Structural Determination of a New Cycloartane Glycoside from Asplenium ruprechtii.

We characterized a new cycloartane glycoside, herein known as aspleniumside F (1), along with five known compounds as kaempferol-3-O-[(6-O-(E)-feruloyl)-ß-D-glucopyranosyl]-(1→2)-ß-D-galacopyranoside (2), quercetin-3-O-[(6-O-(E)-feruloyl)-ß-D-glucopyranosyl]-(1→2)-ß-D-glucopyranoside (3), kaempferol-3-O-[(6-O-(E)-caffeoyl)-ß-D-glucopyranosyl]-(1→2)-ß-D-glucopyranoside (4), kaempferol-3-O-[(6-O-(E)-caffeoyl)-ß-D-glucopyranosyl]-(1→2)-ß-D-glucopyranosyl-7-O-ß-D-glucopyranoside (5), and kaempferol-3-O-[(6-O-p-coumaroyl)-ß-D-glucopyranosyl]-(1→2)-ß-D-glucopyranosyl-7-O-ß-D-glucopyranoside (6), from Asplenium ruprechtii Sa. Kurata, a folk medicine widely used to treat Thromboangiitis obliterans in China, Japan, and Korea. Based on spectroscopic, mainly 1D-, 2D-NMR and (+)-HR-ESI-MS, analyses as well as through comparisons with previous reports, its chemical structure was determined as 3ß,24,30-tri-ß-D-glucopyranosyl-23,25-dihydroxycycloartane (= (23R,24R)-3ß,24-bis-(ß-D-glucopyranosyloxy)-23,25-dihydroxy-9ß-9,19-cyclolanostan-29-yl ß-D-glucopyranoside). According to the 1 H coupling constant of anomeric protons and co-TLC of the acid hydrolysate with D-glucose, all three glycoside groups in 1 were revealed as ß-D-glucopyranosyl. Furthermore, SOD-like antioxidant activity evaluation via IC50 of 12.43, 6.78, 9.12, 6.94 and 4.85 µM revealed that compounds 2-6 had bioactivity.

Intervention effect of traditional Chinese medicine Yi Tang Kang on metabolic syndrome of spleen deficiency.

OBJECTIVE: To investigate effects of herbal compound Yi Tang Kang on the spleen deficiency metabolic syndrome. METHODS: Forty male Wistar rats were randomly divided into two groups: the normal control group and the MS spleen deficiency syndrome group. The control group rats were fed with standard diet and water, while MS spleen deficiency syndrome group with high fat diet and low dose intraperitoneal injection of streptozocin, which swam to the endurance limit. After 12 weeks, the MS spleen deficiency syndrome group was randomly divided into two groups, with 13 rats in each group. Rats in model group were fed with high fat diet and continuouly administered with daily saline, and rats in intervention group with high fat diet were trated with traditional Chinese medicines Yi Tang Kang by gavage, 2 mL/200 g at the same time every day. 10 weeks later, the expression of serum proteomics was investigated through abdominal aortic puncture and separation of serum, using isotope labeling technique, high performance liquid chromatography and four bar-Orbitrap mass spectrometer. RESULTS: After treatment with traditional Chinese medicine yitangkang, in the model group, important carboxylesterase and retinal guanylate cyclase 2 precursor were upregulated. As for intervention group, these indesxes were raised, but immunoglobulin IgG, carnitine acetyltransferase, tubulin beta -5, and Gan Lu sugar binding protein C were down-regulated. At the same time, some new biological active substances, such as protein tyrosine kinase, beta glucosidase were also found. CONCLUSIONS: Traditional Chinese medicines Yi Tang Kang could regulate glucose and lipid metabolism in rats with spleen deficiency syndrome.