RESUMEN
The present work investigated the characteristics, antioxidative, anti-inflammative and renoprotective effects of mycelia selenized polysaccharides (MSPS) from Oudemansiella radicata on lipopolysaccharide (LPS)-induced kidney damaged mice. The results demonstrated that both MSPS and mycelia polysaccharides (MPS) showed potential renoprotective effects reflecting by decreasing the serum levels of CRE, BUN and UA, increasing the nephritic enzyme activities of SOD, GSH-Px, CAT and T-AOC, reducing the activities of MPO, as well as down-regulating the contents of MDA and LPO, respectively. And the MSPS showed superior effects. Furthermore, the selenium contents and physical properties of polysaccharides were investigated by FAAS, GC, HPGPC, FT-IR, NMR and AFM analysis, and the results indicated that MSPS was a homogeneous heteropolysaccharide with an average molecular weight of 3.12×104Da. These results may provide theoretical basis in applying natural and functional foods for the prevention and alleviation of kidney damages and its complications.
Asunto(s)
Agaricales/química , Antiinflamatorios/farmacología , Antioxidantes/farmacología , Riñón/patología , Micelio/química , Polisacáridos/farmacología , Selenio/química , Animales , Nitrógeno de la Urea Sanguínea , Catalasa/metabolismo , Creatinina/sangre , Glutatión Peroxidasa/metabolismo , Riñón/efectos de los fármacos , Peroxidación de Lípido/efectos de los fármacos , Lipopolisacáridos , Espectroscopía de Resonancia Magnética , Malondialdehído/metabolismo , Ratones , Microscopía de Fuerza Atómica , Peso Molecular , Monosacáridos/análisis , Peroxidasa/metabolismo , Polisacáridos/ultraestructura , Espectrofotometría Ultravioleta , Espectroscopía Infrarroja por Transformada de Fourier , Superóxido Dismutasa/metabolismo , Pruebas de Toxicidad Aguda , Ácido Úrico/sangreRESUMEN
In order to contribute to the exploitation and utilization of spent mushroom substrates (SMS) of Laetiporus sulphureus, hot-water-extractable polysaccharides (H-SMPS) and enzymatic-extractable polysaccharides (E-SMPS) were successfully isolated from SMS of L. sulphureus. Both H-SMPS and E-SMPS were found to have high reducing power and potential scavenging activities against hydroxyl, DPPH, and superoxide anion radicals. In vivo assays showed that the administration of H-SMPS and E-SMPS has potential hepatoprotective effects against alcohol-induced alcoholic liver disease (ALD), possibly brought about by improving liver function, increasing antioxidant status, and reducing lipid peroxidation. Furthermore, monosaccharide composition analysis showed that fucose may play a vital role in guaranteeing stronger hepatoprotection. These results may provide references for the exploitation of the SMS of L. sulphureus as a source of H-SMPS and E-SMPS, which in turn can be used as functional foods or natural drugs for the prevention of ALD and other liver diseases.