Vitamin D status and the risk of neuromyelitis optica spectrum disorders: A systematic review and meta-analysis.

BACKGROUND: Previous studies have linked vitamin D deficiency with autoimmune diseases, and recent research has found low vitamin D levels in neuromyelitis optica spectrum disorder (NMOSD) patients. We aimed to determine the variances in serum 25(OH)D levels between NMOSD patients and healthy controls. METHODS: We searched English and Chinese databases (PubMed, Embase, Cochrane Library, Web of Science, CBM, CNKI, WanFang Med, VIP) for observational studies related to serum 25(OH)D levels in NMOSD patients published up to August 24, 2023. We included studies with healthy controls and compared serum 25(OH)D levels between NMOSD patients and controls. We computed the mean difference (MD) and 95% confidence interval (CI) for continuous variables to evaluate serum 25(OH)D levels and combined odds ratios (ORs) and 95% CIs for dichotomized 25(OH)D data. RESULTS: Six papers were selected for meta-analysis, including 794 participants (347 in the NMOSD group and 447 in the healthy control group). Meta-analysis showed significantly lower serum 25(OH)D levels in the NMOSD group (MD: -7.83, 95 % CI: -10.99 to -4.68). The risk of 25(OH)D deficiency was 23.36 times higher in the NMOSD group (OR: 23.36, 95 % CI: 0.85 to 640.76, p = 0.06>0.05), with a 94 % occurrence rate. There was no significant difference in the risk of having sufficient 25(OH)D between the groups (p = 0.12>0.05). CONCLUSION: NMOSD patients have lower serum 25(OH)D levels than healthy controls. However, the current research results do not provide evidence for a causal relationship between serum 25(OH)D levels and the onset of NMOSD. Routine vitamin D supplementation may be advantageous for patients with NMOSD.

Effects of Bone Grafting and Non-Bone Grafting on Implant Stability and New Bone Formation in Patients Undergoing Maxillary Sinus Floor Elevation Combined with Bicon Short Implants.

Objective: To compare the effects of bone grafting versus non-bone grafting on implant stability and new bone formation in patients undergoing maxillary sinus floor lift combined with placement of a Bicon short dental implant. Methods: We recruited 60 patients with posterior maxillary tooth loss and insufficient jaw bone mass from December 2017 to December 2019, and the patients were divided into 2 groups in accordance with the surgical method: the bone grafted group (n = 32) and the non-bone grafted group (n = 28). Both groups underwent maxillary sinus floor elevation combined with Bicon short dental implant placement. No bone-grafting materials were used in the non-bone grafted group, and autologous bone chips mixed with Bicon bone substitute were used for bone grafting in the bone grafted group. The 2 groups were compared for their peri-implant index and periodontal bleeding index immediately after the operation, as well as at 3, 6, and 12 months postoperatively. The study also compared the sub-sinus-membrane height, peri-implant bone density, implant stability quotient, and alveolar bone height in the implant area at 3, 6, and 12 months after the operation, as well as the implant survival rate and complications (infection, bleeding, mucosal perforation, sinus-floor cyst, and bone-graft displacement) 12 months after the operation. Results: The peri-implant index and periodontal bleeding index immediately after the operation in the bone grafted group were higher than those in the non-bone grafted group (all P < .05), but there were no significant differences in the 2 indices between the 2 groups at 3, 6, and 12 months after the operation (all P > .05). The sub-sinus-membrane height, peri-implant bone density, implant stability quotient, and alveolar bone height in the bone grafted area were higher in the bone grafted group than in the non-bone grafted group at 3, 6, and 12 months after the operation (all P < .05). Although the implant survival rate in the bone grafted group was slightly higher than that in the non-bone grafted group at 12 months after the operation, the difference was not statistically significant (P > .05). One case of mucosal perforation occurred in the bone grafted group, but there was no significant difference in the complication rate between the 2 groups (P > .05). Conclusion: The findings of this study support the use of autologous bone chips mixed with Bicon bone substitute in maxillary sinus floor elevation combined with Bicon short dental implant placement for improved implant stability and new bone formation. Further research is needed to evaluate long-term outcomes and potential complications associated with this technique.

Comparison of the effect on proliferation of osteoblasts MC3T3-E1 between the concentrated growth factor extract and the platelet-rich fibrin extract. / æ¯”è¾ƒæµ“ç¼©ç”Ÿé•¿å› å­æå–æ¶²å’Œå¯Œè¡€å°æ¿çº¤ç»´è›‹ç™½æå–æ¶²å¯¹æˆéª¨ç»†èƒžMC3T3-E1增殖的影响.

OBJECTIVES: To compare the effect on proliferation of osteoblasts MC3T3-E1 between the concentrated growth factor extract (CGFe) and the platelet-rich fibrin extract (PRFe). METHODS: CGFe and PRFe were prepared. MC3T3-E1 was cultured in DMEM medium containing CGFe (10%, 20%, or 30%) and PRFe (10%, 20%, or 30%). The proliferation of MC3T3-E1 was detected by MTT assay at Day 1, 3, 5, and 7. ALP activity was detected by alkaline phosphatase (ALP) staining at Day 1, 3, 5, and 7, and mRNA expressions of Runt-related transcription factor 2 (Runx2) and Osterix (Osx) were detected by quantitative RT-PCR (RT-qPCR) at Day 3 and 7. RESULTS: Compared with the control group, CGFe and PRFe promoted the proliferation of MC3T3-E1 at Day 1, 3, 5, and 7 (all P<0.05). Except for the first day, the proliferation activity in the CGFe group was higher than that in the PRFe group (all P<0.05). At Day 1, 3, 5, and 7, compared with the control group, the ALP activities in the CGFe group and the PRFe group were significantly increased (all P<0.05). Except for the first day, the ALP activity in the CGFe group was higher than that in the PRFe group (all P<0.05). At Day 3 and 7, compared with the control group, the mRNA expression levels of Osx and Runx2 in the CGFe group and the PRFe group were significantly increased (all P<0.05); compared with PRFe group, the mRNA expression level of Osx in the CGFe group was significantly higher than that in the PRFe group, and the mRNA expression level of Runx2 was significantly lower than that in the PRFe group (all P<0.05). CONCLUSIONS: CGFe could promote the proliferation of MC3T3-E1 stronger than PRFe, which might be related to the increase of ALP activity and up-regulation of Osx expression.