardiac disease. To investigate the potential mechanism of cardiac fibrosis induced by PM2.5, we established PM2.5 exposure models in vivo and in vitro, and then cardiac fibrosis was evaluated. The ferroptosis and ferritinophagy was detected to characterize the effects of PM2.5 exposure. The results indicated that PM2.5 exposure could induce cardiac fibrosis in mice. YY1 was induced by PM2.5 exposure and then increased NCOA4, a cargo receptor for ferritinophagy, which interacted with FHC and promoted the transport of ferritin to the autophagosome for degradation. The release of large amounts of free iron from ferritinophagy led to lipid peroxidation directly via the Fenton reaction, thereby triggering ferroptosis. Moreover, siNCOA4 could partly restore the FHC protein level in HL-1 cells and inhibit the occurrence of downstream ferroptosis. Functionally, NCOA4 knockdown inhibited ferroptosis and alleviated HL-1 cell death induced by PM2.5. Ferroptosis inhibitor (Ferrostatin-1) could reverse the promoting effect of ferritinophagy mediated ferroptosis on cardiac fibrosis induced by PM2.5 exposure in mice. Our study indicated that PM2.5 induced cardiac fibrosis through YY1 regulating ferritinophagy-dependent ferroptosis.

Influence and Action Mechanisms of Governmental Relations Embeddedness for Fostering Green Production Demonstration Household: Evidence from Shaanxi, Sichuan, and Anhui Province, China.

As an innovative tactic, the core aspects of green products should be comprehensively demonstrated and firmly promoted to enhance their adoption. For doing so, continuous governmental support and interventions through distinct sets of networking and relationships could be crucial for synthesizing and diffusing the extent of green production demonstration households. Interestingly, the structural relationship between these two has not yet been evaluated comprehensively by the existing literature. Therefore, the study empirically analyzes the impact and mechanism of government relationships embedded in fostering green production demonstration households. The study compiles the empirical data from 963 farmers which were collected from the major tea-producing areas of Shaanxi, Sichuan, and Anhui provinces, China. In order to craft the findings, first we constructed the ordered Probit for benchmark regression analysis. Meanwhile, the Ordinary Standard Error Ordered Probit model, Ordered Logit model, and multivariate linear model were constructed for the robustness test. Third, the Extended Ordered Probit model and Bootstrap mediation effect model were used to test the path diagram. Finally, robustness testing and endogeneity processing test were used to explore the reliability of the findings. The results showed that: (i) Government relationship embedding has a positive effect on fostering green production demonstration households. In particular, factors such as relationships with general government staff, professional and technical personnel, and village cadre are most significant. (ii) Seemingly, the heterogeneity analysis shows that the farmers with large operating scales and low family economic status have a relatively stronger impact. (iii) Further mechanism research results show that government relations are embedded through government identification (policy identification, government trust), improving farmers' behavioral ability (production knowledge reserve, self-efficacy), and strengthening farmers' perceived value of green production (self-interest perception, altruistic values). Therefore, the government should strengthen the interactive mechanism embedded with farm households and extend support for green production demonstration zones. The farmers' information-sharing facilities and platforms should be modernized and highlighted according to the local conditions and long-term targeted strategies.

Effects of Market Incentives and Livelihood Dependence on Farmers' Multi-Stage Pesticide Application Behavior-A Case Study of Four Provinces in China.

Improvement in pesticide application and efficiency structure has long been recognized as having great significance in reducing pollution, ensuring food safety, and promoting green agricultural development. Based on theoretical analysis, using the survey data of 766 farmers in key tea areas in Shaanxi, Sichuan, Zhejiang, and Anhui provinces in China, the study empirically analyzes the influence of market incentives and livelihood dependence on farmers' multi-stage pesticide application behavior. More specifically, the study employed ordered probit analysis to craft its findings. The dependent variable of this study is the multi-stage pesticide application problem of farmers, and the core independent variables are market incentives and livelihood dependence, and the judgment is based on the core variable coefficients of the econometric model of farmers at each stage. The study found the following: (i) Market incentives significantly prompted some farmers to give up synthetic pesticide application and farmers tend to choose green pesticides in the type of pesticide application. (ii) Livelihood dependence meant that the proportion of tea income significantly prompts farmers to apply pesticides, and also creates a tendency for farmers to choose green and low-toxic pesticides in the type of pesticide application. The planting period tends to have a moderate impact on applying green and low-toxic pesticides. (iii) The interaction term of market incentives and the proportion of tea income has no significant impact on farmers' multi-stage pesticide application behavior. The interaction term of market incentives and planting years has impacted negatively on whether farmers apply pesticides, and has no significant impact on farmers' choice of pesticide application types, but makes farmers increase the amount of green and low-toxic pesticides. (iv) The education level of the household head significantly promotes farmers to choose green and low-toxic pesticides. Seemingly, the brand effect of pesticides significantly encourages farmers to choose green and low-toxic pesticides. In external support, technical training significantly encourages farmers to choose green and low-toxic pesticides. Furthermore, better infrastructure and local market conditions significantly encourage farmers to reduce the use of conventional pesticides.

Shenjing Guben Wan promotes sperm development by increasing the activity of seminiferous epithelium Sertoli cells.

Background: Infertility is an important social problem. Asthenozoospermia (AZS) is a common pathological cause of male infertility, but its pathogenesis is unclear. Shenjing Guben Wan (SJGBW), a traditional Chinese medicine, has shown remarkable effects during the clinical treatment of oligozoospermia or AZS. Methods: In this study, clinical evaluations were carried out on 184 AZS patients receiving SJGBW treatment, including sperm count, sperm quality, and pregnancy rate. Also, ornidazole was used to build an AZS mouse model, and SJGBW treatment was administered. The sperm quantity and fertility of mice in different groups were evaluated; a cholecystokinin octapeptide-8 (CCK-8) experiment was carried out to test the activity of seminiferous epithelium Sertoli cells, and immunohistochemistry and the Terminal Deoxynucleotidyl Transferase-mediated dUTP Nick-End Labeling (TUNEL) method were employed to test the pathological information and expression of the Sertoli cell surface marker in the testicular tissues of mice in each group. Results: The sperm vitality, progressive sperm motility, and sperm morphology of patients who received SJGBW treatment were all improved (P<0.05). In the AZS group, the average sperm count, sperm vitality, pregnancy rate, and female mouse litters were all lower relative to mice in the control group. Following SJGBW treatment, the average sperm count, sperm vitality, pregnancy rate, and female mouse litters of mice in the AZS group were all significantly improved. The cytobiological experimental results showed that compared with the serum of normal male mice in the control group, the drug serum containing SJGBW could improve the cell vitality and proliferative ability of seminiferous epithelium Sertoli cells in AZS mice. Furthermore, the TUNEL results showed that the seminiferous tubule Sertoli cells and mesenchymal cells of the AZS mice exhibited the most significant apoptosis, which was alleviated following SJGBW treatment. Moreover, the levels of Sertoli cell marker, SOX9, and anti-apoptosis protein, Bcl2, in SJGBW-treated mice were both higher than that in AZS mice. Conclusions: SJGBW can promote the development and maturation of germ cells by facilitating the proliferation of Sertoli cells in AZS patients, thereby improving the fertility of these patients.