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Métodos Terapéuticos y Terapias MTCI
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1.
Yao Xue Xue Bao ; 40(3): 225-30, 2005 Mar.
Artículo en Chino | MEDLINE | ID: mdl-15952593

RESUMEN

AIM: To explore the effect and the possible mechanism of ginsenoside Rb1 on beta-amyloid peptide (beta-AP)(25-35) -induced tau protein hyperphosphorylation in cortical neurons. METHODS: Western blotting and immunocytochemical staining were used to detect tau phosphorylation level, total tau and glycogen synthase kinase-3beta (GSK-3beta) in cortical neurons. RESULTS: After exposure to beta-AP(25-35) (20 micromol x L(-1)) for 12 h, the levels of tau protein phosphorylation in the sites of Ser 396, Ser 199/202, Thr 231 and total tau were raised. Meanwhile, the expression of GSK-3beta also increased. Pretreatment with ginsenoside Rbl or lithium chloride, a specific inhibitor of GSK-3beta, markedly reduced beta-AP(25-35)-induced tau hyperphosphorylation and the expression of GSK-3beta. CONCLUSION: Ginsenoside Rb1 can attenuate beta AP(25-35)-induced tau protein hyperphosphorylation in cortical neurons by inhibiting the expression of GSK-3beta.


Asunto(s)
Péptidos beta-Amiloides/antagonistas & inhibidores , Corteza Cerebral/metabolismo , Ginsenósidos/farmacología , Glucógeno Sintasa Quinasa 3/metabolismo , Fragmentos de Péptidos/antagonistas & inhibidores , Proteínas tau/metabolismo , Animales , Corteza Cerebral/citología , Femenino , Feto , Ginsenósidos/aislamiento & purificación , Glucógeno Sintasa Quinasa 3 beta , Neuronas/metabolismo , Panax/química , Fosforilación , Plantas Medicinales/química , Ratas , Ratas Sprague-Dawley
2.
Yao Xue Xue Bao ; 39(9): 673-6, 2004 Sep.
Artículo en Chino | MEDLINE | ID: mdl-15606011

RESUMEN

AIM: To explore the possible role of p21, cyclin E and cyclin-dependent kinase 2 (CDK2) in the protection of ginsenoside Rg1 against tert-butylhydroperoxide (t-BHP)-induced senescence in WI-38 cells. METHODS: The cellular ultrastructure, cytometric assay and beta-galactosidase (beta-gal) cytochemistry staining were used to evaluate cell senescence. The levels of of p21, cyclin E and CDK2 protein were detected by Western blot. RESULTS: Pretreatment with Rg1 significantly attenuated t-BHP-induced senescence in WI-38 cells. Simultaneously, compared with cells treated with t-BHP alone, Rg1 pretreatment markedly decreased the level of p21 protein and increased the levels of CDK2 and cyclin E. CONCLUSION: p21, cyclin E and CDK2 may be involved in the process of ginsenoside Rg1 protection against t-BHP-induced senescence in WI-38 cells.


Asunto(s)
Quinasas CDC2-CDC28/metabolismo , Senescencia Celular/efectos de los fármacos , Ciclina E/metabolismo , Ginsenósidos/farmacología , Proteínas Proto-Oncogénicas p21(ras)/metabolismo , Línea Celular , Quinasa 2 Dependiente de la Ciclina , Fibroblastos/citología , Fibroblastos/metabolismo , Ginsenósidos/aislamiento & purificación , Humanos , Panax/química , Plantas Medicinales/química , terc-Butilhidroperóxido/antagonistas & inhibidores
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