RESUMEN
Cordyceps guangdongensis as a kind of fungus, has been discovered and cultivated successfully in recent years. However, its safety assessments have not been studied. In this report, a serial of tests for toxicological safety assessments were depicted in details. These tests included bacterial reverse mutation (Ames) study, bone marrow cell micronucleus test in mice, sperm aberration test in mice, teratogenicaction test in rats, acute toxicity test and 13-week oral toxicity study in rats. After a profound analysis of these tests, it clearly demonstrated that C. guangdongensis did not have any mutagenic, clastogenic nor genotoxic effects; the oral LD50 of the biomass in rats was greater than 15 g/kg body weight; the no-observed adverse-effect-levels (NOAEL) was 5.33 g/kg body weight according to the 13-week oral toxicity analysis. Therefore, a conclusion can be drawn that C. guangdongensis is considered safe for long term consumption.
Asunto(s)
Cordyceps/química , Mutágenos/toxicidad , Extractos Vegetales/toxicidad , Teratógenos/toxicidad , Pruebas de Toxicidad/métodos , Administración Oral , Animales , Embrión de Mamíferos/efectos de los fármacos , Embrión de Mamíferos/embriología , Desarrollo Embrionario/efectos de los fármacos , Femenino , Masculino , Exposición Materna , Ratones , Mutágenos/clasificación , Nivel sin Efectos Adversos Observados , Extractos Vegetales/clasificación , Embarazo , Ratas , Medición de Riesgo , Salmonella typhimurium/efectos de los fármacos , Salmonella typhimurium/genética , Espermatozoides/efectos de los fármacos , Espermatozoides/patología , Teratógenos/clasificaciónRESUMEN
OBJECTIVE: To study the genetic polymorphism and intraspecific genetic differentiation of different populations of Pogostemon cablin, and find out the effective method to distinguish DNA fingerprint of different populations of P. cablin. METHOD: Five plant populations of P. cablin were analyzed by RAPD markers. PopGen 32 software for clustering analysis and calculating. Fourteen of the 80 random primers were tested to possess the stronger detecting effect of polymorphous character. RESULT: A total of 84 bands was amplified by the 10 primers, among them 17 bands were monomorphic. 67 of them were polymorphic. The results indicated that the genetic variations existed within the different plant populations of the same species. CONCLUSION: It is feasible by RAPD technique with specifically primer to analyze the genetic diversity and identify 5 plant populations of P. cablin. RAPD technique has provided a new path for identification and classification of P. cablin genetic germplasm.