Influence of feeding thermally peroxidized soybean oil on growth performance, digestibility, gut integrity, and oxidative stress in nursery pigs.

The objectives of the current experiments were to evaluate the effect of feeding soybean oil (SO) with different levels of peroxidation on lipid, N, and GE digestibility, gut integrity, oxidative stress, and growth performance in nursery pigs. Treatments consisted diets containing 10% fresh SO (22.5 °C) or thermally processed SO (45 °C for 288 h, 90 °C for 72 h, or 180 °C for 6 h), each with an air infusion of 15 L/min, with postprocessing peroxide values of 7.6, 11.5, 19.1, and 13.4 mEq/kg and p-anisidine values of 1.92, 6.29, 149, and 159, for the 22.5 °C, 45 °C, 90 °C and 180 °C processed SO, respectively. In experiment 1, 64 barrows (7.1 ± 0.9 kg initial BW) were randomly allotted into 2 rooms of 32 pens and individually fed their experimental diets for 21 d, with a fresh fecal sample collected on day 20 for determination of GE and lipid digestibility. In experiment 2, 56 barrows (BW 9.16 ± 1.56 kg) were placed into individual metabolism crates for assessment of GE, lipid, and N digestibility and N retention. Urinary lactulose to mannitol ratio was assessed to evaluate in vivo small intestinal integrity, and urine and plasma were collected to analyze for markers of oxidative stress. Pigs were subsequently euthanized to obtain liver weights and analyze the liver for markers of oxidative stress. In experiment 1, pigs fed the SO thermally processed at 90 °C had reduced ADG (P = 0.01) and ADFI (P = 0.04) compared to pigs fed the other SO treatment groups, with no differences noted among pigs fed the 22.5 °C, 45 °C, and 180 °C SO treatments. No effects of feeding thermally processing SO on dietary GE or lipid digestibility (P > 0.10) were noted in either experiment. In experiment 2, there was no dietary effect of feeding peroxidized SO on the DE:ME ratio, N digestibility, or N retained as a percent of N digested, on the urinary ratio of lactulose to mannitol, on serum, urinary, or liver thiobarbituric acid reactive substances, on plasma protein carbonyls, or on urinary or liver 8-OH-2dG (P > 0.10). In experiment 2, pigs fed the SO thermally processed at 90 °C had the greatest isoprostane concentrations in the serum (P ≤ 0.01) and urine (P ≤ 0.05) compared to pigs fed the unprocessed SO. These results indicate that the change in fatty acid composition and/or the presence of lipid peroxidation products in peroxidized SO may reduce ADG and ADFI in nursery pigs, but appears to have no impact on GE, lipid, or N digestibility, or gut permeability. These data suggest that the presence of lipid peroxidation products may affect certain markers of oxidative stress.

Influence of feeding thermally peroxidized soybean oil on growth performance, digestibility, and gut integrity in growing pigs.

Consumption of highly peroxidized oils has been shown to affect pig performance and oxidative status through the development of compounds which differ according to how oils are thermally processed. The objective of this study was to evaluate the effect of feeding varying degrees of peroxidized soybean oil (SO) on parameters of growth performance; lipid, N, and GE digestibility, gut integrity in growing pigs, and plasma Trp. Fifty-six barrows (25.3 ± 3.3 kg initial BW) were randomly assigned to one of four diets containing either 10% fresh SO (22.5 °C) or thermally processed SO (45 °C for 288 h, 90 °C for 72 h, or 180 °C for 6 h), each with an air infusion of 15 L/min. Peroxide values for the 22.5, 45, 90, and 180 °C processed SO were 2.0, 96, 145, and 4.0 mEq/kg, respectively; 2,4-decadienal values for 22.5, 45, 90, and 180 °C processed SO were 2.11,5.05, 547.62, and 323.57 mg/kg, respectively; and 4-hydroxynonenal concentrations of 0.05, 1.05, 39.46, and 25.71 mg/kg with increasing SO processing temperature. Pigs were individually housed and fed ad libitum for a 49 d period to determine the effects of SO peroxidation status on growth performance, including a metabolism period for assessing GE and N digestibility, and N retention. In vivo urinary lactulose to mannitol ratio was also assessed to evaluate potential changes in small intestinal integrity. Although there were no differences observed in ADFI (P = 0.19), ADG was decreased in pigs fed 90 °C SO diet (P = 0.01), while G:F was increased (P = 0.02) in pigs fed 45 °C SO diet compared to the other SO diets. Pigs fed the 90 °C processed SO had the lowest (P = 0.01) DE as a percentage of GE, whereas ME as a percentage of DE was lowest (P = 0.05) in pigs fed the 180 °C SO and 90 °C SO followed by 45 °C SO and fresh SO. Ether extract (EE) digestibility was lowest (P = 0.01) in pigs fed 90 °C SO followed by pigs fed 180 °C SO, 45 °C SO, and fresh SO. The percent of N retained was greatest (P = 0.01) in pigs fed fresh SO followed by pigs fed 45 °C SO, 180 °C SO, and 90 °C, respectively. There were no differences observed among SO treatments for urinary lactulose to mannitol ratio (P = 0.60). Pigs fed SO processed at 90 °C and 180 °C had lower concentrations (P < 0.01) of serum Trp compared to pigs fed the 22.5 °C and 45 °C SO treatments. The presence of lipid peroxidation products, namely several aldehydes, contained in the 90 °C SO diet reduced ADG, GE and EE digestibility, and N balance, but had no impact on gut permeability.