RESUMEN
OBJECTIVE: To explore the action mechanism of ginsenoside Rb1 (GRb1) on protecting herpes simplex virus-1 (HSV-1) infected nerves by studying its inhibitory effects on abnormal changes of apoptosis and nerve growth factor (NGF) mRNA expression in HSV-1 infected human glioma cells U251. METHODS: The inhibitory effects of GRb1 on HSV-1 induced abnormal apoptosis of U251 cells were detected using MTT colorimetry and flow cytometry. The NGF mRNA expressions in different treatment groups were detected using semiquantitative RT-PCR. RESULTS: (1) In 400 microg/mL GRb1 + HSV-1 group, MTT value was higher than HSV-1 group at 24, 36, and 48 h after infection (P < 0.05). (2) Cytopathic effects (CPE) were observed in HSV-1 group at 36 h after infection. In 400 microg/mL GRb1 + HSV-1 group merges increased at 36 h after infection, but most cells were in normal shapes. (3) Results of flow cytometry showed that the cell apoptosis rate was lower in 400 microg/mL GRb1 + HSV-1 group than in the HSV-1 group at24 and 36 h after infection (P < 0.05). (4) Results of RT-PCR showed that in 400 microg/mL GRb1 + HSV-1 group, NGF mRNA expressions decreased at 6-12 h after infection (P < 0.05), but it increased at 24, 36, and 48 h after infection, and was obviously higher than that in the HSV-1 group (P < 0.05). CONCLUSIONS: GRb1 at an appropriate concentration could inhibit abnormal cell apoptosis and changes of NGF mRNA expressions in HSV-1 infection. Therefore, we inferred that GRb1 could protect nerves possibly through up-regulating NGF mRNA expressions and inhibiting apoptosis.
Asunto(s)
Apoptosis/efectos de los fármacos , Ginsenósidos/farmacología , Herpes Simple/metabolismo , Factor de Crecimiento Nervioso/metabolismo , Línea Celular Tumoral , Herpesvirus Humano 1 , HumanosRESUMEN
OBJECTIVE: To evaluate the effect and acute toxicity of different extraction of Fructus Meliae Toosendan. METHODS: Samples were extracted from Fructus Meliae Toosendan using petroleum ether (I), ethyl acetate (II), 80% alcohol (III) or water (IV). Mices writhing response were induce by ip 0.9% acetic acid and foot ache were induced by sc formaldehyde. Mices feet or ears were smeated by sccarrageenin or dimethylbenzene. And the effects of these samples were evaluated on these models. RESULTS: The sample II 40 g/kg significantly decreased the writhing response induced by acetic acid and relieved mice foot pain induced by formaldehyde, inhibited the swelling of mice ear induced by dimethylbenzene. It markedly decreased the body weight and increased the index of testes in mice. The sample III 20 g/kg inhibited foot swelling induced by carrageenin and ear swelling induced by dimethylbenzene in mice. The sample I significantly relieved the foot pain induced by formaldehyde, increased the indexes of testis and adrenal gland in mice. The sample IV had no action on pain or inflammatory in mice. The LD50 of the sample II was 82.85 g/kg. And ig the sample I 133.2 g/kg, sample III 122.0 g/kg or sample IV 52.0 g/kg could not induce mice to death. CONCLUSION: The sample II has anti-inflammatory and antalgic action. But it also has serious acute toxicity. These results show that the efficacious component of Fructus Meilae Toosendan may exist in the ethyl acetate extraction, The toxicity and efficacious component may be the same material.